Figure 3Reducing power of aqueous and ethanolic extracts of Figure 5 Superoxide radicals scavenging activity of watercressNasturtium officinale ID: 126407
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was added to3.0 mL of watercress extracts or standard antioxi-dant solution (100 µg/mL). The mixture was mixedand after 10 min the absorbance was measured at517 nm. The DPPHïconcentration (mM) in the reac-tion medium was calculated from the calibrationcurve (R2: 0.9998).Absorbance = 6.5781 [DPPHï mM] + 0.0058This activity was calculated by the equation:DPPHïscavenging effect (%) = [(A0-A1)/A0 100]where A0was the absorbance of the control and A1was the absorbance of watercress or BHT (19).Superoxide anion scavenging activity Figure 3.Reducing power of aqueous and ethanolic extracts of Figure 5. Superoxide radicals scavenging activity of watercress(Nasturtium officinaleµg/mL of extracts, BHA, BHT and -tocopherol were assayed at 517 nm. Figure 4.DPPH free radical scavenging activity of watercress Figure 6. Changes in rat liver lipid peroxidation at 532 nm. *p 0.05, **p #not significant, when compared with the control g/mLBSARESULTS AND DISCUSSIONradicalscavenging is thought to be due to their hydrogendonating ability. DPPHis a stable free radical andaccepts an electron or hydrogen radical to become astable diamagnetic molecule. The model of scav-enging the stable DPPHis a widely used method toevaluate antioxidant activities compared to othermethods (32). Table 1 shows the scavenging effectof watercress extracts on the DPPHradical, wherethe scavenging effect, as the absorbance differencefrom the control, was calculated. The radical scav-enging activity using a DPPHgenerated radical,was tested with water and ethanol extracts of water-cress, and standards. The change in absorbance ofDPPHradical caused by antioxidants is due to thereaction between the antioxidant molecules and theradical, which results in the scavenging of the radi-cal by hydrogen donation. It is visually noticeable asa discoloration from purple to yellow (16). All ofamounts of watercress extracts exhibited significant-ly higher activities than the control (p )scavenging effect of water and ethanol extracts ofwatercress and standards on the DPPHradical&#x 0.0;. T;&#xheTj;&#x -15;&#x.794; -1;&#x.248; TD;&#x 0.1;Ѓ ;&#xTw 0;decreased in order of ethanol extract water extract&#x 0.0;. T;&#xheTj;&#x -15;&#x.794; -1;&#x.248; TD;&#x 0.1;Ѓ ;&#xTw 0; BHA which were 87.08, 75.15, and 61.08% at the