HandsOn Lab Directions If you choose this option Read through and complete the procedures outlined in this Lab Report Once you have completed the lab you will turn in the results analysis and conclusion sections ID: 240864
Download Presentation The PPT/PDF document "4.03 Part 1" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
4.03 Part 1
Hands-On LabSlide2
Directions
If you choose this option: Read through and complete the procedures outlined in this Lab Report.
Once you have completed the lab, you will turn in the results, analysis, and conclusion sections.Slide3
Accident Summary
It wasn’t the first time Chris had seen the inside of an emergency room. He was surprised the nurses didn’t hand him a frequent visitor card when he stumbled in yet again with another injury. This time it was his leg. Blood was running down his thigh, and as tough as he was, he was starting to feel a little faint.
“I’d say it was nice to see you again but it seems inappropriate at the moment,” Dr. Taylor mused as he assessed Chris’s new injury. “You’ve really done it this time. Lucky for you these minor scrapes clotted quickly, but this large gash…it’s going to need stiches.”
“It just adds to my battle scars,” Chris announced with pride.
Dr. Taylor groaned. It was a typical Chris response. “It also adds to your medical bills, Chris,” Dr. Taylor responded as he turned to grab clean gauze from the supply cabinet. “When are you going to give up Parkour,” he asked concerned. “Free-running is hazardous to your health.”
Chris did not respond. He had fainted. He also looked pretty pale. Dr. Taylor surmised from the puddle of blood pooling around his leg that Chris had taken his time getting to the ER, and he’d lost too much blood along the way. He was going to need a blood transfusion.Slide4
Blood Samples
You are the nurse assisting Dr. Taylor. Chris needs a transfusion, but you will need to determine his blood type first. To reveal Chris’s blood type, his blood sample needs to be tested using anti-A serum, anti-B serum, and anti-Rh serum. Anti-serum contains antibodies against the blood type labeled. For example, anti-A serum contains antibodies that bond to the A antigens on red blood cells and causes them to clump.
Fill in the blank spaces in the chart to help you determine the blood type and the Rh factor (+ or -) of Chris’s blood sample. (Worth 8 points)
Each correct answer on the following slide will be worth 1 point.
For full credit…..Be sure to include both the letter (or letters) of your blood type, as well as the proper indicators showing it as positive or negative.Slide5
Reactions w/ Anti-A Serum
Reactions w/ Anti-B Serum
Reactions w/ Anti-Rh Serum
Blood Type
Clumping (+)
No Clumping (-)
Clumping
Clumping (+)
No Clumping (-)
No clumping
No Clumping (-)
Clumping (+)
Clumping
No Clumping (-)
Clumping (+)
No Clumping
Clumping (+)
Clumping (+)
Clumping
Clumping (+)
Clumping (+)
No Clumping
No Clumping (-)
No Clumping (-)
Clumping
No Clumping (-)
No Clumping (-)
No Clumping
Slide6
Lab Component
Making the blood sample and the anti-serumsSlide7
Materials Needed
1 cup milk
Toothpicks
Vinegar
Water
8 cups
1 dropper or teaspoon
2 bottles of food coloring – red and green (optional)
Marker and tape to label the blood sample and serums Slide8
Preparation
Mix 1 cup of milk with 1 cup of water.
Add enough red food coloring to get a bright red color and then add a few drop of green to make it a deeper red color so that it looks more like real blood.
Note: Making it look like blood is optional.
You
don’t need to make it red to test it
.
Pour half of the blood into a second cup. Label one cup “sample 1” and the other cup “sample 2.” Slide9
Anti A Anti B Anti-Rh
Vinegar Vinegar Water
You will need three anti-serums.
There should be an anti-A, an anti-B, and an anti-Rh serum.
You will only need ¼ cup of each serum made according to the chart.
These will be your 3 Anti Serums that are used during the rest of this hands-on lab activity
At this point, there should be 2 samples of Chris’s blood, an anti-A serum, an anti-B serum, and
an
anti-Rh serum.Slide10
Procedures to test Chris’s blood sample
Step 1: Place 10 drops (or 1 teaspoon) of sample 1 into 1 teaspoon of each serum.
Step 2: Use clean toothpicks to stir the blood into each serum for 30 seconds.
Step 3: Record your observations in the data chart.
Step 4: Use the reaction chart at the top of this page to determine the blood type.
Step 5: Repeat steps 1-4, using sample 2 and the last three cups of serum.Slide11
Results (worth 8 points)
Place your blood typing results into the chart below
.
Note: Each correct answer in the chart will be worth 1 point. For the chart, simply put “yes” if there is a reaction, or “no” if there isn’t an interaction
Reactions
w/Anti-A
Serum Reactions
w/Anti-B Serum
Reactions
w/ Anti-Rh Serum
Blood Type
Sample 1
Sample 2
Slide12
Analysis and Conclusion (worth 20 points)
Each question is worth 5 points.
To receive full credit for each question, please explain how you came up with your answer (based on the lab you just completed).Slide13
1. What
steps did the body take to stop the bleeding of Chris’s minor scraps before he arrived at the ER? (Refer to the section on hemostasis in your lesson.)Slide14
2. What
blood type did your results show for Chris’s blood type? How did you know this was his blood type? Slide15
3. What
blood types can Chris receive in a blood transfusion? (Refer to the section on blood transfusions in your lesson.)Slide16
4. What
will happen to Chris’s blood if he receives the wrong type of blood transfusion? (Refer to your blood types video notes.)Slide17
You do not need to complete Part 2.