CDC Public Health Image Library 1928 httpphilcdcgovphilhomeasp Brucellosis Worldwide Brucellosis can be found worldwide More common in countries that do not have effective public health and domestic animal health programs ID: 745283
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Slide1
Brucella
spp.Agent Specific Training
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/aspSlide2
Brucellosis Worldwide
Brucellosis can be found worldwideMore common in countries that do not have effective public health and domestic animal health programsMediterranean Basin (Portugal, Spain, Southern France, Italy, Greece, Turkey)MexicoSouth and Central AmericaAsiaAfricaCaribbean
Middle East
CDC
Slide3
Brucellosis in the United States
B.
abortus
–
present in wild elk and bison
B.
suis
– present in feral swine
B.
melitensis
– foreign animal disease
B.
canis
- signs of this disease are different in both genders of dogs
Rita
Traxler
, CDC
CDC Public Health Image Library #4876 http://phil.cdc.gov/phil/home/asp
CDC Public Health Image Library #6327 http://phil.cdc.gov/phil/home/aspSlide4
Sources of Human Infection in the U.S.
Ingestion of imported, unpasteurized dairy products.Contact with meat and other tissues from infected animalsLaboratory exposures - Aerosol exposure represents a significant risk*infectious dose as low as 10-100 organisms
CDC Public Health Image Library #12344, Dr. Michael Schwartz http://phil.cdc.gov/phil/home/aspSlide5
Brucella ssp.
Caution:
Brucella
species are highly pathogenic and have caused laboratory acquired infections. It should only be manipulated inside a Biological Safety Cabinet (BSC).
This is the most commonly acquired bacterial infection associated with clinical laboratory work.
Gathany
, James (Photographer). (2005). CDC Public Health Image Library, #8407. http://phil.cdc.gov/phil/home/asp. Slide6
Brucellosis Warning Signs
Communication between the laboratory and Physician/Epidemiologist is Critical!
Extremely variable
Fever, headache, chills
Body and backache
Intermittent, irregular fever – “Undulant fever”
Chronic fatigue syndrome-like symptoms
Arthritis
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CDC Public Health Image Library #13509 http://phil.cdc.gov/phil/home/aspSlide7
Brucella
spp.Specimen Sources
Bone marrow or whole bloodJoint or abdominal fluidSpleen, liver abscessesSerum
CDC Public Health Image Library #3438/Dr. Chen http://phil.cdc.gov/phil/home/aspSlide8
Brucella
spp.General Characteristics
Gram Stain Small, Gram-negative coccobacillusGenerally, faintly stainingSlightly larger than Francisella
tularensis
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Brucella
spp.
gram stain
CDC Public Health Image Library #1901 http://phil.cdc.gov/phil/home/aspSlide9
Brucella spp.
General Characteristics
Culture 48-72 hours SBA at 35ºC0.5 – 1.0 mm coloniesNon-hemolyticCO2 Enhancement/requirementSBA and CHOC supports growth
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B.
melitensis
mature culture
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/aspSlide10
Diagnostic assays for
Brucella spp.
Diagnostic
assay
Result
Gram stain
Tiny, faintly staining GNCB
Culture growth and morphology on solid medium
Non-pigmented, non-hemolytic, translucent, gray-white, pinpoint
and smooth
Oxidase
Positive
Urea
Strong Positive
Catalase
PositiveX&V factors Not requiredSatellite growthNegative
CDC Public Health Image Library – Image #12288
La’Vonda
Benbow, NCSLPH
La’Vonda
Benbow, NCSLPHSlide11
Brucella speciation