Scientific Codirector International Project KOMP Knock Out Mouse Project Trans NIH initiative aims to generate a comprehensive and public resource of murine ESCs containing a null mutation in every gene in the mouse ID: 920138
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Slide1
KOMP and KOMP2
Trish Labosky
Scientific Co-director
Slide2International Project
Slide3KOMP
Knock Out Mouse Project
Trans
-NIH
initiative, aims
to generate a comprehensive and public resource of murine ESCs containing a null mutation in every gene in the mouse genome.
Slide4KOMP Goals
U
se
gene targeting
to
generate null alleles, marked with a high utility reporter, preferably in C57BL/6;
Develop improved C57BL/6 ES cells capable of robust germline
transmission, to be used in a high throughput pipeline in generating this resource;
Support
a repository to
house
the products of this
resource,
additional ‘repatriation' effort to bring
in 1000 of the existing high priority mouse knockouts; Implement a data coordination center to make the status and relevant data of the production effort available to the research community.
1.
Regeneron
Pharmaceuticals
2. Children's Hospital Oakland Research Institute (CHORI), School of Veterinary Medicine, University of California, Davis (UC Davis) and the
Wellcome
Trust Sanger Institute
.
3. The Jackson Laboratory.
Slide5www.knockoutmouse.org
Slide6Additional KOMP mice
NIH awarded
contracts that gave academic researchers access to a set of
well-characterized
knockout mouse lines
from Deltagen and Lexicon
Genetics. ~ $11 million for ~250
lines of these micehttp://www.nih.gov
/science/models/mouse/
deltagenlexicon
/
list.html
Slide7http://
www.nih.gov
/science/models/mouse/
deltagenlexicon
/list.html
Slide8Slide9Slide10Nature (2011) 474, 262.
Vast majority of mice are from the
Wellcome
Trust
Slide11Have targeted more than 9000 genes.
Null allele
Null allele
with reporter
Conditional allele
Slide12What if you don’t know which genes are involved in your biological system/question?
Slide13KOMP2: Newly Funded
Harwell
Jax
Baylor
UC Davis
Slide14Phenotyping Platform
Slide15Slide16Slide17Slide18Fees
MRRCC
KOMP Davis
Slide19What to do when you find a line of interest:
You submit MTA, order ESCs (we recommend the “PREMIUM ESC PACKAGE”, $2801), and have them sent to us.
Additional expenses:
ESC expansion,
freezeback
, prep for path screening $1000 / clone
ESC Karyotyping (if needed) $350 / clonePath screening by DAC $350 / cloneESC prep / injection day $450
Injection into Albino BL6 / day $2600Injection into BL6 / day $1650
Slide20TMESCSR Website
http://
www.vanderbiltresearch.org
/community/profile/id/8