presented by Dr Hadeel F Gad Leishmania and Mycobacterium TB share many similarities in their pathogenesis and both pathogens are macrophage parasites Furthermore coinfection by the two pathogens is not uncommon in clinical practice in East Africa and in other parts of the wo ID: 482861
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Slide1
In-vitro Analysis of cytokines responses of visceral leishmaniasis and pulmonary tuberculosis patients to homologous and heterologous antigen stimulation
presented by
Dr
Hadeel
F. GadSlide2
Leishmania
and Mycobacterium TB share many similarities in their pathogenesis and both pathogens are macrophage parasites.
Furthermore,
co-infection by the two pathogens is not uncommon in clinical practice in East Africa and in other parts of the world (Sati 1942; el-Safi et al. 1995; Khalil et al. 1998,
Bryceson
et al.
1985Slide3
Little is known about the immunological responses of Visceral Leishmaniasis and pulmonary Tb patients to homologous and heterologous antigen stimulation. Slide4
Visceral
Leishmaniasis
, also noun as
kala-azarm
is usually an
inidious
chromic disease among the inhabitants of endemic areas.
Tuberculosis is known as a major cause of morbidity and mortality worldwide.Slide5
The
present study was carried out to analyze in-vitro
Th-1
and Th-2 cytokines responses of visceral leishmaniasis (VL) and pulmonary tuberculosis (TB) patients to homologous and heterologous antigens stimulates
.Slide6
The cytokine profiles of confirmed VL patients, pulmonary TB patients and healthy individuals were compared after stimulation with live
Leishmania
promastigotes and BCG.
Th-1
(IFN-γ and TNF-α), Th-2 (IL-10) and inflammatory cytokine IL-15 were measured in the supernatants of stimulated whole blood samples
by ELISA
. Slide7
The concentration of Th-1 cytokines (IFN-γ and TNF-α) were significantly higher in the supernatants of stimulated whole blood of VL patients compared with TB patients mainly when stimulated by homologous
L.donovani
antigen. Slide8
Cytokines profiles of visceral
leishmaniasis
, pulmonary tuberculosis and healthy controls whole blood samples stimulated with live
L.donovani
promastigotes
. Slide9
Stimulation of VL, TB and healthy controls samples with BCG resulted in significant production of IFN-γ and TNF-α by VL samples while induced a significant IL-10 production by TB patients’ samples. No significant IL-15 was produced by either VL, TB or healthy controls samples.Slide10
Th-2 cytokine IL-10 was significantly produced by whole blood of TB patients following BCG stimulation. Both VL and TB patients produced significant concentration of IL-15 when stimulated with homologous and heterologous antigens.Slide11
VL
and pulmonary TB patients responded effecticiently to homologous antigen stimulation. While VL patients mounted protective immune response (TH1), TB patients mounted TH2 response.