PPT-TISSUE PROCESSING Fixation

Dehydration Clearing Impregnation Embedding and blocking Section cutting Routine staining COMMONLY USED FIXATIVES Formalin MC routine Glutaraldehyde. Prepared by : Reem Aldossari. THE GENERAL VISCRA OF THE RAT. Definition of Fixation . In the fields of . histology. , . pathology. , and . cell biology. , . fixation. is a . chemical process. by which . TOPIC:. FIXATION. SUBMITTED BY. RINSEENA M. GAFOOR. SUBMITTED TO. Mr. REJO JACOB JOSEPH. &. Mrs.ARCHANA MADAM. FIXATION. It is the process by which the morphology and . microscopic anatomy of the tissue is preserve as. Bogers, . Ga. ë. lle. . Boulet. AMBIOR . Group, University . of . Antwerp. AML-. Riatol. , Antwerp. Oncology imaging & personalized medicine, IOS. Brussels, 29 October 2011. Tissue quality for molecular biology and pathology. Tissue Processing. Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. Techniques. By. DR ANYANWU GE. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . Light and Electron Microscopes. Tissue Preparation. E-mail: . a.al-nuaimi@sheffield.ac.uk. E. mail: abdulameerh@yahoo.com . Prof. . Abdulameer. Al-. Nuaimi. Histology :. Is a . branch of anatomy that deals with the minute structure of animal and plant . THE GENERAL VISCRA OF THE RAT. Definition of Fixation . In the fields of . histology. , . pathology. , and . cell biology. , . fixation. is a . chemical process. by which . biological tissues. are . Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. BeomKang. Huh. Sarah Czaplewski. Client: Dr. Wan . Ju. Li. Advisor: Professor Block. Cartilage bioreactor. Overview. Joint Trauma and Degeneration. Current Treatments. Future Directions. Non-Destructive Testing for Tissue Engineering . It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. . . . . 10. % Neutral Buffered Formalin is the routine fixative. . Tissue Processing. Tissue processing consists of 3 steps. :. . 1-. Dehydration . . 2-. Clearing. . . These techniques included:. *Anesthetization. *Fixation. *Embedding. *cutting. *mounting onto slides. *Staining. *Washing. *mounting . *preservation. Animal experiments are necessary for a better understanding of diseases and for developing new therapeutic strategies. Animal experiment include Mouse, Rat, Rabbit, Hamster,..... Embedding. Sectioning. Staining. Fixation. It is important to . maintain. cells in as life-like a state as possible . to prevent post-mortem changes as a result of putrefaction (destruction of tissue by bacteria or fungi) and autolysis (destruction of tissue by its own enzymes). . Purpose Statement. At the end of the lecture student should be able to . describe. the . Composition of tissues. Histochemical. techniques. Learning Objectives . At the end of the lecture the student should be able to.