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the Genetics Society Manuscript received 17 1993 sufficient overexpres


157 except 157 except 157 except Mutant recF Alleles 645 TABLE 2 Plasmids constructions and tecF alleles recF Allele Plasmid plasmid Primer Structural Expression resistance Ancestral Drug Reference

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Document on Subject : "the Genetics Society Manuscript received 17 1993 sufficient overexpres"— Transcript:

1 the Genetics Society Manuscript received
the Genetics Society Manuscript received 17, 1993 sufficient overexpr

2 ession 157 except 157 except 157 except
ession 157 except 157 except 157 except Mutant recF Alleles 645 TAB

3 LE 2 Plasmids, constructions, and tecF
LE 2 Plasmids, constructions, and tecF alleles recF Allele Plasmid

4 plasmid Primer Structural Expression re
plasmid Primer Structural Expression resistance Ancestral Drug Refer

5 ence pJC876 pJKKmf(-) pSE200 pSJS 140 pS
ence pJC876 pJKKmf(-) pSE200 pSJS 140 pSJSl59 ~sJS237~ pSJS530b pSJS6

6 0gc pSJS6 1 3d ~SJs622~ pSJS67Of pSJS67W
0gc pSJS6 1 3d ~SJs622~ pSJS67Of pSJS67W pSJS680h pSJS687' pSJS68tV p

7 SJS693" pSJS893' ~sJS894~ DUCl18 143 Non
SJS693" pSJS893' ~sJS894~ DUCl18 143 None None + + + pSJS 159 prSJS4

8 1 + pSJS159 prSJS44 + + 41 14 + 4114 p
1 + pSJS159 prSJS44 + + 41 14 + 4114 pSJS6 13 prSJS56 4101 4101 pSJ

9 S6 13 prSJS6 1 4104 4104 14? None + None
S6 13 prSJS6 1 4104 4104 14? None + None None + + + 4105 41 13 4105,

10 4113 4105, 4113 4105, 4113 4105,4113
4113 4105, 4113 4105, 4113 4105,4113 4105,4113 alleles: structura

11 lly neutral mutations designed aga aga m
lly neutral mutations designed aga aga mutant tcg ccg ggc ttc aaa 145

12 856 gga 922 leu asp tgg..42n..tga aga a
856 gga 922 leu asp tgg..42n..tga aga ala trp arg pro trp and SHERR