Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188Histopathologic altera - PDF document

Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188Histopathologic alterations observed in sh gillsas a tool in environmental monitoringFlores-Lopes, F.* and Thomaz, AT.Cyanocharax alburnusCyanocharax alburnuscollected seasonally and processed using routine histologic techniques for xing and embedding in parafn and staining Keywords:Cyanocharax alburnusAlterações histopatológicas observadas nas brânquias de peixesCyanocharax alburnusCyanocharax alburnuse coradas com H&E. As principais alterações observadas em ambas as espécies foram alteração da estrutura do epitélio, Palavras-chave:Cyanocharax alburnus Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188effects observed, and the utilisation of natural environments of the species Astyanax fasciatus (Cuvier, 1819) and Cyanocharax alburnus The samplings were carried out using a seine net 5 – Barra do Ribeiro, municipality of Barra do Ribeiro two were in Rio Caí (7 – next to the bridge with highways both in the municipality of Gravatai) (Figure 1). The samplings were carried out seasonally over a period of to December 2003 in the tributary rivers, and the second one week. In the laboratory, the material collected was Twelve specimens, when possible, of the species Cyanocharax alburnus1. Introductioneffects of pollutants in sh is to examine their organs for and have a large surface area in contact with the external Histopathologic studies have been used to evaluate organisation (Stebbing, 1985). Currently, physiologic and Various authors such as Karlsson-Norrgren et al. al. (1997), Stentiford Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188alteration (histopathologic alterations index - HAI) which in the organ; and 50 and 100 indicate severe lesions; The degree of signicance was 95%. Statistical analyses of dehydration in ethanol and then impregnation and hematoxylin and eosin (H&E) for general visualisation effects in tissues associated with the functioning of the Jacuí RiverCaí RiverGravataí RiverSinos River Figure 1. Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188vacuolisation and hyperplasia of the epithelium of the primary lamella. In the secondary lamella, there were hypertrophy structure, pyknotic nucleus of the pillar cells, secretion A. Fasciatus,sampling sites Sinos, Gravatai RS118 and Gravatai PC Cyanocharax alburnusDepartment of Water and Waste. The data for the Rio dos Gravatai were provided by CORSAN – Rio Grande do Cyanocharax alburnus were analysed, including 65 females Cyanocharax alburnusTable 1. Cyanocharax alburnus StageHistopathologic alterations in the gillsIHypertrophy and hyperplasia of gill epitheliumIIHemorrhage and rupture of lamellar epitheliumIIILamellar aneurysmNecrosis and cell degeneration Lamellar telangiectasis Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188of aneurysms of various sizes in some secondary lamellae Figure 2. a b c d a b Figure 3. Cyanocharax alburnus Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188(2 and 3) were observed in Praia das Pombas and in lago C. alburnusyanocharax alburnusvaried from 1 to 114 and from 1 to 115, respectively, with C. alburnussignicant (p 0.005). Tukey’s test demonstrated that the C. alburnusresults demonstrate that based on this method this site can alburnus a b c d Figure 4. Histologic sections of gills of specimens of Cyanocharax alburnus. a) 1 – alteration of normal structure of primary lamella; 2 – secretion of mucus; 400X; b) 3 – cyst with parasitic protozoans; 4 – concentration epithelial cells; 5 – epithelial Figure 5. Cyanocharax alburnus Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188of its high mean level of fecal coliforms and low means Cyanocharax alburnusalteration of structure, pyknotic nucleus of the pillar cells, According to Klontz (1972), sh are intimately associated physiologic measurements in the sh. In general, reacions alburnusThe principal components analysis produced two putting them in class IV for fecal coliforms and class I for Table 2. Cyanocharax alburnus Cyanocharax alburnusTot.Tot. Braz. J. Biol., 2011, vol. 71, no. 1, p. 179-188other stressors that were not analysed affected the gills lamellae, where there was a large quantity of vessels high loads of domestic and industrial wastes which are Cyanocharax alburnusclass I. These results indicate that probably other factors the importance of the addition of histopathologic analysis in and degradations of anthropic origin that can compromise the quality of the ecosystems, all together serving as tools hyperplasia, lamellar fusion, excessive production of and formation of aneurysms. Inammation, hyperplasia, C. alburnusgills of these individuals had been affected by the action of various stressors. As a consequence of the epithelial by compensating for the low entrance of oxygen (Fernandes cells, hyperplasia of the chloride cells and proliferation of Astyanax altiparanaeProchilodus lineatusstream in the city of Londrina, Brazil differed a little from Braz. J. 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