Physical FormSupplied as a solution in buffer aboveExpression SystemPurityo ww of 11000 to 1200should provide an effective range for over 99 cleavageof most target proteins The optimal ratio and clea ID: 878795
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1 4-611 Bowen Science BuildingIowa City, I
4-611 Bowen Science BuildingIowa City, IA, 52242Tel: 319-335-7671E-mail: pcf@healthcare.uiowa.eduWebsite: medicine.uiowa.edu/crystallography Physical Form:Supplied as a solution in buffer above.Expression System:Purity: o (w/w) of 1:1000 to 1:200should provide an effective range for over 99% cleavageof most target proteins. The optimal ratio and cleavagetemperature should be determined empirically. A goodstarting point is a ratio of 1:200 (1 ug SUMO protease for200 ug of fusion protein) incubated for 4 hours at 4 °C�for 99% cleavage. SUMO protease, as supplied, canbe directly added to the fusion protein. Actual cleavageefficiency depends on the spacing between the cut siteand the target protein.Cleavage ProtocolThis product is suitable for laboratory research use only