PDF-In Proc. of the 1990 ACM Intl. Workshop on Timingissues in the Specifi

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ThisworkwassupportedbANC ChannelsSignalsEvtsandActionsInourmodeltimeisameasurableconuousinnitelydivisiblequanerepresentanpointintimebyanonnegativerealTimeinalsaredenedbyspecifyin

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In Proc. of the 1990 ACM Intl. Workshop on Timingissues in the Specifi: Transcript

ThisworkwassupportedbANC ChannelsSignalsEvtsandActionsInourmodeltimeisameasurableconuousinnitelydivisiblequanerepresentanpointintimebyanonnegativerealTimeinalsaredenedbyspecifyin. Proc.Natl.Acad.Sci.USA87(1990)6389Corey-Bransona-helixcannotberepresentedwithperfectaccuracyonanylatticeoflowcoordinationnumber,butthehelicesobservedincrystallographydatabasesofrealpro-teinsarelikewis Proc.Natl.Acad.Sci.USA87(1990)wereprepared(25),andtheDNAinsertwasexcisedwithEcoRI,isolatedbylowmeltagarosegelelectrophoresis,andrandom-primedforexpressionanalysis.Theexpressionlev-elsandsizesofthemRNA Proc.Nati.Acad.Sci.USA87(1990)2527A4T4FIG.2.StereoscopicdifferenceFourierelectrondensitymapatthepositionofthemissingphosphateinbothA4andT4models.ofotherunlikelycombinationssuchasadodecamerduplexwithfo Proc. VIth Intl. Congress on Hazelnut Eds.: J. Tous, M. Rovira & A. Romero Acta Hort. 686, ISHS 2005 Minimum air temperatures periodically occur in the northeastern U.S. with potential to cause exte MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Proc.Natl.Acad.Sci.USA88(1991)5593Table1.YeaststrainsNuclearMitochondrialStraingenotypegenotypeRef.947aade2ura3-52p09ID41-6aadellysi15161p+15C1036mit-;COXJintron116splicing-defectivepointmutant(trans- Proc.Natl.Acad.Sci.USA91(1994)9991pooled;theproteinswereprecipitatedwith(NH4)2SO4anddesaltedasdescribedabove,exceptthattheproteinswereelutedinavolumeof3.5mlusingabuffercontaining50mMNaCland50mMTris-HC Proc.Natl.Acad.Sci.USA89(1992)8493stringencyhybridizationwithAcZ1,ahumanTALIcDNAfragment(15).Eachhybridizingphageclonewasthenexam-inedbynucleotidesequenceanalysisforthepresenceofanopenreadingframethat 6838PlantBiology:Staswicketal.2520EE154-Pcm-100050o-8-7-6-5-4Log1oMeJAconcentration(M)FIG.1.GrowthinhibitionofprimaryrootsbyMeJA.Rootlengthofjarlandwild-type(wt)plantswasmeasured9daysafterplanting.Bar 6546BiochemistryAkimaruetalpH75containing150mMNaCIfrozenthawedandsonicatedasdescribed8InVitroTranscriptionandTranslationInvitrotranscrip-tionoftheompA-D26andompAgeneswasperformedasdescribed18Thetransl ProcNatLAcadSciUSA921995pDUCA721byeliminatingtheSalIsitebetweenoriTandoriWAnabaena7120genomicDNAwaspartiallydigestedwithCpfItoyieldfragmentsthataveraged20-40kbinsizeandthendephosphorylatedVectorarmsea ProcNatlAcadSciUSA8919928449perreaction201lAfteraninitialdenaturationof7minat940Candtheadditionof1unitofTaqDNApolymeraseat40C27cyclesofamplificationeachconsistingof30secat940C45secat50Cand120secat720C

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