Mohammed MBChB FIBMS Patholoy micoimmunopath University of Mustanisiriya College of Medicine Third stage Anaerobic Bacterial Infection Overview Anaerobic bacteria Bacteria that do not use ID: 1047510
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1. Asst.Prof.Dr. Nadia Hameed MohammedMBChB FIBMS Patholoy/mico-immunopathUniversity of MustanisiriyaCollege of Medicine Third stage Anaerobic Bacterial Infection
2. Overview Anaerobic bacteria: Bacteria that do not use oxygen for growth and metabolism but obtain their energy from fermentation reactions. These bacteria could be Obligate anaerobes or Moderately obligate anaerobes Obligate anaerobes usually lack super oxide dismutase (SOD) and catalase and are susceptible to the lethal effects of oxygenModerately obligate anaerobes have small amounts of both catalase and SOD and most anaerobic infections of humans are caused by this type.Facultative anaerobes: Bacteria that can grow either oxidatively, using oxygen as a terminal electron acceptor, or anaerobically, using fermentation reactions to obtain energy. Facultative anaerobes are common pathogens. Streptococcus species and the Enterobacteriaceae (eg, Escherichia coli ) are among the many facultative anaerobes that cause disease.
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4. Anaerobic Bacterial InfectionCaused by anaerobic bacteria wic are found as part of the normal microbiota throughout the human body and in high concentrations in the mouth and gastrointestinal tract.Infections caused by anaerobic bacteria are common. It is usually polymicrobial (anaerobes, facultative anaerobes, and aerobes), but anaerobes & facultative anaerobes are the common.Infection results when anaerobes and other bacteria of the normal microbiota contaminate normally sterile body sites.The main clinicopathological form of anaerobic infection is abscess formation.
5. Dental abscessSourcehttps://conciergedentalgroup.com/blog/understanding-dental-abscess-with-concierge-dental-group/ Sourcehttps://stock.adobe.com/search?k=abscess Dermal abscess
6. Anaerobic bacteria found in human infectionI / Gram negative anaerobes:Gram Negative BacilliBacteroides sppVery important anaerobes that cause human infection. They are a large group of bile-resistant, non–spore-forming, slender gram negative rods that inhabitant bowel and other sites.Bacteroides species are most often implicated in intra-abdominal infections and pelvic inflammatory disease &tubo-ovarian abscess.B fragilis is the commonest cause of human anaerobic infection.
7. Gram Negative Anaerobes2. PrevotellaP. Melaninogenica associted with pulmonary abscess & empyaema. P. Bivia. Associated wit pelvic inflammatory disease PID &tubo-ovarian abscess3. PorphyromonasThey are normal oral microbiota & may occur at other anatomical sitesPorphyromonas species can be cultured from gingival and periapical tooth infections
8. Gram Negative Anaerobes4. Fusobacteriamost human infections are caused by Fusobacterium necrophorum and Fusobacterium nucleatumF. necrophorum is quite virulent, causing severe infections of the head and neck that can progress to a complicated infection called Lemierre’s disease (uncommon complicated bacterial pharyngitis/tonsillitis and involves an extension of the infection into the lateral pharyngeal spaces of the neck with subsequent septic thrombophlebitis of the internal jugular vein).F. nucleatum is a significant component of the gingival microbiota as well as the genital, gastrointestinal, and upper respiratory tracts. As such, it is encountered in a variety of clinical infections such as pleuropulmonary infections, obstetric infections, significantly chorioamnionitis, and complicating periodontal disease.
9. Gram Negative Anaerobes5. Bacteria that cause bacterial vaginosis:Bacterial vaginosis is a common vaginal condition of women of reproductive age.So named because inflammatory cells are not present.Two gram –ve anaerobic bacilli, Gardnerella vaginalis and Mobiluncus species, have been most specifically associated with the disease process.Vaginosis diagnosed by : “fishy” odor vaginal discharge, presence of ‘clue cells,” (vaginal epithelial cells covered with many gram-ve bacilli ) in wet smear, absence of inflammatory cells & absence of other common causes of vaginitis such as trichomonads or yeasts.In pregnant women, it is associated with premature rupture of membranes and preterm labor .
10. Clue cells/ Bacterial Vaginosis(Gram stain of wet vaginal swab)Sourcehttps://www.researchgate.net/figure/Wet-mount-appearance-of-clue-cells_fig1_340179493
11. Gram Negative AnaerobesB. Gram negative cocciVeillonella species are a group of small, anaerobic, gram negative cocci that are part of the normal flora of the mouth, the nasopharynx, and probably the intestine.Occasionally isolated in polymicrobial anaerobic infections,
12. Gram Positive AnaerobesII/ Gram positive anaerobesGram-Positive BacilliActinomyces:G+ve, non motile, non spore forming, obligate anaerobic filamentous bacteria.They are normal flora of mouth and intestine.Actinomyces israelii cause actinomycosis in humanActinomyces bovis cause disease in cattle (lumpy jaw)Actinomyceshttps://phil.cdc.gov/Details.aspx?pid=22293
13. Gram Positive AnaerobesActinimycosis Aactinimycosis is a chronic subcutaneous granulomatous infection in which sinuses are formed & open on the surface of skin.Discharging pus containing small yellowish granules (sulphur granules) which are compact colonies of actinomyces.The jaw and cervicofacial region are the usual site of infection often following tooth extraction, however abdominal or thoracic infection can occur. Source of the image : https://www.researchgate.net/figure/Swelling-of-the-cheek-in-a-patient-with-actinomycosis-Note-the-possible-beginning-of-a_fig1_236976734 ActinomycosisSulphur granules
14. Gram Positive Anaerobes2. Clostridea spp in separated lecture3. LactobacillusLactobacillus species are major members of the normal microbiota of the vagina. Inhibit yeast over growth by lactic acid production from their metabolism helps maintain the low pH of the normal adult female genital tract.4. Propionibacteriummembers of the normal microbiota of the skin, oral cavity, large intestine, conjunctiva, and external ear canal. On Gram stain, they are highly pleomorphic, showing curved, clubbed, or pointed ends; long forms with beaded uneven staining; and occasionally coccoid or spherical forms.Their metabolic products include propionic acid, from which the genus name derives.
15. Gram Positive AnaerobesPropionibacterium acnes, often considered an opportunistic pathogen, causes the disease acne vulgaris.It causes acne by producing lipases that split free fatty acids off from skin lipids. These fatty acids can produce tissue inflammation that contributes to acne formation.In addition, P acnes is frequently a cause of postsurgical wound infections, particularly those that involve insertion of devices.
16. https://link.springer.com/chapter/10.1007/978-3-319-67651-7_8 Propionibacterium acnes
17. Gram Positive Anaerobes5. Eubacterium, Eggerthella, Bifidobacterium, and ArachniaAnaerobic, pleomorphic, gram-positive rods. Found in mixed infections associated with oropharyngeal or bowel flora.B. Gram-Positive Coccinormal microbiota of the skin, oral cavity, upper respiratory tract, gastrointestinaltract, and female genitourinary system.Peptostreptococcs is the important group.These organisms have been associated with serious infections such as brain abscesses, pleuropulmonary infections, necrotizing fasciitis, and other deep skin and soft tissue infections, intra-abdominal infections, and infections of the female genital tract.
18. PATHOGENESIS OF ANAEROBIC INFECTIONSInfections most often are mixed infectionAnaerobic infection is usually mixed with facultative anaerobic bacteria (e.g. E. Coli) as the later consuming the oxygen and thereby produce an environment in which Bacteroides species and other anaerobes can grow.Many anaerobic bacteria produce heparinase, collagenase, and other enzymes that damage or destroy tissue.The capsular polysaccharides of Bacteroides are important virulence factors.Bacteroides species have lipopolysaccharides (endotoxins) but much less toxic than those of other gram-negative bacteria.B fragilis produces a SOD and can survive in the presence of oxygen for days.
19. DiagnosisClinical signs suggesting possible infection with anaerobes include the following:1. Foul-smelling discharge (caused by short-chain fatty acid products of anaerobic metabolism)2. Infection in proximity to a mucosal surface (anaerobes are part of the normal microbiota)3. Gas in tissues (production of CO2 and H2)4. Negative aerobic culture results
20. TreatmentTreatment of mixed anaerobic infections is by surgical drainage (under most circumstances) plus antimicrobial therapy.The most active drugs for treatment of anaerobic infections are clindamycin and metronidazole.Alternative drugs include cefoxitin, cefotetan, some of the other newer cephalosporins, and piperacillin, but these drugs are not as active as clindamycin and metronidazole.Penicillin G remains the drug of choice for treatment of anaerobic infections that do not involve β-lactamase–producing Bacteroides and Prevotella species.
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22. Common locations of infections involving anaerobic bacteria (Image source: Koneman, Ref-1)
23. Reidle, S., Morse, S. A., Meitzner, T., and Miller, S. 2019. Jawetz, Melnick & Adelberg’s Medical Microbiology , Twenty-Eight Edition. The McGraw-Hill education, Inc. USACentre for Disease Control and Prevention Public Health Image Library (PHIL).Levinson, W. 2016. Review of medical microbiology and immunology,14th edition. The McGraw-Hill Companies, Inc. USAKumar, S. 2012. Textbook of microbiology. Jaypee Brother Medical Publishers (P) Ltd. New Delhi, India. https://conciergedentalgroup.com/blog/understanding-dental-abscess-with-concierge-dental-group/ https://stock.adobe.com/search?k=abscess https://www.researchgate.net/figure/Wet-mount-appearance-of-clue-cells_fig1_340179493 https://www.researchgate.net/figure/Swelling-of-the-cheek-in-a-patient-with-actinomycosis-Note-the-possible-beginning-of-a_fig1_236976734
24. Thank you