Brucella spp. Agent Specific Training PowerPoint Presentation

Brucella  spp. Agent Specific Training PowerPoint Presentation

2018-12-24 4K 4 0 0

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CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp. Brucellosis Worldwide. . . Brucellosis can be found worldwide. More common in countries that do not have effective public health and domestic animal health programs. ID: 745283

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Presentations text content in Brucella spp. Agent Specific Training

Slide1

Brucella

spp.Agent Specific Training

CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp

Slide2

Brucellosis Worldwide

Brucellosis can be found worldwideMore common in countries that do not have effective public health and domestic animal health programsMediterranean Basin (Portugal, Spain, Southern France, Italy, Greece, Turkey)MexicoSouth and Central AmericaAsiaAfricaCaribbeanMiddle East

CDC

Slide3

Brucellosis in the United States

B.

abortus

present in wild elk and bison

B.

suis

– present in feral swine

B.

melitensis

– foreign animal disease

B.

canis

- signs of this disease are different in both genders of dogs

Rita

Traxler

, CDC

CDC Public Health Image Library #4876 http://phil.cdc.gov/phil/home/asp

CDC Public Health Image Library #6327 http://phil.cdc.gov/phil/home/asp

Slide4

Sources of Human Infection in the U.S.

Ingestion of imported, unpasteurized dairy products.Contact with meat and other tissues from infected animalsLaboratory exposures - Aerosol exposure represents a significant risk*infectious dose as low as 10-100 organisms

CDC Public Health Image Library #12344, Dr. Michael Schwartz http://phil.cdc.gov/phil/home/asp

Slide5

Brucella ssp.

Caution:

Brucella

species are highly pathogenic and have caused laboratory acquired infections. It should only be manipulated inside a Biological Safety Cabinet (BSC).

This is the most commonly acquired bacterial infection associated with clinical laboratory work.

Gathany

, James (Photographer). (2005). CDC Public Health Image Library, #8407. http://phil.cdc.gov/phil/home/asp.

Slide6

Brucellosis Warning Signs

Communication between the laboratory and Physician/Epidemiologist is Critical!

Extremely variable

Fever, headache, chills

Body and backache

Intermittent, irregular fever – “Undulant fever”

Chronic fatigue syndrome-like symptoms

Arthritis

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CDC Public Health Image Library #13509 http://phil.cdc.gov/phil/home/asp

Slide7

Brucella

spp.Specimen Sources

Bone marrow or whole bloodJoint or abdominal fluidSpleen, liver abscessesSerum

CDC Public Health Image Library #3438/Dr. Chen http://phil.cdc.gov/phil/home/asp

Slide8

Brucella

spp.General Characteristics

Gram Stain Small, Gram-negative coccobacillusGenerally, faintly stainingSlightly larger than Francisella

tularensis

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Brucella

spp.

gram stain

CDC Public Health Image Library #1901 http://phil.cdc.gov/phil/home/asp

Slide9

Brucella spp.

General Characteristics

Culture 48-72 hours SBA at 35ºC0.5 – 1.0 mm coloniesNon-hemolyticCO2 Enhancement/requirementSBA and CHOC supports growth

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B.

melitensis

mature culture

CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp

Slide10

Diagnostic assays for

Brucella spp.

Diagnostic

assay

Result

Gram stain

Tiny, faintly staining GNCB

Culture growth and morphology on solid medium

Non-pigmented, non-hemolytic, translucent, gray-white, pinpoint

and smooth

Oxidase

Positive

Urea

Strong Positive

Catalase

PositiveX&V factors Not requiredSatellite growthNegative

CDC Public Health Image Library – Image #12288

La’Vonda

Benbow, NCSLPH

La’Vonda

Benbow, NCSLPH

Slide11

Brucella speciation


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