CHAPTER 36 TRANSPORT IN PLANTS - PowerPoint Presentation

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CHAPTER 36

TRANSPORT IN PLANTS

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Overview of Transport in a Vascular Plant

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TERMS TO BE REVIEWED

1. passive transport 10. chemiosmosis

2. transport proteins 11. proton pump

3. active transport 12. membrane potential

4.

cotransport

13.

turgor

pressure5. water potential 14. plasmolysis6. osmosis7. solute concentration8. aquaporins9. tonoplast

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I. INTRODUCTION

1. Most water and mineral absorption occurs in the cells at the tips of the roots

(Cellular Level).

2.

Root hairs

are modified epidermal cells that are specialized for water absorption.They absorb soil solution which consists of water molecules and dissolved mineral ions that are not bound tightly to soil particles.

3. Soil solution flows through the hydrophilic walls of epidermal cells and travels along the cell walls and the intercellular spaces into the root cortex

4. Movement of soil solution into the cell involves: osmosis, diffusion, active transport, proton pumps,

aquaporins, water potential, cotransport, transport proteins4

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II. THREE

MAJOR PATHWAYS OF TRANSPORT IN PLANTS

A.

Transport is also regulated by the

compartmental structure of plant cells

B. The

plasma membrane

directly controls the traffic of molecules into and out of the protoplast

The plasma membrane is a barrier between two major compartments, the cell wall and the cytosolC. The third major compartment in most mature plant cells is the vacuole, a large organelle that occupies as much as 90% or more of the protoplast’s volume The vacuolar membrane regulates transport between the cytosol and the vacuole5

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Cell Compartments:

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Cell wall

Cytosol

Vacuole

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D.

In most plant tissues, the cell wall and cytosol are continuous from cell to cellThe cytoplasmic continuum is called the

symplast

The cytoplasm of neighboring cells is connected by channels called

plasmodesmata

The

apoplast

is the continuum of cell walls and extracellular spaces7

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E. Water and minerals can travel through a plant root by three routes: (Short-Distance Transport)

Transmembrane

route

: out of one cell, across a cell wall, and into another cell

Symplastic

route

: via the continuum of cytosol

Apoplastic

route: via the cell walls and extracellular spacesF. Long-Distance Transport at the Whole Plant LevelInvolves movement along the vertical axis (up and down)Involves bulk flow which is the movement of fluid in xylem and phloem driven by pressure differences at opposite ends of xylem vessels and sieve tubes8

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Transport Routes Between Cells

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III. ABSORPTION OF WATER AND MINERALS BY ROOTS

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1. Pathway:

Epidermis

 cortex endodermis stele (xylem)

2.

Mineral ions enter epidermal cells by

diffusion

and active transport using carrier proteins3. Movement usually a combination of apoplastic and symplastic routes4. Only minerals and water using the symplastic route move directly into xylem5. Minerals and water using apoplastic route are blocked at the endodermis by the Casparian strip and must enter an endodermal cell to move into xylem

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Lateral Transport

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6.

Mycorrhiza

hyphae

absorb water and selected minerals and can enable older regions of the roots to supply water and minerals to the plants.

Mycorrhiza

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IV. TRANSPORT OF XYLEM SAP

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A. Ascent of xylem sap depends on:

1. transpiration

-the loss of water vapor from leaves and other aerial parts of the plant

2. physical properties of water

-cohesion and adhesion

B. Xylem sap

(composed of water and minerals) flows upward against gravityC. Xylem vessels are close to each leaf cellD. Water must move upward to replace that lost by transpiration E. Pushing Xylem Sap: Root PressureUsually occurs at night when transpiration is lowRoot pressure (upward push of xylem sap) is generated by accumulation of minerals in stele which lowers the water potential and forces fluid up the xylem

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3. More water entering leaves than is transpired can result in

guttation

(discharge of water droplets at the leaf margin)

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F. Pulling Xylem Sap: The Transpiration-Cohesion-Tension Mechanism

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1. Water vapor in the airspaces of a leaf diffuses down its water potential gradient and exits the leaf via stomata by transpiration.

2. Transpiration produces negative pressure in the leaf, which exerts a pulling force on water in the xylem, pulling water into the leaf

3. Involves:

Cohesion

Adhesion

Tension (Negative Pressure)

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TRANSPIRATION

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4.

Cavitation

—formation of water vapor pockets in xylem that breaks the chain of water molecules and the pull is stopped

Once the water chain is broken the xylem vessels is no longer functional

Can occur during drought stress or freezing

5. Ascent of xylem sap is ultimately solar powered.

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Ascent of Xylem Sap

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V. CONTROL OF TRANSPIRATION

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A. Introduction

1. About 95% of water taken in is lost by transpiration through the stomata

2. Amount of water lost by a leaf depends on the number of stomata and the average size of the opening

3.

Guard cells

, by controlling the size of stomata, help conserve water

4. Benefits of transpiration: a. Assists in mineral transfer from roots to shoots b. Reduces leaf temperatures5. If transpiration exceeds delivery of water by xylem, plant wilts.6. Rate of transpiration is greatest on a sunny, warm, dry, and windy day

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7. Stomata are more concentrated on bottom of leaf away from the sun to reduce evaporation

8. Waxy cuticle also prevents water loss

B. How Stomata Open and Close

1. Guard Cells control stomatal diameter by changing shape.

Turgid Flaccid

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Turgid Guard Cells Flaccid Guard Cells

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2. When guard cells take in water, they become

turgid

and the gap between cells increases.

3. When guard cells lose water, they become

flaccid

and the gap between cells decreases.

4. Changes in

turgor pressure results primarily from the reversible uptake and loss of K+ by guard cellsStomata open—guard cells accumulate K+ and gain waterStomata closed—guard cells lose K+ and lose water5. Generally, stomata are opened during the day and closed at night.

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Cells Turgid/Stoma Open Cells Flaccid/Stoma Closed

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Cells Turgid/Stoma Open Cells Flaccid/Stoma Closed

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6. Stoma open at dawn because:

Light stimulates guard cells to accumulate K

+

and become turgid

Decrease of CO

2

because of PS

Internal clock of guard cells (circadian rhythms—cycles that have intervals of approximately 24 hours)7. Stoma close during daytime because of:Water deficiency (environmental stress)Production of abscisic acid

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C. Adaptations to Reduce Transpiration

1.

Xerophytes

—plants adapted to arid climates

2. Modifications of xerophytes:

small, thick leaves

thick cuticle

store water in fleshy stems during rainy seasonstomata concentrated on lower leaf surfacestomata may be located in depressionsCAM pathway for PS

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VI. TRANSPORT IN PHLOEM

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A. Introduction

1. Translocation

—transport of organic products of PS by phloem throughout the plant

-in angiosperms it involves sieve-tube members

-direction varies

2. Phloem

sap—aqueous solution that is mostly sucrose 3. Sugar source—an organ that is a net producer of sugar such as mature leaves 4. Sugar sink—an organ that is a net consumer or storer of sugar, such as a tuber or bulb

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B. Movement of phloem sap

Phloem sap moves from source to sink

Direction of flow depends on location of sugar source and sugar sink which depends on the season

Sugar is first loaded into sieve-tube members before moving to the sink.

4. Can involve:

Symplastic

and/or

apoplastic routesTransfer cells—modified companion cells with structures which increase cells’ surface area and enhance transfer between apoplast and symplastActive transport and cotransport

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Loading of Sucrose into Phloem

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5. Sugar will diffuse from phloem to the sink.

6. Water follows by osmosis.

C. Pressure Flow (Bulk Flow) of Phloem Sap in Angiosperms

1. Phloem sap moves by

bulk flow

driven by positive pressure (pressure flow)

2. Higher levels of sugar at the source lowers the water potential and causes water to flow into the tube 3. Xylem recycles the water from sink to source.

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Pressure Flow in a Sieve Tube

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