PPT-Unit 2: The Genome Chapter 6 - Polymerase Chain Reaction

Figure 601 Polymerase Chain Reaction PCR During PCR two primers anneal to complementary sequences at either end of a target sequence on a piece of denatured sample DNA DNA polymerase synthesizes a complementary strand of DNA from the primers resulting in two new strands of DNA In further cycles the newly made DNA molecules are denatured primers are annealed and DNA polymerase copies the target regions resulting in multiple copies of the original target sequence. (PCR). PCR. PCR produces billions of copies of a specific piece of DNA from trace amounts of starting material. (i.e. blood, skin cells, bone). Allows scientists to isolate pure quantities of specific DNA sequences. & 9. Polymerase Chain Reaction (PCR). General Genetics. Objectives:. 1.Introduce the students to the preparation of the . PCR. reaction.. 2.Examine the . PCR. products on . agarose. gel electrophoresis.. & 9. Polymerase Chain Reaction (PCR). General Genetics. Objectives:. 1.Introduce the students to the preparation of the . PCR. reaction.. 2.Examine the . PCR. products on . agarose. gel electrophoresis.. By: Savana Canary and Kathryn Wolfe. What is it?. Polymerase Chain. Reaction (PCR) is when . you amplify the number. of copies of a specific. region of DNA, in order . to produce enough DNA. it be adequately tested.. by: Keeanna Wolcik and Gabbie Hahn. Key Terms. denature ~ in the DNA sense, unwind. anneal ~ bind. primers ~ strand of nucleic acids that are used as a starting point for DNA synthesis. Taq polymerase ~ an enzyme that synthesizes polymers; from bacteria that live in hot springs; . Nahla . Bakhamis. Multiple copies of specific DNA . sequences;. . ‘Molecular Photocopying’ . Polymerase Chain Reaction. 1983;. In . vitro. enzymatic amplification of specific DNA sequences from . "molecular photocopying" . It’s fast, inexpensive and simple . Polymerase Chain Reaction. Amplifying DNA . in Vitro. : The Polymerase Chain Reaction (PCR). The . polymerase chain reaction, PCR. , can produce many copies of a specific target segment of DNA. Suggested Reaction ProtocolT7 RDNA Polymerase will incorporate many di26erent 2-deoxy NTPs and 2-modi31ed NTPs except for 2-O-methyl UTP or CTP 29e e25ciency of incorporation yield and optimal reactio CULTURE INDEPENDENT DIAGNOSTIC TESTING CIDTLook at the pathogens bacteria virus fungus under a microscopeBacteria often look alike even when stainedSometimes fairly specific Syphilis treponema Gram-ne O. T. N. N. R. H. N H. O. C. R. N. N. N. N. H. H N. A. G. R. N. N. N. H N. O. H N. H. U. OH. CH2. O. OH. OH. O. O P . O. O. O P . O. O. O P . O. OH. Ribose. Deoxy. Ribose. Morré SA, Spaargaren J, Fennema J, de Vries H, Coutinho RA, Peña A. Real-time Polymerase Chain Reaction To Diagnose Lymphogranuloma Venereum. Emerg Infect Dis. 2005;11(8):1311-1312. https://doi.org/10.3201/eid1108.050535. Figure 8.01. Sequencing—Fragments of All Possible Lengths. During chain termination or . Sanger sequencing. , the target DNA fragments are copied millions of times, but each copy ends at a different nucleotide position. These subsets of fragments end with a fluorescently-labeled nucleotide that reveal the identity of the final base. The final sequencing data are a series of fluorescent peaks that correspond to the original template . Key points:. Polymerase chain reaction. , or . PCR. , is a technique to make many copies of a specific DNA region . in vitro.  (in a test tube rather than an organism).. PCR relies on a . thermostable. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.