chemoselectivity at interfaces Dmitry Eremin Fundamentals of protein ionization Used DMSO solution to assist protein ionization and improve signaltonoise ratio Eremin DB and Fokin ID: 930689
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Slide1
Eremin’s
research focuses on cutting-edge techniques to understand the origin of
chemoselectivity at interfaces.
Dmitry Eremin
Fundamentals of protein ionizationUsed DMSO solution to assist proteinionization and improve signal-to-noise ratio
Eremin, D.B. and
Fokin, V.V. J. Am. Soc. Mass Spectrom. 2021
Eremin, D.B. and Fokin, V.V. J. Am. Chem. Soc. 2021
Chemical reactions at the interfaces
Developed
the first on-water
chemoselectivity
switch in microdropl
ets
Covalent protein-ligand complexesApplies a combination of proteomics tools from peptide mapping to native MS to discover new covalent protein-ligand complexes for GPCR and thioredoxin proteins
Slide2Eremina’s research focuses on:
Development of a new multimodal imaging strategy for rapid molecular mapping of histology samples
Olga Eremina
Development of novel molecular imaging probeswith multimodal capabilities (Raman Nanoprobes)Eremina et al., Biomater. Sci.,
2021Eremina et al., J. Phys. Chem. Lett., 2021
Multiplexed imaging of biological samples
Investigation of tissues and tumors at subcellular resolution to
:uncover pathology insights
formulate the right therapeutic strategies for cancer treatmentreveal new prognostic and predictive biomarker correlations and cell interactions for early cancer diagnosis
Slide3Focus of his research:Investigation of the molecular effects of the posttranslational O-GlcNAc
(N-acetylglucosamine) modification involving the preparation of O-GlcNAc modified α-synuclein protein, which has
been implicated within Parkinson’s Disease.
Matthew Sarnowski
α-Synuclein forms amyloid associated with neurodegeneration in Parkinson’s disease
Goals:
Determining the structure of O-
GlcNAc-modified α-synuclein proteinsInvestigating the toxicity and pathogenicity of O-
GlcNAc modified fibersIdentifying O-GlcNAc modification that can be used for the treatment of
Parkinson’s disease through protein or peptide-based therapeutics or by increasing the levels of O-GlcNAc with inhibitors.
O-
GlcNAc Affects the Kinetics of α-Synuclein Aggregation
Lewis , Pratt et al., ACS
Chem Biol, 2017Levine, Pratt et al., Proc Natl Acad Sci USA, 2019
Slide4Alessandro
Coradini
Coradini’s research focuses on design and synthesis of minimal yeast chromosome. Importance: synthesis of minimal organisms advances understanding of the fundamental mechanisms that give rise to cellular life and
generate highly predictable cellular environments for bioengineering.
Developed: a pioneering experimental approach for capturing segments of natural chromosomes, mutagenizing them ex vivo, and reassembling them into heavily mutagenized synthetic chromosomes that replace the natural chromosomes in yeast.
Goals:Developing a foundational approach for eukaryotic chromosome minimization, focusing on Saccharomyces cerevisiae chromosome I.
map in unprecedented detail all essential genetic elements on a single eukaryotic chromosome and generate data for design and synthesis of the first minimal eukaryotic chromosome.Establishing a workflow for an entire eukaryotic genome minimization.
Clone-and-resemble approach for building synthetic chromosomes
In vivo cloning strategy
using pASC1 and CRISPR/Cas9
Reassembled cloned yeast chromosomal segments into new chromosomes through homologous recombination in transformed
cells
Fragments were mutagenized ex vivo and reassembled to generate a library of mutagenized chromosome I.