PPT-Enzymes in UniProtKB Webinar

27 th September 2017 wwwuniprotorg The two sides of UniProtKB curated by experts data from scientific papers annotation of sequence features collates isoforms in one entry annotation from rule systems incl expertcurated rules. Understanding:. Enzymes have an active site to which specific substrates bind. Enzyme catalysis involves molecular motion and the collision of substrates with the active site. Temperature, pH and substrate concentration affect the rate of activity of enzymes. ch. 12. The protein catalyst. Targets:. Describe the relationship between substrate and an active site in enzymatic reactions. Identify the role of coenzymes and enzymatic reactions. List factors that affect enzymatic activity. Reactions. Most reactions take place in a number of steps which need to be carefully controlled if the cell is to function properly. Enzymes are the most important controllers of cellular reactions. Catalysts. Apoenzymes. , . Holoenzyme. , . Abzymes. , . Synzyme. , . Ribozyme. , . Extremozyme. , Historical Development in . Enzymology. , . Characteristics of enzymes, Enzyme Vs Chemical Catalysts. , Enzyme mechanism & Activation Energy, Enzyme Specificity, Fischer and . Presented by Fotios Patsalis . Bio . 446/546 FALL 2016. M/F 13.00-15.50h,. Castetter. . Hall 37. Restriction Enzymes: Molecular Scissors. Restriction enzymes (. endonuleases. ) are specific endonucleases which . Enzymes speed up chemical reactions.. Everything that happens in a cell. Growth. Reproduction. Interaction with the environment. Response to stimuli. Is the result of a chemical reaction.. A chemical reaction is a change in the arrangement. E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. IMPORTANCE. OF ENZYMES. Enzymes are biological catalysts that:. . I. ncrease. . reaction rates . by lowering the amount of energy needed for the reaction to occur. 100-1000x . faster. !!!. Activation Energy. protein. !. Enzymes are . Biological . catalysts . that . increase. . the rate of metabolic reactions.. Since enzymes speed up chemical reaction rates, what are they called?. Catalyst- . A substance . frequency (number of occurrence in the data set). The vast majority of closed sets Georgetown University Medical Center. University of Delaware. Youth Bioinformatics Symposium 2016. Cecilia . Arighi. . Hongzhan. Huang. Karen Ross C.R. . . Vinayaka. . Cathy Wu. : An enzymes that breaks down nucleic acid molecule by breaking the phosphodiester bonds at end 3. / . or 5. /.. Endonucleases. : . An Enzyme that hydrolyzes internal phosphodiester bonds in a polynucleotide chain or nucleic acid molecules.. Enzymes. Back when we discussed proteins, we learned that one critical function of protein was to speed up chemical reactions.. Enzymes. are the proteins that increase the rate of chemical reactions. To the penny placed on your desk.. Observe the penny but don’t touch it. What happens?. Explain why. 2. Give the penny a . little . push. What happened?. Why did the penny move the second time and not the first time?.