Lemke RAS et al A bacterial biosynthetic pathway for methylated furan fatty acids Journal of Biological Chemistry 295 97869801 2020 DOI 101074jbcRA120013697 ID: 919050
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Bacterial production of furan fatty acids
Lemke
, R.A.S., et al. “A bacterial biosynthetic pathway for methylated furan fatty acids.” Journal of Biological Chemistry 295, 9786-9801 (2020). [DOI: 10.1074/jbc.RA120.013697]
Objective Decipher the bacterial furan fatty acid biosynthetic pathway by identifying the genes necessary and sufficient to produce specific furan fatty acid products and intermediates.
Results/ImpactsGLBRC researchers have described a previously unknown bacterial biosynthetic pathway for furan fatty acid biosynthesis, including identification of enzymes, intermediates, and cofactors. These studies provide important new insights into the biosynthesis of furan rings, information to predict the presence of similar biosynthetic pathways, and identify genes that can be used to engineer increased production of furan fatty acids for use as fuel additives, lubricants, and other industrial products.
BRC Science Highlight
GLBRC July 2020
ApproachUse gas chromatography–mass spectrometry (GCMS) to determine the phospholipid composition of a series of mutants to identify the genes and cofactors needed for individual steps in production of furan fatty acids. Identify the chemical structures of intermediates in the bacterial furan fatty acid biosynthetic pathway. Conduct isotopic studies to determine the source of the oxygen in the furan ring of these acids.
GLBRC scientists identified important steps in the production of furan fatty acids in the cellular membrane of the bacterium
Rhodobacter
spheroides.