PPT-THE OPTICAL (LIGHT) MICROSCOPE

It is the most important tool to study of microstructure despite the fact that more sophisticated electron metallographic instruments such as scanning electron microscopy SEM and transmission electron microscopy TEM have been evolved. Microscopes are delicate and expensive instruments they should be handled with utmost care Before you use the microscope your instructor will explain its proper use Following are rule s that will protect the microscope and insure that you can make m POLARISED LIGHT MICROSCOPE . Designed to observe optically anisotropic specimen (BIREFRINGENCE SPECIMEN). Equipped with POLARISER and ANALYZER . Works on the principle of INTERFERENCE . COMPONENTS OF POLARISED . CONCEPTS EXPLORED IN THIS LESSON. ocular lens / eyepiece. body tube. arm. base. light source/illuminato. r. revolving/rotating nosepiece. objective lenses. coarse adjustment knob. fine adjustment knob. How to Focus on Specimen. Turn on light source on high. Turn the objective to low (4x). Lower the objective lens so that it is close but not touching the slide. Center the image by moving the stage. Adjust the diaphragm for optimum light. Light Microscope. Simple – uses a single lens. Compound – uses a set of lenses or lens systems. Simple Light Microscope. Compound Microscope. Mechanical Parts. Used to support and adjust the parts. Compound Light Microscope. The compound light microscope is a common tool in a life science laboratory. A . compound light microscope. is an instrument that magnifies small objects so that they can be seen easily. . Microscope Review The diagram represents a cell in the field of view of a compound light microscope. In which direction should the slide be moved on the microscope stage to center the cell in the field of view XP-100 XP100 is extremely useful for Iatrologyand and industry fields. This microscope is good at identifying crystals suspended in liquid, identifying minerals in core samples, detecting defects in . Function of microscope. :. Magnification: . to . magnify (enlarge) . the object being examined.. Resolution: . its ability to differentiate two objects when you view them on a specimen slide.. Working distance: . Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid M I C R O S C O P E CONTENTS: • WHAT ? • HISTORY • CLASSIFICATION OF MICROSCOPE • SIMPLE MICROSCOPE • COMPOUND MICROSCOPE • PARTS OF COMPOUND MICROSCOPE What Is A Microscope ? • A mic A perture is the main lens or mirror that gathers the light to a F/ number is a measure of the speed and clarity of the optical – An astronomer wants to design a telescope that takes up the The elementary particle that defines light is the photon.. There are 3 basic dimensions of light. Intensity (amplitude) which is related to the perception of brightness. Frequency (wavelength), perceived as colour. th. Grade. The History. Many people experimented with making microscopes. Was the microscope originally made by accident? (Most people were creating telescopes). The first microscope was 6 feet long!!!.