Cytokines are rapidly secreted upon expression which results in low not detectable intracellular concentrations The use of secretion inhibitors allows for intracellular accumulation of cytokines ID: 999281
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1. Trapping Intracellular Cytokines®Cytokines are rapidly secreted upon expression which results in low / not detectable intracellular concentrations.The use of secretion inhibitors allows for intracellular accumulation of cytokines:Brefeldin A (#420601) - inhibits transport of proteins from ER to Golgi.Monensin (#420701) - is polyether antibiotic that blocks intracellular protein transport.Secretion inhibitors are toxic. Exposure of cells should be as short as possible! (2-24h)