Measuring Length 1 Meter stick Ruler Each marked line represents 1 cm The smaller lines between cm are mm There are 10 mm in 1 cm There are 100 cm in 1 m B Measuring Volume Graduated Cylinder ID: 1045411
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1. Tools of the Biologist
2. In order to observe, discover, and explore, scientists use many different pieces of lab equipment.Measuring Length1. Meter stick, Ruler Each marked line represents 1 cm.The smaller lines between cm are mm.There are 10 mm in 1 cm There are 100 cm in 1 m
3. B. Measuring VolumeGraduated CylinderUsed to measure exact volumes To Use:Fill to the appropriate level.Note that liquid in a glass graduate cylinder curves upward at the edges. This is called the MENISCUS.Read the volume at the bottom of the meniscus when looking at eye level.
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5. 12 mL16 mL14 mL14 mLPay attention to the scale of the graduate cylinder(How much the lines go up by)
6. B. Measuring Volume2. BeakerUsed to measure a approximate volumeTo use: fill with the appropriate volumeThere will not be much of a meniscusRead at eye level.
7. B. Measuring Volume 3. Erlenmeyer and Florence FlasksUsed to measure an approximate volumeAnd/or to mix solutions To use: same as beakerErlenmeyerFlorence
8. B. Measuring Volume 4. Pipette Used to measure an approximate volumeMeasures one drop at a timeTo use: (to fill) submerge, gently squeeze and release(to release) carefully squeeze 1 drop at a time
9. C. Measuring Mass4. Electronic BalanceUsed to measure an exact massTo use: Turn on, zero the balance, set to correct unit, carefully place sample on tray. If sample is placed in a container subtract the mass of the container from the total mass
10. D. Tools for Dissection1. Dissecting PanUsed to hold specimens in placeTo use: Lay the specimen on the tray and pin to the rubber bottom
11. D. Tools for Dissection2. Dissecting Needle (Probe)Multi-purpose tools to move parts of the specimen
12. D. Tools for Dissection3. ScalpelUse: to make precision cuts and/or take samples
13. D. Tools for Dissection4. ForcepsUse: to grasp and manipulate parts of a specimen
14. E. Using Chemicals1. Test Tube/Test tube rackUse: to carry out chemical reactionsWhen not in use, put in test tube rack
15. E. Using Chemicals1. Test Tube HolderUse: to hold and move test tubesSqueeze handle so the clamp opensRelease handles so the clamp closes around test tube
16. E. Using Chemicals1. Test Tube BrushUse: cleans inside of test tube
17. F. Observing Specimens and Samples1. Petri DishUse: small dish to hold samples and/or grow bacterial samples.
18. G. Safety Equipment1. Safety GogglesUse: Place over your eyes for protection
19. G. Safety Equipment1. Safety GlovesUse: Place over your hands for protection from heat, chemicals, body fluids, etc
20. G. Observation1. Compound Light MicroscopeUse: enlargement of microscopic specimens
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22. 1. Compound Light MicroscopeMagnifies specimen 100s of times“Compound” means it has several lenses2. How to Use a MicroscopeAlways carry the microscope by the arm while supporting the base Position the microscope so the arm is near to youALWAYS START WITH THE LOWEST POWER MAGNIFICATION
23. 2. How to Use a Microscope (cont)Center the slide on the stageCenter the specimen in the field of view (remember the image is inverted)Use the coarse adjustment to focus the slide - Once the slide is in focus, switch to a higher power then refocus with fine adjustmentONLY USE THE FINE ADJUSTEMENT ONCE YOU’VE SWITCHED TO THE HIGH POWER LENS – IF NOT THE SLIDE WILL CRACK
24. Making a Wet Mount SlideWet mounts are microscope slides that contain a specimen mounted in water. A cover slip (or cover glass) is used to flatten the specimen and the water drop. Obtain a clean microscope slide and cover slip. Hold cover slips only by the edges.Add a small drop of water to the slide.Place the specimen in the drop of water. Hold the cover slip at a 45˚ angle next to the drop of water, slide it over until it hits the water. Place it onto the water. This helps prevent air bubbles.
25. Adding a Stain/Chemical to Wet Mount SlideStains: Chemicals added to a slide in order to see parts of a specimen more clearly.
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27. Adding a Stain/Chemical to Wet Mount SlideStains: Chemicals added to a slide in order to see parts of a specimen more clearly. After making a wet mount slide, place a drop of stain on one side of the cover slipGently touch the other side of the cover slip with a piece of paper towelThe paper towel will “pull” the stain under the coverslip
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29. Human Red Blood Cells
30. Plant CellsFly HeadSperm CellsPollen
31. Cancer CellsWhooping Cough E Coli
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