PPT-NS5A and polymerase inhibitors

Mark Sulkowski MD Professor of Medicine Johns Hopkins University Baltimore Maryland 21212 Case 57 yo woman with genotype 1a and bridging fibrosis PROVE1 study treated with TVR x 12 . nudlernyumcorg DOI 101016jcell201206003 RNA polymerase is a ratchet machine that oscillates between productive and backtracked states at numerous DNA positions Since its 64257rst description 15 years ago backtrackingthe reversible sliding of RNA poly (PCR). PCR. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone). Allows scientists to isolate pure quantities of specific DNA sequences. Mark Sulkowski, MD. Associate Professor of Medicine . Johns Hopkins University School of Medicine . Limitations of PegIFN + Ribavirin . (with or without protease inhibitors). Antiviral activity is host + virus genotype dependent . Kit P6 v2. January 15, 2015. DNA/Polymerase Binding Kit . P6 v2. Observed lower productive fraction with P6 compared to P5, requiring higher concentration to achieve similar productivity as P5. Goal: . 322 BCH. Exp. (8). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To . study the effect of inhibitors on the rate of an enzymatic reaction.. To . determine the type of inhibition of acid phosphatase by inorganic phosphate and sodium fluoride. . Matthew Luu, Yuan Fang (Pearl) Cai, Shiming Sun, . Ziyi Cheng. PHM142 Fall . 2016. Coordinator. : Dr. Jeffrey Henderson. Instructor: Dr. David Hampson. What is Hepatitis C? . Flaviviridae. virus family. Nahla . Bakhamis. Multiple copies of specific DNA . sequences;. . ‘Molecular Photocopying’ . Polymerase Chain Reaction. 1983;. In . vitro. enzymatic amplification of specific DNA sequences from . . . University of Witwatersrand. Dr Nathan October. One sneeze can generate an aerosol of enough cold viruses to infect thousands of people!. Treatment of . Herpesviruses. . Varicella-zoster,. Cytomegalavirus,. Herpes simplex. Anti-metabolites . . “False” DNA building blocks . (MK-1439; DOR). . NNRTI. Merck. Filed with USFDA. Cabotegravir-LA. (GSK-744; CAB). For . PrEP. INI. ViiV. Topical microbicide. Oral. Long-acting (LA) parenteral. 1. Fostemsavir. (BMS-663068). Prodrug of . (GSK-744; CAB). For . PrEP. INI. ViiV. Topical microbicide. Oral. Long-acting (LA) parenteral. 1. Fostemsavir. (BMS-663068). Prodrug of . BMS-626529. Attachment inhibitor. BMS .  ViiV. MK-8591 (. EFdA. 322 BCH. Exp. (8). In this experiment, we will continue to study . acid phosphatase . kinetics.. Objectives. To . study the effect of inhibitors on the rate of an enzymatic reaction.. To . determine the type of inhibition of acid phosphatase by inorganic phosphate and sodium fluoride. . PYF12 3/21/05 8:04 PM Page 191 repressor, activation of the operon by cAMP-CAP, and control of the operon bypost-transcriptional, and post-translational regulatory mechanisms and will be usedto dem A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional .