CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp. Brucellosis Worldwide. . . Brucellosis can be found worldwide. More common in countries that do not have effective public health and domestic animal health programs. ID: 745283
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Presentations text content in Brucella spp. Agent Specific Training
Slide1
Brucella
spp.Agent Specific Training
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp
Slide2
Brucellosis Worldwide
Brucellosis can be found worldwideMore common in countries that do not have effective public health and domestic animal health programsMediterranean Basin (Portugal, Spain, Southern France, Italy, Greece, Turkey)MexicoSouth and Central AmericaAsiaAfricaCaribbeanMiddle East
CDC
Slide3
Brucellosis in the United States
B.
abortus
–
present in wild elk and bison
B.
suis
– present in feral swine
B.
melitensis
– foreign animal disease
B.
canis
- signs of this disease are different in both genders of dogs
Rita
Traxler
, CDC
CDC Public Health Image Library #4876 http://phil.cdc.gov/phil/home/asp
CDC Public Health Image Library #6327 http://phil.cdc.gov/phil/home/asp
Slide4
Sources of Human Infection in the U.S.
Ingestion of imported, unpasteurized dairy products.Contact with meat and other tissues from infected animalsLaboratory exposures - Aerosol exposure represents a significant risk*infectious dose as low as 10-100 organisms
CDC Public Health Image Library #12344, Dr. Michael Schwartz http://phil.cdc.gov/phil/home/asp
Slide5
Brucella ssp.
Caution:
Brucella
species are highly pathogenic and have caused laboratory acquired infections. It should only be manipulated inside a Biological Safety Cabinet (BSC).
This is the most commonly acquired bacterial infection associated with clinical laboratory work.
Gathany
, James (Photographer). (2005). CDC Public Health Image Library, #8407. http://phil.cdc.gov/phil/home/asp.
Slide6
Brucellosis Warning Signs
Communication between the laboratory and Physician/Epidemiologist is Critical!
Extremely variable
Fever, headache, chills
Body and backache
Intermittent, irregular fever – “Undulant fever”
Chronic fatigue syndrome-like symptoms
Arthritis
Delete text and place photo here.
CDC Public Health Image Library #13509 http://phil.cdc.gov/phil/home/asp
Slide7
Brucella
spp.Specimen Sources
Bone marrow or whole bloodJoint or abdominal fluidSpleen, liver abscessesSerum
CDC Public Health Image Library #3438/Dr. Chen http://phil.cdc.gov/phil/home/asp
Slide8
Brucella
spp.General Characteristics
Gram Stain Small, Gram-negative coccobacillusGenerally, faintly stainingSlightly larger than Francisella
tularensis
Delete text and place photo here.
Brucella
spp.
gram stain
CDC Public Health Image Library #1901 http://phil.cdc.gov/phil/home/asp
Slide9
Brucella spp.
General Characteristics
Culture 48-72 hours SBA at 35ºC0.5 – 1.0 mm coloniesNon-hemolyticCO2 Enhancement/requirementSBA and CHOC supports growth
Delete text and place photo here.
B.
melitensis
mature culture
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp
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DownloadNote - The PPT/PDF document "Brucella spp. Agent Specific Training" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Presentations text content in Brucella spp. Agent Specific Training
Brucella
spp.Agent Specific Training
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp
Slide2Brucellosis Worldwide
Brucellosis can be found worldwideMore common in countries that do not have effective public health and domestic animal health programsMediterranean Basin (Portugal, Spain, Southern France, Italy, Greece, Turkey)MexicoSouth and Central AmericaAsiaAfricaCaribbeanMiddle East
CDC
Slide3Brucellosis in the United States
B.
abortus
–
present in wild elk and bison
B.
suis
– present in feral swine
B.
melitensis
– foreign animal disease
B.
canis
- signs of this disease are different in both genders of dogs
Rita
Traxler
, CDC
CDC Public Health Image Library #4876 http://phil.cdc.gov/phil/home/asp
CDC Public Health Image Library #6327 http://phil.cdc.gov/phil/home/asp
Slide4Sources of Human Infection in the U.S.
Ingestion of imported, unpasteurized dairy products.Contact with meat and other tissues from infected animalsLaboratory exposures - Aerosol exposure represents a significant risk*infectious dose as low as 10-100 organisms
CDC Public Health Image Library #12344, Dr. Michael Schwartz http://phil.cdc.gov/phil/home/asp
Slide5Brucella ssp.
Caution:
Brucella
species are highly pathogenic and have caused laboratory acquired infections. It should only be manipulated inside a Biological Safety Cabinet (BSC).
This is the most commonly acquired bacterial infection associated with clinical laboratory work.
Gathany
, James (Photographer). (2005). CDC Public Health Image Library, #8407. http://phil.cdc.gov/phil/home/asp.
Slide6Brucellosis Warning Signs
Communication between the laboratory and Physician/Epidemiologist is Critical!
Extremely variable
Fever, headache, chills
Body and backache
Intermittent, irregular fever – “Undulant fever”
Chronic fatigue syndrome-like symptoms
Arthritis
Delete text and place photo here.
CDC Public Health Image Library #13509 http://phil.cdc.gov/phil/home/asp
Slide7Brucella
spp.Specimen Sources
Bone marrow or whole bloodJoint or abdominal fluidSpleen, liver abscessesSerum
CDC Public Health Image Library #3438/Dr. Chen http://phil.cdc.gov/phil/home/asp
Slide8Brucella
spp.General Characteristics
Gram Stain Small, Gram-negative coccobacillusGenerally, faintly stainingSlightly larger than Francisella
tularensis
Delete text and place photo here.
Brucella
spp.
gram stain
CDC Public Health Image Library #1901 http://phil.cdc.gov/phil/home/asp
Slide9Brucella spp.
General Characteristics
Culture 48-72 hours SBA at 35ºC0.5 – 1.0 mm coloniesNon-hemolyticCO2 Enhancement/requirementSBA and CHOC supports growth
Delete text and place photo here.
B.
melitensis
mature culture
CDC Public Health Image Library #1928 http://phil.cdc.gov/phil/home/asp
Slide10Diagnostic assays for
Brucella spp.
Diagnostic
assay
Result
Gram stain
Tiny, faintly staining GNCB
Culture growth and morphology on solid medium
Non-pigmented, non-hemolytic, translucent, gray-white, pinpoint
and smooth
Oxidase
Positive
Urea
Strong Positive
Catalase
PositiveX&V factors Not requiredSatellite growthNegative
CDC Public Health Image Library – Image #12288
La’Vonda
Benbow, NCSLPH
La’Vonda
Benbow, NCSLPH
Slide11Brucella speciation