PPT-Diagnostic tests for antibody or antigen

ELISA Immunofluorescence Flow cytometry Western blotting ELISA We use an antibody thats bound covalently to an enzyme to bind antigen direct ELISA antibody that is bound to antigen indirect ELISA. . Using the characteristic of high affinity and specificity of antibody, we can detect or quantitative the antigen. Keep in mind that the antibody is protein, can also be recognized as an antigen. The major principle to determine the antigen-antibody interaction is to separate the bound form of antigen-antibody complex from the free form of either antigen or antibody. . Group . 3. :. M. . Yusron. . Hasani. . (. 105090100111037. ). A. Muammar . Kadafi. . (115090101111012. ). Nira. . Meirit. a. W.. . . (. 115090100111017. ). Putri. . Indisari. . (. 1150901. BCH 462 [practical] . Lab#7. Objective:. -To get familiar with radial immunodiffusion.. -To determine the concentration of unknown antigen.. . -Precipitation . reactions are based on the interaction of antibodies and antigens. They are based on two soluble reactants that come together to make one insoluble product, the precipitate (. Antibodies. Cells of the vertebrate acquired immune system produce antibodies with an exquisite specificity for molecules. CIL:10233 Hippocampal neurons. Actin (red); tubulin (green). western blot of bacterial cell extracts . adio. i. mmuno. a. ssay. . Remember. . Immunoassay reactions may be competitive or non-competitive. Competitive. . . labeled known and patient unknown are added to reaction and “compete” for the target.. . BCH462 [practical]. Objective:. - To learn the technique of . immunoelectrophoresis. .. -Precipitation . reactions are based on the interaction of antibodies and antigens. They are based on two soluble reactants that come together to make one insoluble product, the precipitate (. Agglutination. The interaction between an antibody and a particulate antigen results in visible clumping called agglutination. Particulate antigen include: . Bacteria, . W. hite blood cells, . R. ed blood cells, . Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a mean of generating a measurable result. Immuno. refers to an immune response that causes the body to generate antibodies, . Immunoassay. An immunoassay is a test that uses . antibody. and . antigen. complexes as a . mean . of generating a measurable . result. Immuno. . refers to an immune response that causes the body to generate antibodies, . how to avoid them. What is the problem?. We have a reproducibility crisis in science. The consequence is a enormous waste in materials, time and money, costing an estimated . US$350 million. annually in the US alone!. Dr. Suzan Y.. Antigen. :. It s a substance that may be specifically bound by an antibody (. Ab. ) molecule or T cell receptors (TCR). When this binding lead to the activation of B cells or T cells ,this substance is known as . Dr. . Amany. . Ballow. . Consultant Immunology . Assistant professor KKUH. General Lab Director MDLAB. PhD, Diploma BSHI,MSC.. Objectives . To describe B-cells as the mediators of humoral immunity, (antibody-mediated immunity). Detection By. Precipitation Methods. Dr.T.V.Rao MD. 11/11/2014. Dr.T.V.Rao MD. 1. Beginning of Serology. Serology as a science began in 1901. Austrian American immunologist . Karl Landsteiner. (1868-1943) identified groups of red blood cells as A, B, and O. From that discovery came the recognition that cells of all types, including blood cells, cells of the body, and microorganisms carry proteins and other molecules on their surface that are recognized by cells of the . Research Details . A vaccine-derived human antibody fragment (Fab 1A12) was crystallized alone and in complex with its target antigen, factor H binding protein (fHbp).. In a previous study, Fab 1A12 cross-reacted with fHbp variants that have different amino-acid sequences (antibodies are usually specific to one fHbp variant group)..