Total Precision Total PrecisionAUTOLYTE Cotinine EIA using 500 ngmL Cotinine as cutoff calibrator All the samples were AUTOLYTE GCMS con31rmed the result obtained by the DRI Cotinine Assa ID: 941508
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Qualitative (mA/min) Total Precision Total PrecisionAUTO-LYTE Cotinine EIA using 500 ng/mL Cotinine as cutoff calibrator. All the samples were AUTO-LYTE* GC/MS con rmed the result obtained by the DRI Cotinine Assay.** Two out of the three discrepant samples were borderline positive. AUTO-LYTE* GC/MS con rmed the result obtained by the DRI Cotinine Assay.** Two out of the four discrepant samples were borderline positive.Sensitivity, de ned as the lowest concentration that can be differentiated from the negative Compounds structurally related to cotinine were tested for cross-reactivity. Results are CompoundConcentration ( g/mL)Result3-Hydroxy-Cotinine12.5PositiveNiacinamide500NegativeNicotine500NegativeNicotinic Acid500NegativeNicotinic Acid N-Oxide500NegativeCompounds structurally unrelated to cotinine produced negative results at the concentrations listed below: 11-Nor-Valproic AcidCotinine Assay.
No interference was observed in urine samples containing compounds at the concentrations listed below. Urine pH was also studied for possible interference. CompoundConcentrationAcetaminophen100 g/mLAcetone1000 mg/dLAscorbic Acid1250 mg/dLAspirin100 g/mLCaffeine100 g/mLCreatinine500 mg/dLEthanol1 g/dLGalactose10 mg/dLGamma Globulin500 mg/dLGlucose3000 mg/dLHemoglobin300 mg/dLHuman serum Albumin500 mg/dLIbuprofen100 g/mLOxalic Acid100 mg/dLRibo avin7.5 mg/dLSodium Chrloride0.5 g/dLUrea0.8 g/dLpH range4-9 Tel: +49 (0) 851 886 89 0 Baselt RC: Disposition of toxic drugs and chemicals in Man, ed 3. Chicago, IL, Year Book tobacco users. Clin. Pharmacol. Ther., 30, 201 (1981).Langone JJ, Gjika HB and H Van Vunakis. Nicotine and its metabolites. Radioimmunoassays for nicotine and cotinine. Biochemistry, 12, 5025 (1973).Jarvis MJ, Tunstall-Pedoe H, Feyerabend C and C Vesey. Comparison of tests used to Pojer R,
Whit eld JB, Poulos V, Eckhard IF, Richmond R and WJ Hensley. Matsukura, S., etal., Effects of Environmental Tobacco Smoke on Urinary Cotinine Haley NJ, Axelrad CM and KA Tilton. Validation of self-reported smoking behavior: Biochemical analyses of cotinine and thiocyanate. Am. J. Public Health, 73, 1204 (1983).www.thermoscienti c.com/diagnostics Technical Support: 2 3 DRI® Cotinine Assay For In Vitro Diagnostic Use Precautions and WarningsThis test is for in vitro diagnostic use only. The reagents are harmful if swallowed.0.09% sodium azide. Avoid contact with skin and mucous membranes. Refer to SDS for additional precautions, handling instructions, and 0.2% bovine serum albumin (BSA). Avoid contact with skin and mucous membranes. Avoid inhalation. May cause skin or inhaled allergic reaction. In the case of accidental spill, clean and dispose of material according to your laboratory’s SOP, BS