Casein Enzymic Hydrolysate510LAsparagine510Potato Flour 3640Skim Milk 10000Malachite Green 120Final pH at 25C 70 02Precautions and DisclaimerFor laboratory use only Not ID: 961782
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PETRAGNANI MEDIUM BASEProduct Number P8851Product DescriptionPetragnani Medium Base with supplements is used forthe isolation and cultivation of Mycobacteria other thanMycobacterium leprae. Casein enzymic hydrolysate,potato flour and skim milk supply the amino acids,proteins and carbohydrates for the growth ofMycobacteria. L-asparagine initiates growth andincreases the growth rate, while glycerol providesenergy to the Mycobacteria. Egg yolk emulsionprovides lipids required for metabolism. Malachitegreen inhibits most of the bacteria exceptMycobacteria.Item g/L Casein Enzymic Hydrolysate5.10L-Asparagine5.10Potato Flour 36.40Skim Milk 100.00Malachite Green 1.20Final pH (at 25C) 7.0 0.2Precautions and DisclaimerFor laboratory use only. Not for drug, household orother uses.Preparation InstructionsSuspend 147.8 grams of Petragnani Medium Base in1000 mls of distilled water containing 60 mls of(G6279) Glycerol. Sterilize by autoclaving at 15 lbspressure (121C) for 15 minutes. Aseptically prepare1.277 liters of an emulsion of whole egg. Mix the wholeegg emulsion with 121 mls of (E7899) Egg YolkEmulsion and aseptically add to the 1000 mls ofsterilized and cooled Petragnani Medium Base. Mixwell and dispense into tubes. Inspissate at 85C for 45minutes.StorageStore the dehydrated medium at 24prepared medium at 2-8Product ProfileAppearance Bluish green colored,homogeneous, freeflowing powder.Color and ClarityThe mixture of sterilebasal medium and wholeegg emulsion, wheninspissated, coagulates toyield pale bluish greencolored opaque smoothslants.Cultural ResponseCultural characteristicsare observed after 1-8weeks at 35C in a 5%-environment.Organisms GrowthMycobacterium tuberculosis good-luxuriantMycobacterium kansassi good-luxuriantMycobacterium smegmatis good-luxuriantMycobacterium fortuitum good-luxuriantReferences1. Norton, J.F. et al., (1932). Am. Rev. Tuberc. 25,MacFaddin, J.F. (1985). Media for Isolation-Cultivation-Identification-Maintenance of MedicalBacteria. Vol. 1. Williams and Wilkins. Baltimore,Maryland. Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchasermust determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.