PPT-PAG 7.2 Dilution plating to determine microbial density in liquid culture

Dilution plating to determine microbial density in liquid culture Aim To find the density of viable bacterial cells in two cultures of Bacillus subtilis by dilution plating Scientists use a number of different methods to determine the number of microorganisms that are present in a given population. Get the tools:. Lab book. CPS Remote. Calculator. density: . the amount of matter (mass) in a given unit volume. .. mass: . the measure of the amount of matter an object contains. . viscosity. : the resistance of a liquid to flow (. Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER . 8. Preservation of Microorganisms Collections. 2. Culture Preservation. Streptomyces aureofaciens NRRL 2209 was the first microorganism deposited in a culture collection in support of a microbially based patent application.. The . concentrationof. matter in an object is known as . density. .. Computing Density. . Density = . mass (. g. ) . volume (cm. 3. ). DETERMINE VOLUME. :. One way to determine volume is to use a graduated cylinder to determine . Liquid Layers. If you pour together liquids that don’t mix and have different densities, they will form liquid layers.. The liquid with the . highest density. will be . on the bottom. .. The liquid with the . Zumdahl. 7. th. . ed. 53. . A material will float on the surface of a liquid if the material . has a . density less than that of the liquid. Given that the density of . water is . approximately 1.0 g/mL, will a block of material having . Objects that sink . in water are more dense than water. ; objects . that float are less dense.. Learning Goal . . Calculate the density of a substance; use the density to calculate the mass or volume of a substance.. Introduction. You’ve got larval fish!! Good job!!. Now what?? . If you’ve researched then it shouldn’t be a big deal, because you’re ready to feed those little critters!. Mostly food for larval fish is size dependant. If they can get it down and it doesn’t damage their gut lumen, then it might be a good food. . Introduction. You’ve got larval fish!! Good job!!. Now what?? . If you’ve researched then it shouldn’t be a big deal, because you’re ready to feed those little critters!. Mostly food for larval fish is size dependant. If they can get it down and it doesn’t damage their gut lumen, then it might be a good food. . Microbial growth is the increase in number of cells, not cell size. Microbial Growth. The Requirements for Growth: . Physical Requirements. Temperature. Minimum growth temperature. Optimum growth temperature. CPS Remote. Calculator. density: . the amount of matter (mass) in a given unit volume. .. mass: . the measure of the amount of matter an object contains. . viscosity. : the resistance of a liquid to flow (. Musafer. PhD. Microbial growth defined as an increase in cellular constituents resulting an increase in a microorganism size, population number or both.. 1-Lag phase. When M.O are inoculated into fresh culture medium, will not reproduce immediately, therefore this period is called the lag phase. . 1. Microbial survival strategies. 2. Microbial growth. 3. Effects of the environment. 4. Microbial cultures. 5. Growth measurements. 6. Antimicrobial agents. 7. Antibiotics. 1. . Microbial. . survival. in water are more dense than water. ; objects . that float are less dense.. Learning Goal . . Calculate the density of a substance; use the density to calculate the mass or volume of a substance.. Densities of Common Substances. biotechnology.important. branch of Microbiology where economically valuable products are produced from cheaper raw materials by using large culture of . m.o.. Up stream and Downstream process.. Screening-Definition..