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Brucella  spp. Agent Specific Training Brucella  spp. Agent Specific Training

Brucella spp. Agent Specific Training - PowerPoint Presentation

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Brucella spp. Agent Specific Training - PPT Presentation

CDC Public Health Image Library 1928 httpphilcdcgovphilhomeasp Brucellosis Worldwide Brucellosis can be found worldwide More common in countries that do not have effective public health and domestic animal health programs ID: 745283

phil cdc public health cdc phil health public image library asp brucella http gov spp brucellosis gram photo laboratory delete stain text

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Presentation Transcript

Slide1

Brucella

spp.Agent Specific Training

CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/aspSlide2

Brucellosis Worldwide

Brucellosis can be found worldwideMore common in countries that do not have effective public health and domestic animal health programsMediterranean Basin (Portugal, Spain, Southern France, Italy, Greece, Turkey)MexicoSouth and Central AmericaAsiaAfricaCaribbean

Middle East

CDC

Slide3

Brucellosis in the United States

B.

abortus

present in wild elk and bison

B.

suis

– present in feral swine

B.

melitensis

– foreign animal disease

B.

canis

- signs of this disease are different in both genders of dogs

Rita

Traxler

, CDC

CDC Public Health Image Library #4876 http://phil.cdc.gov/phil/home/asp

CDC Public Health Image Library #6327 http://phil.cdc.gov/phil/home/aspSlide4

Sources of Human Infection in the U.S.

Ingestion of imported, unpasteurized dairy products.Contact with meat and other tissues from infected animalsLaboratory exposures - Aerosol exposure represents a significant risk*infectious dose as low as 10-100 organisms

CDC Public Health Image Library #12344, Dr. Michael Schwartz http://phil.cdc.gov/phil/home/aspSlide5

Brucella ssp.

Caution:

Brucella

species are highly pathogenic and have caused laboratory acquired infections. It should only be manipulated inside a Biological Safety Cabinet (BSC).

This is the most commonly acquired bacterial infection associated with clinical laboratory work.

Gathany

, James (Photographer). (2005). CDC Public Health Image Library, #8407. http://phil.cdc.gov/phil/home/asp. Slide6

Brucellosis Warning Signs

Communication between the laboratory and Physician/Epidemiologist is Critical!

Extremely variable

Fever, headache, chills

Body and backache

Intermittent, irregular fever – “Undulant fever”

Chronic fatigue syndrome-like symptoms

Arthritis

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CDC Public Health Image Library #13509 http://phil.cdc.gov/phil/home/aspSlide7

Brucella

spp.Specimen Sources

Bone marrow or whole bloodJoint or abdominal fluidSpleen, liver abscessesSerum

CDC Public Health Image Library #3438/Dr. Chen http://phil.cdc.gov/phil/home/aspSlide8

Brucella

spp.General Characteristics

Gram Stain Small, Gram-negative coccobacillusGenerally, faintly stainingSlightly larger than Francisella

tularensis

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Brucella

spp.

gram stain

CDC Public Health Image Library #1901 http://phil.cdc.gov/phil/home/aspSlide9

Brucella spp.

General Characteristics

Culture 48-72 hours SBA at 35ºC0.5 – 1.0 mm coloniesNon-hemolyticCO2 Enhancement/requirementSBA and CHOC supports growth

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B.

melitensis

mature culture

CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/aspSlide10

Diagnostic assays for

Brucella spp.

Diagnostic

assay

Result

Gram stain

Tiny, faintly staining GNCB

Culture growth and morphology on solid medium

Non-pigmented, non-hemolytic, translucent, gray-white, pinpoint

and smooth

Oxidase

Positive

Urea

Strong Positive

Catalase

PositiveX&V factors Not requiredSatellite growthNegative

CDC Public Health Image Library – Image #12288

La’Vonda

Benbow, NCSLPH

La’Vonda

Benbow, NCSLPHSlide11

Brucella speciation