Fibrino g en CONTENTS Determination of fibrinogen time For diagnostic use only When diluted plasma is clotted with excess of thrombin the fibrinogen level is inversely propo REAGENT COMPOSITION ID: 955002
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Fibrino g en CONTENTS CLAUSS METHOD Determination of fibrinogen time For diagnostic use only When diluted plasma is clotted with excess of thrombin, the fibrinogen level is inversely propo REAGENT COMPOSITION Fibrinogen 100 NIH U/mL in a buffer, Imidazole Imidazole buffer , stabilizers and preservative. Optative. Plasma Control Level 1 Ref. 3520101 Draw venous blood into a plastic or siliconized syringe Immediately transfer 9.0 mL of blood into a tube containing 1.0 mL of 3.2% o
r 3.8% sodium citrate solution. or Draw venous blood into a commercial vacuum tube containing 3.2% or 3.8% sodium citrate solution. Insure that a full draw has been obtained since the ratio of 9 parts blood to 1 part citrate is critical. A heparinized lock or transfer line should not be used. It is generally recommended that the second or third tube draw be used for coagulation tests. 2. Plasma Preparation Mix well by inversion and centrifuge at 2,500 x g for 15 minutes soon after blood collection. Unless samples are to be processed immediately, transfer the plasma into a plastic tube. Plasma that is clearly hemoly
zed or contains� 10,000 platelets per mL or red cells is not suitable for coagulation testing. 3. Plasma Storage Plasma samples may be stored at room temperature (18 to General laboratory equipment. PROCEDURE This procedure is far to manual or semi-automated coagulation systems. Follow the instructions of the instrument used. For best results, duplicate samples are recommended. 1. 2. Bring the sufficient volume of reagent to room temperature. Prepare 1:10 dilution of the plasma (or plasma control) with Imidazol buffer (i..e. 25 3. 4. 5. 6. of the diluted plasma to the tube test