PPT-Measuring Apoptosis using Flow Cytometry

Rich Hastings KUMC Flow Cytometry Core Lab 3901 Rainbow Boulevard Kansas City KS 66160 9135880627 rhastingskumcedu httpwwwkumceduflow Cell Membrane Cell membrane integrity . FITC. PE. Median. % of Grandparent. % of Parent. Mode. Geometric mean. CV. % Total. Data Analysis. Mean. Experimental Design. Instrumentation. Analysis. Presentation. Sample Procurement. Sample preparation. James . Marvin. Director, Flow . Cytometry. Core Facility. University of Utah Health Sciences Center. Office 801-585-7382. Lab 801-581-. 8641. Seventeen-. colour. flow cytometry: . unravelling. the immune system. Dr. Tania A. . Shakoori. Apoptosis. Apoptosis.  . -. programmed . cell . death. 3 stages. Initiation . (depending on where the . the. signal comes from). Extrinsic. Intrinsic. Execution. Phagocytosis. Cytometry. 101: the “what, why and how”. IMMU7040 - immunological Methodology . February 18, 2014. Christine Zhang, PhD. Faculty of Medicine . University of Manitoba. Presentation Outline. Basic Concept of Flow . Examples of apoptosis induction with different kinetics measured by competing technologies such as TUNEL or Annexin . V. Slow induction of apoptosis. Rapid induction of apoptosis. The time point of the measurement is crucial:. ested Com p anion Products C atal og m r N o n e S 554656Stain Buffer (FBS)500 mL(none)554657Stain Buffer (BSA)500 mL(none)556454Annexin V Binding Buffer, 10X concentrate50 mL(none)556419FITC Annexin Andrology. lab. Yazd Reproductive Sciences Institute . بسم الله الرحمن الرحیم. Introduction into cell death. In humans. , . the rate of . cell growth . and . cell death . is balanced to maintain the weight of the body. . Lessons leaned. Apoptoti. c process. definition . A . programmed cell death process which. . begins. . when. . a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathways) which typically lead to rounding-up of the cell, retraction of . Director, Flow . Cytometry. Core Facility. University of Utah Health Sciences Center. Office 801-585-7382. Lab 801-581-8641. jmarvin@cores.utah.edu. ****Lab= . Wintrobe. . Bldg. Rm 221****. https://utahflowcytometry.wordpress.com/presentations/. By: Michelle Rivera, . eSmirna. . cantu. , Alexa Campos. What are pesticides?. Pesticides is a generic term that many chemicals fall under. . Brief functions of pesticides. . List of pesticides used. . Novus-lu-2945 novusbio.com novusbio.com Apoptosis is derived from the Greek pre�x apo-, meaning “away, off”, and the word ptosis, meaning “falling, a fall”. IntroductionApoptosis Si The �ow cytometer was developed in the 1970’s and rapidly be by the HIV pandemic and a plethora of discoveries in hematology, specialized �ow cytometers for use in the clinica Apoptosis, often referred to as programmed cell death, is a tightly regulated and fundamental biological process that plays a crucial role in maintaining tissue homeostasis, development, and defense against various cellular stressors.. LEC.5. 3. Evasion of apoptosis:. Normally, . the mechanism of . apoptosis is mediated by many genes, & achieve by two phases. . Initiation phase. , which is mediated by . TNF- Fas receptor . & inhibited by FLIP protein..