Gentamicin Induces Renal Morphopathology in Wistar Ratsa - PDF document

Gentamicin Induces Renal Morphopathology in Wistar Ratsa Renal en Ratas WistarVanessa Barboza de Souza; vio Freitas de Lucena; 60 MATERIAL AND METHODMale Wistar rats 8-12 weeks, weighing 200-250g,which a 12:12h light: dark cycle was maintained. Twenty-treatment intraperitoneally. The control group was treatedrespectively. After treatment, the animals in all groups werefor histopathologic examination, by light microscopy.Histochemistry. The renal tissues slides were stained withperiodic acid Schiff (PAS) reagent and examined lightmicroscopy. The PAS reaction stains carbohydrates andthe basal membrane that underlies epithelia. Three codedslides from each kidney were examined in a blinded manner.Immunohistochemistry. With some modifications, theused technique followed the recommendations of Taylorantibody LSAB (complex DAKO). The slide wereThe morphology of the PAS stained structures of the renalmembrane of renal glomerule and parietal cells. Themicroscope (with camera) connected to a microcomputer. The renal morphopathological and thestatistically with the Kruskal-Wallis Test and Dunn'sMultiple Comparisons Test (5% statistical significance).RESULTSfound through histochemical method PAS to the diferenceshistological features, illustrated in Figs. 2A and 2D. However,illustred in Figs. 2B, 2C and 2E, respectively. There was agentamicin groups (P�AS). Group 01 vs. Control group (p0.05). Group 02 vs.Control group (p)()roup 04 vs.�.00;
. G;&#xroup;&#x 01 ;&#xvs. ;&#xGrou;&#xp 02;&#x p00;Control group (p)()01 vs. Group03 (p)()up 03�.00;
. G;&#xroup;&#x 02 ;&#xvs. ;&#xGro-;�.80;(p0.05). Group 02 vs. Group 04 (p)�.00;
. G;&#xroup;&#x 03 ;&#xvs. ;&#xGrou;&#xp 00;&#x.300;4 (p0.05).DE SOUZA, V. B.; OLIVEIRA, R. F.; LUCENA, H. F.; FERREIRA, A. A. A.; GUERRA, G. C. B.; FREITAS, M. L.; QUEIROZ, K. C. S. & DE NIOR, R. F.renal morphopathology in Wistar rats. 61 of kidney samples. A. Glomeruli andPeriodic acid Schiff (PAS) stain. B.gentamicin. PAS. C. Tubular marked) is observed in group 4. PAS. D.protein marker. E. Appearance inprotein marker.Table I. Scores of renal histological alterations in Wistar ratDE SOUZA, V. B.; OLIVEIRA, R. F.; LUCENA, H. F.; FERREIRA, A. A. A.; GUERRA, G. C. B.; FREITAS, M. L.; QUEIROZ, K. C. S. & DE NIOR, R. F.renal morphopathology in Wistar rats. ScoresHistologicalcharacteristics1 (unaltered)2 (slight)3 (moderate)4 (severe)AAlterations of parietalcells. Bowmancapsule.Unalteredcharacteristics.Cells parietalalterationsmorphological.Decreased Bowmancapsule.Cells alteredmorphology. decreasedBowmans Capsule.BBasal membrane(PAS).Unalteredcharacteristics.Discret interruptionbasal membrane.Interruption basalmembrane.Intense interruptionbasal membrane.CMesangial cells.cellular apoptosis(PAS& P53)Unalteredcharacteristics.Discret increased ofmesangial cells.Moderate increased ofmesangial cells.Apoptosis.Increased mesangialcells. Apoptosis.DTubule proximal cells;cellular apoptosis.(PAS & P53)Unalteredcharacteristics.Integral brush-bordermembrane; leveldesquamationcells.Interruption brush-bordermembrane. Apoptosisand tubular necrosis.Complete interruptionbasal membrane.Apoptosis/ necrosis. adequate fluid volume control. Aminoglysosides-inducedgentamicin has been shown to induce proximal tubular injury,In different studies it was was shown that gentamicin, 1997). In the present study,gentamicin treatment (p)Another in vitro studyshowed the contractive and proliferative effects of gentamicinand tissue homeostasis of most multicellular organisms., 2006). Further, among the varioussubcellular organelles potentially involved in apoptosis, bothDE SOUZA, V. B.; OLIVEIRA, R. F.; LUCENA, H. F.; FERREIRA, A. A. A.; GUERRA, G. C. B.; FREITAS, M. L.; QUEIROZ,K. C. S. & DE ARANIOR, R. F. a renal en ratas Wistar. n mayor, el riticos aminoglicosidos en la corteza renal tiene como consecuencia efectos endico de Schiff (PAS) y tincicomo un marcador para la apoptosis celular, permitiendo la deterenales fueron apoptosis celular, interrupciPALABRAS CLAVE: Nefrotoxicidad; Aminoglycosides; Expresin de la protena P53; Alteraciones morfopatolrenales. Rev. AMRIGS, 50(2)Chambers, H. F. Gibey, R.; Dupond, J. L.; Alber, D.; Leconte des Floris, R.& Henry, J. C. Predictive value of urinary N-acetyl-beta-. Clin. Chim. Acta.,116(1)Han, S. Y.; Chang, E. J.; Choi, H. J.; Kwak, C. S.; Park, S.B.; Kim, H. C. & Mun, K. C. Apoptosis by cyclosporineTransplant. Proc., 38(7) DE SOUZA, V. B.; OLIVEIRA, R. F.; LUCENA, H. F.; FERREIRA, A. A. A.; GUERRA, G. C. B.; FREITAS, M. L.; QUEIROZ, K. C. S. & DE NIOR, R. F.renal morphopathology in Wistar rats. Iwakura, R.; Martins, A. C. P.; Tucci Junior, S.; Pastorello,M. T.; Suaid, H. J.; Cologna, A. J. & Carneiro, A. D. C.Acta Cir. Bras.,Jian, M.; Wei, Q.; Pabla, N.; Dong, G.; Wang, C. Y.; Yang,T.; Smith, S. B. & Dong, Z. Effects of hydroxyl radicalcell apoptosis and nephrotoxicity. nez-Salgado, C.; Eleno, N.; Morales, A.; PBarriocanal, F.; Arpez, F. J. & Novoa- Toxicol. Appl. Pharmacol., 223(1)guez-Barbero, A.; RodrInvolvement of phospholipase A2 in gentamicin-inducedPolat, A.; Parlakpinar, H.; Tasdemir, S.; Colak, C.; Vardi,N.; Ucar, M.; Emre, M. H. & Acet, A. Protective role ofRodriguez-Barbero, A.; Lpez-Novoa, J. M. & ArExp. Nephrol., 5(1)Rodriguez-Barbero, A.; Rodriguez-Lopez, A. M.; Gonzalez-rat mesangial cells. A role for PAF. Servais, H.; Van Der Smissen, P.; Thirion, G.; Van der Essen,G.; Van Bambeke, F.; Tulkens, P. M. & Mingeot-Leclercq, M. P. Gentamicin-induced apoptosis in LLC-Toxicol. Appl. Pharmacol., 206(3)Souza, V. B.; Oliveira, R. F. L.; Ferreira, A. A. A. & Aranior, R. F. AlteraArqTaylor, C. R.; Shi, S. R.; Chiwun, B.; Young, L.; Imam, A.androgen receptor, estrogen receptor, progesterone re-ceptor, P53 protein, proliferating cell nuclear antigen,(\n(\n(\n!(\t\t\b\f\n3-2332*..-DE SOUZA, V. B.; OLIVEIRA, R. F.; LUCENA, H. F.; FERREIRA, A. A. A.; GUERRA, G. C. B.; FREITAS, M. L.; QUEIROZ, K. C. S. & DE NIOR, R. F.renal morphopathology in Wistar rats. 64