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��page of 3Reviewed 201Environmental Health and Safety2408 WandaDaley DriveAmes, Iowa 50011Phone: 515 294www.ehs.iastate.eduSharps and Biohazardous Waste ProcedureAll generators of biohazardous waste must strictly adhere to the following ISU waste disposal guidelines.Biohazardous waste is defined as:All biologically contaminated waste that could potentially cause harm to humans, domestic or wild animals or plants. Examples include humanand animalblood, tissues,and certain body fluids, recombinant DNA, and human, animal or plant pathogens. How to handle biohazardous waste:All biohazardous waste must be decontaminated before disposal. Common decontamination methods include heat sterilization (e.g., autoclaving), chemical disinfection and incineration.Animal carcassesincludingsheep and goatsand any other animals infected with humanpathogens)tissues, bedding Collect in leakproofcontainerslined with a thick trash bag, label with a “Waste for Incineration” tag, and take to the incinerator at 1676 Veterinary Medicine for incineration.Collect all transgenic animal carcasses or animal carcasses, tissuesand bedding, infected withhumanpathogensleakproofbiohazard bags or containerslined with a thick trash bagLabel with a “Waste for Incineration” tag, and take to the incinerator at 1676 Veterinary Medicine for incineration.B.LiquidsDecontaminate all liquid biohazardous materials (such as human blood, bacterial cultures in liquid media, body fluids of animals experimentally Example of “Waste for Incineration” tag �� &#x/Att;¬he; [/;ott;&#xom ];&#x/BBo;&#xx [4;.9;' ;A.5;ࠣ ;ց.;鞁&#x 66.;馄&#x ]/S;&#xubty;&#xpe /;oot;r /;&#xType;&#x /Pa;&#xgina;&#xtion;&#x 000;&#x/Att;¬he; [/;ott;&#xom ];&#x/BBo;&#xx [4;.9;' ;A.5;ࠣ ;ց.;鞁&#x 66.;馄&#x ]/S;&#xubty;&#xpe /;oot;r /;&#xType;&#x /Pa;&#xgina;&#xtion;&#x 000;page of 3Reviewed 201Examples Biohazard Biohazard symbol with “X”Afterautoclaving, place the now decontaminated biohazard bagintoa dark garbage bag, seal, and place in regular trash.Nondisposable or reusable itemsDecontaminate nondisposable or reusable items (such as equipment, glassware, bench topsetc.) contaminated with biohazardous materials by using a chemical disinfectant (such as 10% bleach, a quaternary ammonium compound, an alcohol, etc.). Choose a chemical disinfectant appropriate for the specific biohazardous material being usedand allow for sufficient contact timeMetal SharpsUse separate containers for metal, glassplasticsharps.Collect all metal sharps (such as hypodermic needles, surgicalneedles, scalpel blades, razor blades, etc.) contaminated with biohazardous materials in leakproof, puncture resistant containers which have been labeled with the universal biohazard symbol.Decontaminate the containers by autoclaving. NOTE: To prevent needle sticks, do NOTcap the needles or remove from syringes, insteaddiscard the entire unit into the sharps waste container designated for biohazardous sharpsAfter autoclaving, label the now decontaminated sharps waste containers with a “NoInfectious Syringes and Metal Sharps Only”labelCollect metal sharps that have never been contaminated with biohazardous materials (e.g., used only with chemicals) in leakproof, puncture resistant white plastic containers labeledwith a“NonInfectious Syringes and Metal Sharps Only”labelDo not autoclave these containers, because they will meltTo dispose of metal sharps, submit Waste Removal requestall Ames Laboratory ESH at (515) for AmesLaboratory pick up. EH&S will only pick up metal sharps waste that has been decontaminated. ��page of 3Reviewed 201DO NOT REUSE SHARPSWASTE CONTAINERS. DO NOT PUT ANY METALSHARPS WASTE IN REGULAR TRASH OR DUMPSTERS. NOTESharps containers and labels are available at Central Stores.Label examplesGlass SharpsUse separate containers for metal, glassplasticsharps. Collect all glass sharps (such as Pasteur pipets, broken glass, etc) contaminated with biohazardous materials in leakproofpuncture resistant containers which have been labeled with the universal biohazard symbol (see example above).Decontaminate the containers by autoclaving.After autoclaving, empty the now decontaminated glass sharps container into a yellow tidy cat container in your laboratoryfor storageor into the yellow glass disposal bin on your building’s loading dockfor disposalCallFP&M at(515) 295100 for removalwhen the bin is fullCollect glasssharps that have never been contaminated with biohazardous materials used only with chemicals) in a yellow tidy cat container in your laboratory for storage or into the yellow glass disposal bin on your building’s loading dock for disposalAutoclaving of these containers is not necessary.Call FP&M at (515) 2945100 for removalwhen the bin is fullPlastic SharpsUse separate containers for metal, glassand plastic sharps. Collect plastic materials (pipette tips, plastic pipettes) that can poke out of bags and contaminated with biohazardous materials in leakproof, puncture resistant containers which have been labeled with the universal biohazard symbol (see example above). Decontaminate the containers by autoclaving.After autoclaving, place the now decontaminated plastic sharps inside garbage bag linedcardboard box, seal, label “Plastic Sharps”and throw into the regular trash dumpster.DO NOT REUSE SHARPSWASTE CONTAINERSThis procedureapplies only to potentially biohazardous waste streams. Radioactive wastes and EPA regulated chemical wastes should continue to be handled as specified in the Iowa State UniversityRadiation Safety Manualand the Iowa State University Waste and Recycling Guidelines , respectively. Contact EH&S at (515) or Ames Laboratory ESHat (515) 294with any questions or concerns regarding waste disposal.