Principle there is interaction between the light and sample particle spectrophotometer is employed to measure the amount of light that a sample absorbs spectrophotometer consists of two ID: 560854
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Slide1
SPECTROPHOTOMETRYSlide2
Principle
:
there is interaction between the light and sample particle ,
spectrophotometer
is employed to measure
the amount of light that a sample absorbs
. Slide3
spectrophotometer consists of two
instruments:
1-
spectrometer
for producing light of any selected color (
wavelength
2-
photometer
for
measuring the intensity of light.
The
instruments are arranged so that liquid in a
cuvette
can be placed between the spectrometer beam and the photometerSlide4
The most common spectrophotometers are used in the
UV
and
visible
regions of the spectrum
.Slide5
Light source
:
Two kinds of lamps
1-
Deuterium lamp
in UV- spectrophotometer
spectrophotometer
-
2-
tungsten lamp
in visibleSlide6
Sample cell
:
A container that contains a sample is usually called "cell“
two types are available:
1-
glass
used in
v
isible spectrophotometer
2-
quartz
used in UV spectrophotometer
.Slide7
Application :
1- qualitative :
The wavelength of maximum conc. give information about structure in
λ
max
2- quantitative :
Determination of conc. By beer’s law
A =
abc
Slide8
Sample and standard solution
:
To measure the concentration of a substance, a sample of (unknown concentration) is compared with known concentration (standard solution) to determine the concentration .
The blank contain all the substance
except
the
analyte