Bacteria Hopkins Microbiology Course Presented by Ying Lei 22 July 2010 Reduction Process Fe 3 only ferrihydrite FeOOH 04H 2 O orangered solid Fe 3 Fe 2 magnetite Fe ID: 139661
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Slide1
Iron-Reducing Bacteria
Hopkins Microbiology Course
Presented
by Ying
Lei
22 July 2010Slide2
Reduction Process
Fe
3+
only
– ferrihydrite (FeOOH · 0.4H
2
O)
– orange/red solid
Fe
3+
/Fe
2+
– magnetite (Fe
3
O
4
)
– black, ferromagnetic solid
Fe
2+
only
– ferrous iron
– black, non-magnetic solidSlide3
Redox Tower
Reduced Species
Oxidized Species
Redox Potential (E
0
’)[CH2O]CO2-470 mVFe2+αFe2O3-280 mVFe2+αFeOOH-280 mVCH4CO2-250 mVH2SSO42--220 mVFe2+γFeOOH-100 mVFe2+Fe(OH)30 mVFe2+Fe3++770 mVH2OO2+820 mV
CH3CH(OH)COO- + 12 FeOOH + 4 H2O 3 HCO3- + 12 Fe2+ + 22 OH-
lactateC0
iron (III)Fe3+
CO2C4+
iron (II)Fe2+
Canfield
et al
,
Aquatic Geomicrobiology
(2005)Slide4
Enrichment Medium
Component
Amount
Purpose
basal
marine mineral medium1 Lbasic saltssodium lactate4.5 g (30 mM)carbon sourceNaHCO330 mL (30 mM)pH bufferiron (III) hydroxide30 mL (40 mM)ferric iron source7-vitamin solution30 mLcofactorsSL 101 mLtrace elementsSe-Wo solution1 mLtrace elementsAdjust to pH 7.2 in Pfennig bottles and incubate under anoxic conditions.Hopkins Microbiology Course (2010)Slide5
Sample Collection
Canfield
et al
,
Aquatic Geomicrobiology
(2005)Slide6
Sample CollectionSlide7
Successful Enrichments
MarcusSlide8
Thank You
All Instructors and Students
Chris
Marcus
BlancaSlide9
Additional SlidesSlide10
Iron Solubility
OzCoasts (www.ozcoasts.org.au)