Jessica Baker Supervisor Dr Deanna Gibson UBC MURC 2011 Inflammatory Bowl Disease IBD Colitis IBD Ulcerative Colitis and Crohns Disease It is a chronic relapsing immunologicallymediated disorder in the gastrointestinal tract ID: 150465
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Slide1
Lavender Protects Against Infectious Colitis
Jessica Baker
Supervisor: Dr. Deanna Gibson
UBC MURC 2011Slide2
Inflammatory Bowl Disease (IBD): Colitis
IBD: Ulcerative Colitis and
Crohn’s
Disease
It is a chronic, relapsing, immunologically-mediated disorder in the gastrointestinal tractUnknown cause, although onset and activation is thought to develop in predisposed individualsCurrently there are limited therapeutic options Slide3
Bacteria-Induced Colitis Model
Use a bacterium called
Citrobacter rodentium:
a mouse-specific intestinal pathogen
Some important inflammatory factors: TNF-α, IFN-γ, iNOS, IL-22, MIP-2α and IL-10Results in intestinal damage similar to human colitis Slide4
Lavandula x intermedia
: Okanagan Lavender
Developed in Dr. Soheil Mahmoud’s Lab at UBC Okanagan
Objective: to improve oil composition and yield
Terpene
Wild
Type Oil
Okanagan Lavender Oil
Cineole (%)
13.73 ± 1.28
27.09 ± 1.78
Camphor (%)
11.16 ± 0.35
3.03 ± 0.26 Linalool (%)35.92 ± 2.2736.28 ± 4.16 Linalool acetate (%)25.34 ± 2.341.86 ± 0.36 Borneol (%)3.55 ± 0.59 21.01 ± 3.63
Desautels
, A, et al. “Suppression of Linalool Acetate production in
Lavandula x
intermedia
.”
Natural
Products Communications
4.11 (2009): 1533-1536.
Santos, F.A., et al. “1,8-cineole (eucalyptol), a
monoterpene
oxide attenuates the colonic damage in rats on acute TNBS-colitis.”
Food and Chemical
Toxicology
42 (2004): 579-584.
Tung, Yu-Tang, et al. “Anti-inflammation activities of essential oil and its constituents from indigenous cinnamon (
Cinnamomum
osmophloeum
) twigs.”
Bioresource
Technology
99 (2008): 3908-3913.Slide5
Hypothesis
We hypothesize that Okanagan Lavender
oil
will decrease the severity of infectious colitis due to its ability to increase pathogen clearance as well as its ability to decrease inflammation and associated inflammatory mediatorsSlide6
Group 1: Mineral oil
OR
Group 2: Lavender oil
Orally gavaged throughout 10-day infection
B6
(C57BL/6)
Mice
OR
C3H
(C3H/HeOuJ)
Mice
Orally gavaged with
C. rodentium
for 10 days
Monitored morbidity
and mortality
Procedure:Slide7
Lower Gastrointestinal Tract
Cecum
Distal Colon
RNAlater: extract the RNA
for q-PCR
H&E stain: Scoring
Mesenteric Lymph Nodes
AND
Spleen
Bacterial counts to
assess systemic infectionSlide8
Lavender-treated mice susceptible to lethal infectious colitis (C3H mice) showed a drastic decrease in mortality
Lavender-treated mice that survive colitis (B6 mice) showed significantly less morbidity
(
P< 0.05; Mann-Whitney T-test)
B6 Mice
C3H MiceSlide9
micrographs of the cecum
Lavender Oil significantly reduces
Edema
Infiltration
Epithelial hyperplasia
Goblet cell depletion
Epithelial damage
(
P< 0.01, Mann-Whitney T-test)
Uninfected
Infected + Mineral Oil
Infected + Lavender OilSlide10
In
Vitro
and In
Vivo
Evidence That Lavender Oil Can Directly Kill This Pathogen
Mineral Oil
Lavender Oil
Lavender Mineral
Oil Oil
Colony Forming Units
MLN
Spleen
In
Vivo
In
VitroSlide11
q-PCR Results
The mice orally
gavaged
with lavender oil showed a significant modulation in the immune response
(P< 0.05, Mann-Whitney T-test) Slide12
Immunofluorescence Results
Infected +
Infected +
Lavender-treated mice showed a drastic decrease in immune cell infiltrationSlide13
Conclusion
The significant decrease in morbidity, cecal tissue damage, pro-inflammatory cytokine production and immune cell infiltration provide strong support for our hypothesis that Okanagan Lavender Oil protects against infectious colitis
Two mechanisms
Antimicrobial
Immune Modulation
Terpene
Okanagan Lavender Oil
Cineole (%)
27.09 ± 1.78
Camphor (%)
3.03 ± 0.26
Linalool (%)
36.28 ± 4.16
Linalool acetate (%)1.86 ± 0.36Borneol (%) 21.01 ± 3.63Slide14
Future
Results so far warrant further analysis
Isolate and test Lavender components
Need to do more animal studies with higher N number and varied dose amounts
Need to examine overall health in other areas of the body Potential outcomes:An anti-inflammatory drug for chronic intestinal diseases such as colitis An antimicrobial drug for intestinal infectious diseasesSlide15
Acknowledgments
Dr. Deanna Gibson (Supervisor)
Dr. Soheil Mahmoud and Lab
Dr.
Sanjoy GhoshSamantha MakarenkoKirsty BrownDr. Mark Rheault Brie Matier
Irving K. Barber Endowment fund
Everyone with MURC 2011