Simplify smallscale isolation of total RNA Conserve resources by using a single kit for multiple applications  eliminating the need to use several kits from other suppliers
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Simplify smallscale isolation of total RNA Conserve resources by using a single kit for multiple applications eliminating the need to use several kits from other suppliers

Rely on the kits integrated oncolumn DNasetreatment step to degrade genomic DNA Obtain high sensitivity reproducibility and specificity in quantitative RTPCR and other applications Figure 1 Choose the High Pure RNA Isolation Kit to rapidly isolate i

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Simplify smallscale isolation of total RNA Conserve resources by using a single kit for multiple applications eliminating the need to use several kits from other suppliers




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Presentation on theme: "Simplify smallscale isolation of total RNA Conserve resources by using a single kit for multiple applications eliminating the need to use several kits from other suppliers"— Presentation transcript:


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Simplify small-scale isolation of total RNA Conserve resources by using a single kit for multiple applications - eliminating the need to use several kits from other suppliers. Rely on the kit's integrated on-column DNase-treatment step to degrade genomic DNA. Obtain high sensitivity, reproducibility, and specificity in quantitative RT-PCR and other applications (Figure 1). Choose the High Pure RNA Isolation Kit to rapidly isolate intact total RNA from a broad range of research sample materials, including cultured cells, mammalian blood, white blood cells (WBCs), yeast, and

bacteria. Recover highly pure, concentrated RNA (in 50 l) from one sample in 25 minutes, and process multiple samples in 45 minutes with a straightforward workflow. Produce high-quality template for direct use in cDNA library construction, RT-PCR, qRT-PCR, northern blotting, differen tial display, nuclease protection assays, primer extension, RACE, and in vitro translation. in vitro Enhydra lutris
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Published by Protocol for isolating total RNA from up to 10 cultured cells. View detailed procedures for other sample materials in the pack insert at

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"EEoM&MVUJPO#VGGFSUPUIFVQQFSSFTFSWPJSPG n$FOUSJGVHFUIFUVCFBTTFNCMZGPSNJOBU o$GPSMBUFSBOBMZTJT For more information about the High Pure RNA Isolation Kit and other products for nucleic acid isolation and purifica tion, visit Disc ard flowthrough Harv est 10 6 cultu re d cells and r esuspend cell pelle t ix well and pipet th ample into the

upper re servoir of the High Pu re F ilter T ube assembl y Centrifuge for 15 s at 8 ,000 x Add 4 00 l Ly s is/ Binding Buffe r Disc ard flowthrough Centrifuge for 15 s at 8 ,000 x Add 5 00 l W ash Buffer I Centrifuge for 2 mi n at max. speed (13,000 x g ) Centrifuge for 1 mi n at 8 ,000 x Disc ard flowthrough and Collection T ube Pu rified RN A Disc ard flowthrough Add 200 l Wa sh Buffer II Add 50 100 l Elutio n Buffe r Add 200 l PBS I ncubate for 15 mi n at +15 to +2 Fo r each s ample add ixture of 90 l D Nase I I ncubation Buffer and 10 l D Nase I Centrifuge for 15 s at 8 ,000 x Add 5 00 l

Wa sh Buffer II (S. cerevisiae) (E. coli) (B. subtilis)