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A. cinnamomeaYen,Yang,2002),andantibioticproperties(ChenandYang,1995). A. cinnamomeaYen,Yang,2002),andantibioticproperties(ChenandYang,1995).

A. cinnamomeaYen,Yang,2002),andantibioticproperties(ChenandYang,1995). - PDF document

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A. cinnamomeaYen,Yang,2002),andantibioticproperties(ChenandYang,1995). - PPT Presentation

A cinnamomeahasfarexceededthesupplyanditisnowconsideredamongthemostexpensiveherbalmedicinesonthemarketmorethanThehighdemandis A off C kanehirae trunkandendangeringthetreespecieswhichisuniquetoT ID: 93029

cinnamomeahasfarexceededthesupply anditisnowconsideredamongthemostexpensiveherbalmedicinesonthemarket(morethanThehighdemandis A. off C. kanehirae trunkandendangeringthetreespecies whichisuniquetoT

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A. cinnamomeaYen,Yang,2002),andantibioticproperties(ChenandYang,1995).Therefore,demandfor A. cinnamomeahasfarexceededthesupply,anditisnowconsideredamongthemostexpensiveherbalmedicinesonthemarket(morethanThehighdemandis A. off C. kanehirae trunkandendangeringthetreespecies,whichisuniquetoTaiwan.Inanefforttoresolvetheconservationissue ZLWKRXWVDFUL¿FLQJWKHPHGLFLQDOEHQH¿WVVFLHQWLVWVIURPacademiaandthepharmaceuticalindustryhavebeenintensivelyworkingtodevelop A. cinnamomeaproductsinthelaboratory.majorapproachthelaboratorystudy A. cinnamomea fruitingbodyonthe C. kanehiraecommerciallyavailablenutrientmedia.NoscientificreportonthismethodhadbeenpublisheduntilveryrecentlywhenChangandWang(2005)reportedan in vitro fruitingbodyformationofan A. cinnamomea isolateonefforts A. cinnamomea in vitro throughnovelwoundingprocedure.Thereportshouldstimulatenewthinkingonthestudyof A. cinnamomea fruiting in vitro MICROBIOLOGY In vitro induction of fruiting body in Antrodia cinnamomea – a medicinally important fungusJyh-Yuan Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Shou-Feng, Hualien 97401, TAIWAN in vitrogrownfruitingbodiesanalyzedbyHPLCshoweddistinct Keywords: Abioticstress; Antrodia cinnamomeaWound LIN et al. — Antrodia cinnamomea fruiting body induction 271 productprofilesatdifferentstagesof A. cinnamomea Wecontainhigherproportionofpolarcompoundsthanthefruitingbodytissues,whichcontainedmorenon-polarcompounds.Thisisimportantinformationforthepharmaceuticalcompaniesinterestedinthemedicinalapplicationof A. cinnamomeaThepresentstudymay C. kanehiraeoff A. cinnamomeanecessary. Acknowledgements. ThisworkwassupportedbyDevelopment(CEPD-K-1010toTZW)andbyanROC(NSC-92-2311-B-259-002 LITERATURE CITEDBulter,G.M.andA.E.Wood.1988.Effectsofenvironmentalfactorsonbasidiomedevelopmentintheresupinate Phellinus contiguousTransactions 90: T.-T.W.-N. Antrodia cinnamomumnov. Cinnamomum kanehiraiinTaiwan.Mycol.Res. 99: Chang,T.-T.andW.-N.Chou.2004. Antrodia cinnamomea reconsideredand A. salmoneasp.nov.on Cunninghamia Taiwan. T.-T.W.-R.Wang. Antrodia cinnamomea RQDUWL¿FLDODJDUPHGLD%RW%XOO Chen,C.-H.andS.-W.Yang.1995.Newsteroidacidsfrom Antrodia cinnamomeafungalparasiteof Cinnamomum micranthum. D.-P.Wu,Triterpenoids Antrodia cinnamomea Hideo,H.,T.Naoki,andM.Sawao.1985.Isolationand LGHQWL¿FDWLRQRIF\FORRFWDVXOIXUDIUXLWLQJERG\LQGXFLQJsubstance,producedby Streptomyces albulusTO447.Agric. Hseu,Y.-C.,W.-C.Chang,Y.-T.Hseu,C.-Y.Lee,Y.-J.Yech,P.-C.Chen,J.-Y.Chen,andH.-L.Yang.2002.Protectionofoxidativedamagebyaqueousextractfrom Antrodia camphoratamyceliainnormalhumanerythrocytes.Life Lee,I.-H.,R.-L.Huang,C.-T.Chen,H.-C.Chen,W.-C.Hsu, Antrodia camphorataexhibitanti-hepatitisviruseffects.FEMSMicrobiol.Lett. Tarui.fruitingbodyinducingsubstancein Favolus arculariusTA7 Actinomycetes.Agric.Biol.Chem. 48: Figure 4. A. cinnamomea whitemycelia(A),redmycelia(B),fruitingbodygrownon C. kanehirae in vitroMEAplateswithcottonswabrubbingtreatment(D).Severallowtemperature,increasedaeration,increasedhumidity,suitablelightradiation,andwoundingtreatmentsarecommonlyused.Weadaptedsimilartreatmentfor A. cinnamomeaandfoundthreefactors,i.e.additionofaceticacid,airexchange,andwoundtreatments,haduniqueeffectsongrowth.ThewoundingtreatmentwasfoundThisreportopensfurtheravenuesforinductionof A. cinnamomea fruitingbody in vitro A. cinnamomea Themethanolextractionof A. cinnamomeahasbeenusedtostudyitssecondarymetabolitecomposition(WuandChiang,1995;Cherngetal.,1996).Sincethecompositionofsecondarymetabolitesisanimportantfactortoinfluencethemedicinalactivitiesofherbal