AAALAC International is a voluntary accrediting organization that enhances the quality of research teaching and testing by promoting humane responsible animal care and use It provides advice and independent assessments to participating institutions and accredits those that meet or exceed appl ID: 775996
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Slide1
Slide2What is AAALAC International?
AAALAC International is a voluntary accrediting organization that enhances the quality of research, teaching, and testing by promoting humane, responsible animal care and use. It provides advice and independent assessments to participating institutions and accredits those that meet or exceed applicable
standards
.
Slide3Accreditation
Is
completely
voluntary
and
confidential
.
Is performance-based.
Is a peer-review process.
Slide4Three Primary Standards Used to Evaluate Programs
Guide for the Care and Use of Laboratory Animals (NRC 2011)Guide for the Care and Use of Agricultural Animals in Research and Teaching European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, Council of Europe;(ETS 123)
Slide5The Process
AAALAC accredits
the
entire “program” which includes:
animal care and use policies and responsibilities
animal environment, housing and management
veterinary medical care
physical plant
EH&S
Occupational Health and Safety
Slide6Maintaining standards
of animal care and use
Slide7Protocol ReviewGrant Application Review (GAR) from funding sources (NIH, NSF and AHA)Program ReviewFacilities and Use Area ReviewTo address animal concerns
Role of the IACUC
Slide8Grant Application Reviews
(GARs)
* Encompasses NIH, AHA, & NSF awards
* Comparison of proposed live vertebrate animal work
with approved IACUC protocol(s)
* GAR form is submitted
via
the forms tab on the
IACUC website
1) Research Design & Methods
2) Vertebrate Animals section
3) Off-site & Collaborators’ materials
Slide9Stumbling Blocks
Submitting a GAR on or after the award dateHaving pending protocols & modsProviding out-dated or incorrect materialsFailure to provide collaborators’ materials
GAR letter IACUC protocol approval letter Regulatory acceptance letter
Slide10IACUC Protocol
Know your animal protocol
.
Perform the study as described in your protocol.
Make
no deviations from the protocol
.
There is no such thing as a pilot study that does not need IACUC approval
.
Slide11Recent Changes to Protocol Review Process
No longer required: Listing every transgenic rodent strain to be used or bred on your protocol. List only background strains.
The
practice of extending a protocol’s life to the end of the month in which it was initially approved
has
ended.
Protocol lifespan is now exactly 3 years, or 1095 days.
Reminder emails have been adjusted to accommodate this change.
Annual renewals must be approved before the exact one and two year marks from the date of initial approval.
Slide12Upfront with the IACUC
T
he IACUC Office can help you with issues like controlled drug storage, recordkeeping, protocol writing and laboratory practices.
We can also help prepare
you for laboratory inspections, and provide training on use of the Animal Research Online system to submit protocols.
We can come to your office or you can come to our suite.
Call our office (412-383-2008) to make arrangements.
Slide13Training Prior to Work With Animals
Training for research
staff and animal care staff
IACUC-required training includes RPF Modules, species specific training, and all required EHS training
Hands on training by PI or designated staff member
Documented
on-the-job training
Certification by AALAS is
encouraged
Slide14First
Impressions
Clearly represent that animal users are interested in a quality
program
Keep
laboratory neat and organized
This says a great deal about the
lab
Good
Animal Care = Good Science
Slide15Maintain housekeeping in animal use areas
Slide16Storing Equipment & Supplies
Slide17IACUC Required Signage
Animal
related injury procedures
Emergency vet care
Rodent Survival Surgery
Reporting Concerns (animal welfare
)
Emergency Evacuation Plan
Slide18Rodent Aseptic
Surgery
Must
follow
the University
of
Pittsburgh IACUC’s
published g
uidelines
S
terilization
of
instruments and supplies
Surgical preparation of animal and
surgeon
Anesthesia monitoring
An
anesthetized animal can not be left
unattended
Post-Operative Care consistent with
The IACUC Surgical
P
olicy
What is described
in your protocol
Slide19Inhalant Anesthesia Use anesthetics only as described in the protocol.Gas anesthetic vaporizers must be calibrated every 2 years. Gas anesthetic agents must be appropriately scavenged.Animals must not come in contact with liquid anesthetic agents such as isoflurane.
Slide20Documenting Procedures
Blue Procedure Cards
Research Records
Dates, procedures, observations, initials
Write legibly
Slide21Only use those drugs listed in the protocolDrugs must be pharmaceutical grade unless otherwise approved in protocolOther drugs that may be needed for clinical care must be under the direction of a veterinarianUse only in-date drugs and maintain a system for identifying and disposing of expired drugsFollow University of Pittsburgh Controlled Substances Guidelines.
DRUGS
Slide22Labeling Secondary Containers
Slide23Animal Transport
Use only
the
transport
routes approved in the protocol
Use only
approved
transport
containers
Refer to animal transport policy
Slide24Animal Health Issues?Post-procedure complications. What do you do? What do you do with a sick animal on the weekend?Do you know the point at which the animal should be euthanized? What is approved in the protocol?Remember: ALL clinical care of animals must be coordinated through the Division of Laboratory Animal Resources (DLAR).Do you know your DLAR facility assigned veterinarian?
Slide25DLAR Facility Veterinarians
Facility
Primary Veterinarian
Backup Veterinarian
BRDG2
Bob Wagner
Joe Newsome
BTC
Bob Wagner
Joe Newsome
BST
Amanda Fisher
Amy Cassano
BST3
Beth Ahner
Anita Trichel
HCC
Joe Newsome
Lora Rigatti
MIRM
Bob Wagner
Joe Newsome
PBRC
Anita Trichel
Erin Jackson
RBL
Anita Trichel
Beth Ahner
RRB
Erin Jackson
Anita Trichel
SBST
Amanda Fisher (8th & 10th floors); Amy Cassano (9th floor)
Amy Cassano (8th & 10th floors); Amanda Fisher (9th floor)
SRRC
Amy Cassano
Amanda Fisher
SNI
Julia Nyaundi
DLAR veterinarians
CLC
Amy Cassano
Amanda Fisher
LSA
Amy Cassano
Amanda Fisher
PLE
Amy Cassano
Amanda Fisher
Slide26Clinical Records for Non-Rodent MammalsEach animal must have an individual clinical record.A post-procedure form (http://www.iacuc.pitt.edu) must be completed for every procedure done on the animal, including euthanasia.For animals other than rodents, vital signs must be recorded at least every 15 minutes during surgery (including non-survival surgeries) on the anesthetic monitoring form (http://www.iacuc.pitt.edu )
Slide27Clinical Records for
Non-Rodent
Mammals
Clinical records are maintained in electronic format in CAMS
.
All drugs administered to an
animal by research personnel
must be documented on clinical record/procedure forms
.
Completed hard copy clinical record/procedure forms must be submitted to DLAR Veterinary Services to be scanned into CAMS.
Slide28Visitors Policy
Visitors to animal facilities must obtain permission from the Attending Veterinarian and complete any EH&S required clearances or training prior to entering the facility. To request permission, contact the DLAR at 412-648-8950 or email the Attending Veterinarian at dschab@pitt.edu.
Slide29PPE Requirements in Facilities
Follow the signs & wear PPE properly!
Slide30Slide31Animal Housing
Minimum cage space requirements are listed in the Guide, the Animal Welfare Act & Regulations, and summarized in DLAR SOP #112 (
Minimum Space Requirements of Primary Enclosures
) posted on the DLAR website.
Overcrowded mouse cages primarily result from
Overdue weaning (> 28 days)
Multiple litters in the same cage
Managing rodent breeding colony cage density is the PI’s responsibility.
Slide32Animal HousingA CAMS generated cage card must be on every cage with the bar code and any EH&S biohazard and/or chemical hazard sticker visible.Animals cannot be housed outside of the designated animal facility unless approved by the IACUC.
Slide33CAMS Cage Cards
Slide34Social Housing
AAALAC considers social (pair or group)
housing
the default method of housing for social species.
Single housing of social species must be done in accordance with the IACUC Social Housing Policy.
Scientific basis outlined in the approved protocol
Veterinary exemption for health/welfare concerns
Program wide exemptions (post-op, specific breeding colony situations, research attrition, aggression or social incompatibility, male rabbits)
http://
pre.iacuc.pitt.edu/policies
Slide35Identify Single Housed Animals
Rationale for single housing must be provided at the cage level.
Appropriate colored “dot” sticker should be placed on the CAMS card.
Slide36Sharps Safety
Sharps injuries are a significant injury and health hazard for researchers and animal care workersSharps include:Needles, scalpel blades, lancets, razor blades, and broken glass
Slide37Sharps Safety
Reducing the hazard from sharps requires the use of:
Engineering controls
Equipment designed to reduce risk
Work practice controls
Handling practices that reduce the risks of exposure
Slide38Making Sharps Use Safer
Engineering ControlsPuncture Resistant Sharps ContainersRequired for sharps disposalAvailable in immediate workspaceDo not recap needles for disposalSyringes should be empty for disposalUse of some chemicals may require a dedicated sharps container labeled for the chemical hazard.Do not over fill.
Slide39Making Sharps Use Safer
Engineering Controls-control the hazard by reducing/removing itSelf sheathing needlesMechanical recapping devicesShields
Slide40Safer Sharps Use
Work Practices Eliminate the use of sharps and glass whenever possibleUse safety engineered sharpsTrain users in proper sharps handling, and review accident proceduresHousekeepingMinimize “two handed” sharps techniques
Slide41One Handed Recapping
Slide42ChemicalsAll chemical containers must be labeledSee “IACUC Policy on Secondary Labeling” An expiration date must be on the label of all drug containersKeep chemicals away from human or animal food itemsFume hoods and Biological Safety Cabinets must be certified annually
Tamoxifen
Slide43Occupational Health and Safety
All animal users must be enrolled in the Animal Exposure Surveillance Program (AESP), and update information every 3 years.
http://www.ehs.pitt.edu/biological/animal.html
Slide44Occupational Health and Safety
Injuries and Exposure Response: Employees and Students should know the process!
Wash bite or exposed area with soap and water (or flush eyes and mouth with water)
Report the incident to your supervisor
Report for treatment to Employee Health (business hours) or UPMC Presbyterian Emergency Dept. (after
3:30
pm)
Complete reporting process to Workman’s Comp within 24 hours (1-800-633-1197)
Slide45Occupational Health and Safety
Hazard Assessment:EH&S risk assessment provides guidance on:Chemical, biological and physical risksEngineering ControlsPPE worn during procedures involving the agents or physical hazardTraining and Medical Surveillance Requirements
Slide46Occupational Health and Safety
To work on a project Animal Users should know:Standard procedures (ex. Decontamination)Hazards present in the animal room.What PPE to wear.How to respond to an exposure or animal bite.
Slide47IACUC Policy Updates and Revisions
Medical Record Keeping-RodentsSurgical PolicyTransportation Policy and GuidelinesSocial Housing Policy
University
of Pittsburgh
policies are designed to
maintain a
program
consistent with the
Guide
for the Care and Use of Animals
Slide48Training and Education Opportunities
Upfront with the IACUC
Webinars
Reproducibility of Animal Models
– AALAS –
Identifying
and Preventing Distress in Laboratory
Animals
– PRIM&R-
Animal
Rights Extremism: An Overview
–
NABR-
Physiological Effects of Animal Handling and Housing on Metabolic Imaging
Studies –
AALAS
Rescue strategies for lines of genetically engineered
mice -
AALAS
Gait Analysis in Lab Animals: Studying coordinated movement and associated
disorders –
Inside Scientific
Addressing breeding problems in mice: 40 days and still no
pups-
AALAS
Educational Resource
AALAS Learning Library
accounts are assigned to researchers through the IACUC Training coordinator :
cgubish@pitt.edu
Courses are offered which emphasize the appropriate handling, care and use of animals in research, which include a wide range of topics with many points of interest
Slide49www.iacuc.pitt.edu
www.dlar.pitt.edu
www.rcco.pitt.edu
www.ehs.pitt.edu
The Information Super
Highway
www.ibc.pitt.edu
http://cme.hs.pitt.edu
www.AAALAC.org
Slide50Please contact us if you have any questions
IACUC Office 412-383-2008 IACUC@pitt.eduDLAR Main Office 412-648-8950 DLAR@pitt.edu