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The Accellix Platform automates your entire GMP cell


phenotyping process from sample preparation to data acquisition and analysis to generate rapid results directly in the manufacturing suite Central to our enabling technology are standard and custom as

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Document on Subject : "The Accellix Platform automates your entire GMP cell"— Transcript:

1 The Accellix Platform automates your ent
The Accellix Platform automates your entire GMP cell phenotyping process from sample preparation to data acquisition and analysis, to generate rapid results directly in the manufacturing suite. Central to our enabling technology are standard and custom assays. These assays enable sample preparation in a stable, single-use microuidic cartridge using unitized and dried reagents that are stable at ambient temperatures. The dried reagent also has control beads, enabling cell counting and in-run QC for every assay. Each assay incorporates an Accellix cartridge with a unique QR code, simplifying the INTENDED USE The T Cell Subset assays are used for the enumeration (frequencies and counts) of various T cell subsets including, viable CD45+ leukocytes, CD3+ T cells, CD4+ T helper cells, and CD8+ cytotoxic T cells. The lyse (L) assays may be used for staining apheresis, whole blood, and other samples that require differential RBC (red blood cell) lysis, while the non-lyse version may be used for staining puried white blood cells. N- 1 and N-2 test kits contain only CD45 and CD3 antibodies, enabling customers to Platform. The assays have been formulated to process samples with cell concentrations ranging between 1 -50 million cells/mL. A detailed protocol on how to run the Accellix assays can be found in the Accellix Assay Kit Instructions for Use. KEY BENEFITS + Each assay incorporates an Accellix cartridge with a unique QR code, simplifying the workow and reducing the chances for + Unitized, dried-down reagents are stable at ambient temperature, and increase repeatability and accuracy. + Each assay contains a viability dye and count beads, obviating the need for separate viable cell counting and providing in-run QC. T CELL SUBSET ASSAYS CONTENTS lyse or non-lyse buffer Dried reagents in tube STORAGE Dry cool place protected from light DESCRIPTION MARKER FLUOROPHORE CLONE CD45 PE/Cyanine7 HI30 CD3 PE/Cyanine5 UCHT- 1 CD4 PE RPA-T4 CD8 PE/Dazzle TM 594 RPA-T8 Viability dye N/A Count beads N/A N/A 1 . 2 . DATA SHEET GET IN TOUCH AT INFO@ACCELLIX.COM OR LEARN MORE AT ACCELLIX.COM GET I

2 N TOUCH AT INFO@ACCELLIX.COM OR LEARN
N TOUCH AT INFO@ACCELLIX.COM OR LEARN MORE AT ACCELLIX.COM DATA SHEET IMMUNO-TROL control blood was stained with T Cell RTF Assay (L) CATALOG NUMBER ASSAY NAME DESCRIPTION CONTENTS A 1 000- 1 L T Cell RTF Assay (L) Full T Cell panel with lysis buffer for apheresis or samples requiring RBC lysis CD45-AF488, CD3-PECy5, CD4-PE, CD8-PED594, DiYO-3 Viability, Control Beads A 1 00 1 - 1 NL T Cell RTF Assay (NL) Full T Cell panel with non-lyse buffer for puried cells or drug product CD45-AF488, CD3-PECy5, CD4-PE, CD8-PED594, DiYO-3 Viability, Control Beads A 1 00 1 - 1 L T Cell N-1 AF488 RTF Test Kit (L) Basic T cell panel with lysis buffer for apheresis or samples requiring RBC lysis; user adds liquid AF488 conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid AF488 conjugate added by user) A 1 00 1 - 1 NL T Cell N-1 AF488 RTF Test Kit (NL) Basic T cell panel with non-lyse buffer for puried cells or drug product; user adds liquid AF488 conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid AF488 conjugate added by user) A 1 00 1 - 2 L T Cell N-1 PE RTF Test Kit (L) Basic T cell panel with lysis buffer for apheresis or samples requiring RBC lysis; user adds liquid PE conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid PE conjugate added by user) A 1 00 1 - 2 NL T Cell N-1 PE RTF Test Kit (NL) Basic T cell panel with non-lyse buffer for puried cells or drug product; user adds liquid PE conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid PE conjugate added by user) A 1 00 1 -3L T Cell N-2 AF488 PE RTF Test Kit (L) Basic T cell panel with lysis buffer for apheresis or samples requiring RBC lysis; user adds liquid AF488 and PE conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid AF488 and PE conjugates added by user) A 1 00 1 -3NL T Cell N-2 AF488 PE RTF Test Kit (NL) Basic T cell panel with non-lyse buffer for puried cells or drug product; user adds liquid AF488 and PE conjugate antibody CD45-PECy7, CD3-PECy5, DiYO-3 Viability, Control Beads (liquid AF488 and PE conjugates added by user)