PDF-PlantPhysiol.(1991)96,675-6790032-0889/91/96/0675/05/$01.00/0Receivedf

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PlantPhysiolVol961991formsupportingauxiliarycellsandglandsasinthecaseofCannabisseeMahlbergetal22SomerecentworkpromisestoshedlightonthemoleculargeneticbasisoftrichomedevelopmentMutantsofArabid

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PlantPhysiol.(1991)96,675-6790032-0889/91/96/0675/05/$01.00/0Receivedf: Transcript


PlantPhysiolVol961991formsupportingauxiliarycellsandglandsasinthecaseofCannabisseeMahlbergetal22SomerecentworkpromisestoshedlightonthemoleculargeneticbasisoftrichomedevelopmentMutantsofArabid. 9+',"&:&))%"&(/#$)"&'==%"/&:&9+',"9+',"&:&&#x 0.1;))%"/$+,"&!+) A6""2&3*2$2*&=#'"1.+22"#"D&!*#(&*(&2-"&!*KA$+2R"D&A)'($,-.+22"#"D&3"$1L"0#'"D&."$(1*11"D&L*8$'("&A$%$DS"22+,":&*8$2*&;&3',/%"&!+) @1 United States District Court,D. Colorado.POD *Correspondingauthor.E-mailaddress:buts@gype.ucl.ac.be(J.-P.Buts).0889-8553/05/$ GIRIDHARANDTHIMANNactonacid-denaturedhemoglobin(SigmaChemicalCo.).Thereactionmediumfortheacidproteasecontained1.25mlof0.05Msodiumacetatebuffer(pH4.1),0.25mlof4%hemoglobinsuspension,and0.25mlofthesuper TEMPERATUREEFFECTSONRICINUSENZYMESratioof2mlto25gfreshweightoforiginaltissueandthesuspensionlayeredovera30to60%(w/w)linearsucrosegradientcontainingthesameadditivesasabove.Aftercentri-fugingfor2hrat27, PlantPhysiol.Vol.100,1992at40Cindarkness,andthenfor30to32hat200Cunderfluorescentlights(100gmolm-2S-1photonflux).Atthispointtheseedshadnotyetvisiblygerminated.Theseedswererinsedwithfour10-mLchangesofwa OSMOTICEFFECTSONCELLSbuffersolutionsat2Candthenboiledfor20minin80%meth-anol.Inleakageexperimentsthemaizeroottissuesweretreatedwith10mm14Cglucose,at25Cfor20min.Theywerethentransferredintobuffersolution Too?, and Betty and Coretta. Yoba starred in the DavidE. Talbert play His Woman, His Wife in 2000, touringthe country with the production. In 2011, he beganteaching a course entitled Proc.Natl.Acad.Sci.USA93(1996)9971AstandardcurveforAsAtotalwasgeneratedwithpurifiedAsA(Sigma),withtheadditionofdithiothreitoltoafinalconcen-trationof4mM.Dithiothreitolwasalsoaddedtothesamplesforthesta 6838PlantBiology:Staswicketal.2520EE154-Pcm-100050o-8-7-6-5-4Log1oMeJAconcentration(M)FIG.1.GrowthinhibitionofprimaryrootsbyMeJA.Rootlengthofjarlandwild-type(wt)plantswasmeasured9daysafterplanting.Bar Too?, and Betty and Coretta. Yoba starred in the DavidE. Talbert play His Woman, His Wife in 2000, touringthe country with the production. In 2011, he beganteaching a course entitled I[H[Robertsonetal[:Everydayattentionalfailures646sustainedauditorydiscrimination[TheslowingofresponsetimesfollowingerrorsalsoBrainResearch054061\0881[mayarisebecausesubjectsadoptamoreconservative7 TorreyMesaResearchInstitute,Syngenta,3115Mer-ryÞeldRow,SanDiego,CA92121,USA(www.tmri.BryanCollege,Dayton,TN37321,USA.partmentofBiologicalSciences,NorthernIllinoisUni-versity,DeKalb,IL60115,USA.Depart Downloaded from http://rupress.org/jcb/article-pdf/93/1/5/1075817/5.pdf by guest on 08 September 2022 Downloaded from http://rupress.org/jcb/article-pdf/93/1/5/1075817/5.pdf by guest on 08 September 2

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