PDF-Enzyme Technical Association

Reprints permitted INTRODUCTION Enzymes have played an important role ine since the dawn of of life itself Civilizations have used enzymes for thunderstanding what they several generations science. C483 Spring 2013. Questions. 1. Which . statement is false about allosteric regulation? . A) It is usually the mode of regulation for the last step in reaction pathways since this step produces the final product. . Lecture 3. Objective. To understand . Specificity of enzymes. Specificity means:. Ability of an enzyme to . catalyse. a specific reaction and NO others. The active site. The . active site, . is. . Chapter 2: Section 2.5. 1. Objectives. SWBAT explain the effect of a catalyst on activation energy. . SWBAT describe how enzymes regulate chemical reactions. . and maintain homeostasis.. 2. Starter: How can this be possible. immobilised enzyme enzyme 21. An enzyme specifically through a this respect, lipoamide dehydrogenase particularly useful enzyme comprises two polypeptide chains each binding binding and containing 10 Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER 3. Immobilized . enzymes and their . uses. 2. Enzyme reactors. An enzyme reactor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymatic means (Figure 5.1). . Competitive & Non-Competitive. Learning Objectives. To learn about what enzyme inhibition is.. To learn how competitive and non-competitive inhibitors affect the active site.. To understand the implications of inhibition of rates of reaction.. Inhibition is a term used to describe the inability of a product being formed due to the presence of another substance (the inhibitor). Enzyme inhibition . can be competitive or noncompetitive. Competitive inhibition is caused when an inhibitor “competes” with the substrate in binding with the enzyme. Pratt & . Cornely. . Ch. 7. Other Factors. Other factors affect enzyme activity. Temperature. pH . pH Optimum. Determined by structural stability. Compartmentalization. Determined by active site residues. Competitive & Non-Competitive. Learning Objectives. To learn about what enzyme inhibition is.. To learn how competitive and non-competitive inhibitors affect the active site.. To understand the implications of inhibition of rates of reaction.. Learning Objectives. Learn how the rate of an enzyme controlled reaction is measured.. Learn how temperature affects the rate of an enzyme controlled reaction.. Learn how pH affects the rate of an enzyme controlled reaction.. Biology. Do not appreciate the awesomeness of enzymes!!!!. How do enzymes work?. Lock and key theory. Enzymes have a specific 3D structure. They exactly match the shape of a molecule of substrate. How do enzymes work?. Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER 1. Fundamentals of Enzymes. 2. Fundamentals of enzymes. Why enzymes?. Enzyme nomenclature. Enzyme . units. Sources of . Objectives. To monitor the progress of an enzyme catalyzed reaction (Acid phosphatase).. To determine the initial rate of the reaction (. V. i. ).. Important terms and points:. Enzyme kinetics. The central approach for studying the mechanism of an enzyme-catalyzed reaction is to study enzyme kinetics. . In this experiment, we will continue to study acid phosphatase kinetics.. Objective:. To establish the relationship between enzyme concentration and the rate of an enzyme catalyzed reaction.. The reaction rate will increase as the concentration of enzymes is increased but there must be a .