Course name ZOOBiochemistry Rohit Introduction In vertebrates glycogen is found primarily in liver and skeletal muscles Liver glycogen serves as a reservoir of glucose for other tissues when dietary glucose ID: 935754
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Slide1
Glycogenolysis
Class name – IVH
Course name - ZOO-Biochemistry
(Rohit)
Slide2Introduction
In vertebrates, glycogen is found primarily in liver and skeletal muscles.
Liver glycogen serves as
a reservoir of glucose for other tissues when dietary glucose
is not available.
This is important for neurons of brain because these cells can not use fatty acid as source of fuel.
Glycogenolysis is the process of breakdown of glycogen into glucose-6-phosphate.
Slide3Glycogen breakdown requires following enzymes
1.
Glycogen phosphorylase,
2. Glycogen debranching enzyme,
3. Phosphoglucomutase.
Slide4Glycogen Breakdown Is
Catalyzed
by Glycogen Phosphorylase
Glycogen phosphorylase
catalyzes
the
reaction in which an (1-4) glycosidic linkage between two glucose residues at a nonreducing end of glycogen undergoes attack by inorganic phosphate (Pi), removing
the terminal glucose residue as D-glucose 1-phosphate.
This is known as Phosphorolysis reaction (Because Inorganic phosphate (Pi) is used).
This is different from Hydrolysis of glycosidic bonds by amylase as water molecules is involved in it instead of Pi.
Pyridoxal phosphate is required as a cofactor in the glycogen phosphorylase reaction.
Phosphate group of pyridoxal phosphate molecule promotes attack by Pi on the glycosidic bond.
Glycogen phosphorylase acts repetitively on the nonreducing ends of glycogen branches until it reaches a point four glucose residues away from an (1-6) branch point, where its action stops.
Glycogen phosphorylase removes the terminal glucose residues from the non-reducing end of a glycogen chain.
Slide6Debranching enzyme,
formally
known as
oligo (1-6) to (1-4) glucantransferase,
catalyzes two successive reactions that transfer branches.
Once these branches are transferred and the glucosyl residue at C-6 is hydrolyzed, glycogen phosphorylase activity can continue.
First is the transferase activity, in which the enzyme removes terminal 3 of remaining glucose residues (Yellow) and transfer this to non-reducing end of another branch.
Second, the remaining glucose residue (Red) is removed by alpha(1-6) glucosidase activity of debranching enzyme.
Slide7Glucose 1-phosphate (end product of the glycogen phosphorylase reaction) is converted to glucose 6-phosphate by
phosphoglucomutase,
which catalyzes the
reversible reaction.
Slide8Regulation of glycogenolysis
Two hormones are responsible for stimulating the breakdown of glycogen in muscle and liver.
1. Glucagon (secreted by pancreas)
2. Epinephrine (secreted by adrenal medulla of kidney)
Liver is more responsive to glucagon whereas muscle is more to epinephrine.
Glycogen phosphorylase is regulated Hormonally.
glycogen phosphorylase of skeletal
muscle exists in two interconvertible forms:
1. Glycogen phosphorylase
a,
which is catalytically active, and
2.
Glycogen phosphorylase
b,
which is less active.
Slide9Regulation of Glycogen phosphorylase (Glycogenolysis) by Covalent modification (reversible phosphorylation which is responsive to hormones)
During vigorous muscular activity the hormone epinephrine triggers phosphorylation of a specific Ser
residue in phosphorylase
b,
converting it to its more active
form, phosphorylase
a.
When the muscle returns to rest, a second enzyme,
phosphorylase
a
phosphatase,
also called
phosphoprotein phosphatase 1 (PP1),
removes the phosphoryl groups from phosphorylase
a,
converting it to
the less active form, phosphorylase
b.
Slide10How hormones works in regulation of Glycogen breakdown
By binding to specific surface receptors, either epinephrine acting on a myocyte (left) or glucagon acting on a hepatocyte (right) activates
a GTP-binding protein Gs
It triggers cAMP
In response to cAMP, protein kinase A get activate
Finally phosphorylase b kinase becomes active and in turn activate Glycogen phosphorylate a (Active form)
Glycogen breakdown initiated
Slide11Glucogen
synthase (Glycogenesis) regulation by phosphorylation/dephosphorylation
Like glycogen phosphorylase, glycogen synthase can exist
in phosphorylated and dephosphorylated forms
Its active form,
glycogen synthase
a,
is
unphosphorylated
.
Phosphorylation of the hydroxyl side chains of several Ser residues of both subunits converts glycogen synthase
a
to
glycogen synthase b, which is inactive.Glycogen synthase kinase 3 (GSK3) is responsible for phosphorylation of serine residue of Glycogen synthase and making it inactive.
Slide12Glycogen synthase kinase 3 works only when another kinase called casein kinase II (CKII) phosphorylate Glycogen synthase on nearby residues and this process is known as priming.
Phosphoprotein phosphatase 1 (PP1), converts
glycogen synthase
b
to the
active form.
Glucose 6-phosphate binds to an allosteric site on glycogen synthase
b,
making the enzyme a better substrate for dephosphorylation by PP1 and causing its activation.
Slide13Phosphoprotein Phosphatase 1 Is Central to Glycogen Metabolism
A single enzyme, PP1, can remove phosphoryl groups from all three of the enzymes phosphorylated in response to glucagon (liver) and epinephrine (liver and
muscle): phosphorylase kinase, glycogen phosphorylase, and glycogen synthase.
PP1 is itself subject to covalent and allosteric regulation.
It is inactivated when phosphorylated by PKA and is allosterically activated by glucose 6-phosphate