PPT-Nucleosomes : what, why and where?

Rob Brewster Outline What is a nucleosome how is DNA packagedorganized in Eukaryotes Why do nucleosomes form DNA is stiff how do 100 bp loops form so readily Where do . : what, why and where?. Rob Brewster. Outline. What is a . nucleosome. ?. - how is DNA packaged/organized in Eukaryotes?. Why do . nucleosomes. form?. . - DNA is stiff, how do ~100 . bp. loops form so readily?. May 2010. Nucleosome. Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . (Hopefully). By. And . KochLab. This presentation will include . amazing topics like:. The Latest in Tweezers Technology. Biological Advancements. And more…. Thank You!. KochLab. Guys. Steve Koch – Lab Don. Part 2. DNA . Topology. Some of the following slides and text are taken from the DNA Topology lecture from Doug . Brutlag’s. January 7, 2000 Biochemistry 201 Advanced Molecular Biology Course at Stanford University. (Hopefully). By. And . KochLab. This presentation will include . amazing topics like:. The Latest in Tweezers Technology. Biological Advancements. And more…. Thank You!. KochLab. Guys. Steve Koch – Lab Don. DNA Packaging—Why and How. If the DNA in a typical human cell were stretched out, what length would it be? What is the diameter of the nucleus in which human DNA must be packaged?. What degree of DNA packaging corresponds with “diffuse DNA” associated with G1? What kind of DNA packaging is associated with M-phase (“condensed DNA”)?. The formation of a highly organized DNA-protein complex, termed as chromatin, which is a nucleoprotein complex completes the packing.. Chromatin can be defined as highly condensed chromosomes at metaphase stage, and very diffuse structures in course of . By. Dr. . Walaa. . Bayoumie. El . Gazzar. Chromatin. The simplest form of chromatin is . present . in non dividing eukaryotic cells, when chromosomes are not sufficiently condensed to be visible by light microscope.. 3D . genome organisation. Vladimir Teif. BS222 – Genome Science Lecture 6. Module structure. Genomes, sequencing projects and genomic databases (VT. ). Sequencing . technologies (VT. ). What is a gene? . Chromatin-binding factorshistone macromolecules that bind either directly or indirectly to DNA.Transcription factorhistone protein that directly binds to DNA.Architectural proteinsProteins that have a Chapter 4 - Genes, Genomes, and DNA. Figure 4.01. Neutrophil Extracellular Traps (NETs). Contact with bacteria, . protozoa. , yeast, or . filamentous fungi. (hyphae), or their products (e.g., bacterial lipopolysaccharide) stimulates neutrophils. This causes (A) the . Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . Nucleosomes. Don and Ada . Olins. in 1974. , and their existence and structure (as . histone . octamers. surrounded by approximately 200 base pairs of DNA. ) were proposed by . Roger Kornberg. .. . The role of the nucleosome as a . Chromatin organization marks . exon-intron. structure. [Schwartz S, Meshorer E, Ast G . Nat . Struct. Mol . Biol. , 2009 Sep;16(9):990-6]. . Preface:. Crystal structure of . nucleosome. core particle.