Mary Mitchell JC Achenbach Sean Tibbetts Lee Ellis What are Zebrafish 2 Danio rerio part of the minnow family Small tropical freshwater fish Native to South Asia 15 2 inches ID: 742025
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Slide1
Zebrafish feed model: Applications in aquaculture nutrition research
Mary Mitchell, JC Achenbach, Sean
Tibbetts
, Lee EllisSlide2
What are Zebrafish?
2
Danio
rerio
– part of the minnow familySmall tropical freshwater fishNative to South Asia~ 1.5 – 2 inchesPopular aquarium fishSlide3
Zebrafish as a research model
Broadly recognized research
models
Low-cost
husbandryProlific breeders and quick generation timesTransparent embryosSequenced genomeEstablished transgenic lines3Slide4
Zebrafish as a model for aquaculture nutrition researchUlloa
et.al,
2014
High costs of testing novel compounds Reduced feed requirementsDiets effect on molecular mechanisms RNA sequencingNutritional genomics In vivo response monitoring with fluorescently labelled proteins
4Slide5
Zebrafish Feed ModelDeveloped to assess the efficacy of various feed additives and diets on
zebrafish
Two standardized purified diets
Control vs.
Astaxanthin + diet (80mg/kg feed)Effects on growth and development Development of Standardized measurementsUptakeTissue analysis Whole
body and tissue specific
5Slide6
Zebrafish Feed ModelImportance placed on reducing inter and
intra-tank
variability
Minimum densities
while rearing prior to trial25 larval fish (5dpf-30dpf) per basket – 2 baskets/3L tank15 fish/3L tank >30dpf Highly selective of fish for trialSize and healthStarted with 130
AB
line zebrafish
65 fish per diet
5 tanks with 13 fish each
6Slide7
Zebrafish Feed Model
Trial ran ~ 8 weeks
Started feeding fish trial diets at 38dpf till 90dpf
One week to acclimate to purified diets
Fed twice daily to satiation5 sampling events Lethal and non-lethal sampling events 13 fish per diet randomly sampled each eventAcquired weight and length measurementsDissection – brain, liver, gut, eyes, gills, spleen, kidneys, reproductive organs
7Slide8
Sampling Procedures
8
38dpf 90dpfSlide9
Results
Measured weight, standard length, and feed intak
e
9Slide10
Results10
Minimal feed requirements
~0.3g feed/fish for ~8 week trialSlide11
In conclusion
11
Zebrafish feed model could provide a low cost alternative to some current aquaculture feed trials
Develop make and test diets in house
Low husbandry and feed costs
Low degree of variance between clutches allows for identifying small differences in growth between groups
Serve as a pre-cursor to larger scale trials with intended speciesSlide12
QUESTIONS?
12