PDF-Proc.Natl.Acad.Sci.USAVol.90,pp.11478-11482,December1993ImmunologyDNAv

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ProcNatlAcadSciUSA90199311479knownconcentrationDNAwasstoredin1mMTrispH7801mMEDTAForinjectionsDNAwasdilutedinsaline09NaClVaccineTrialsVaccinetrialsinmicewereaccomplishedbytheadminist

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Proc.Natl.Acad.Sci.USAVol.90,pp.11478-11482,December1993ImmunologyDNAv: Transcript

ProcNatlAcadSciUSA90199311479knownconcentrationDNAwasstoredin1mMTrispH7801mMEDTAForinjectionsDNAwasdilutedinsaline09NaClVaccineTrialsVaccinetrialsinmicewereaccomplishedbytheadminist. Proc.Natl.Acad.Sci.USA90(1993)10391days.Whenthecellswereforamicroinjectionexperiment,coverslipswerefirstmarkedwithadiamond-tippentofacilitatethelocalizationandvisualizationofmicroinjectedcells.After8- Proc.Natl.Acad.Sci.USA90(1993)424Age(months)200.0120z0.010laaCD-_-0.008oo100CQ0.006coCD~0o0.004x0NuclearDNATI0.002F0.04242a100000Q0la)co0x0Age(months)FIG.1.Steady-stateoxidativeDNAdamageincreaseswitha Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)inasignificantlyfasterreaction.Theformationrateofnativemoleculesonthisfastrefoldingchannelcorrespondstotherateexpectedforfoldingtooccurintheabsenceofpopulatedintermediate Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA88(1991)5593Table1.YeaststrainsNuclearMitochondrialStraingenotypegenotypeRef.947aade2ura3-52p09ID41-6aadellysi15161p+15C1036mit-;COXJintron116splicing-defectivepointmutant(trans- Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest

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