of Invert Sugar using Lentikats B iotechnology Content Our aim What is Lentikats Biocatalyst Production of Lentikats Biocatalyst Benefits of Lentikats Biotechnology Lentikats Biocatalyst in ID: 312687
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Slide1
Production
of Invert Sugar using
Lentikats B
iotechnologySlide2
Content
Our
aim
What is Lentikats Biocatalyst
Production of Lentikats Biocatalyst
Benefits of Lentikats Biotechnology
Lentikats Biocatalyst in
Food Industry
Our guarantees
Our partners
ContactsSlide3
LentiKat’s a.s.
in Food Industry
LentiKat
’s a.s. follows up on nine year of research and development of Lentikats Biotechnology.
Our production know-how for commercial use of our
Biocatalyst and for its industrial applications is patented internationally.Our main focus is in intensification of production processes that leads to:higher process yieldsenhanced process stability,Increased revenue,Reduction of environmental footprint.
Biotechnologies
are
chang
ing
the world
…Slide4
Lentikats Biotechnology
Unique shape of the matrix and original immobili
s
ation procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a hydrogel porous carrier from polyvinyl
al
cohol (PVA). The advantages of PVA matrix are:excellent physical and mechanical characteristics that result in a long-term mechanical stabilityhardly biodegradableno toxic effectsLentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly.
With biotechnologies to
a better future
…Slide5
How does the Lentikats Biocatalyst work?
A maximum surface in combination with the minimum volume of Lentikats Biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.Slide6
Why
polyvinylal
c
ohol?
It is an inexpensive material
Encapsulation takes place under optimal, cell-sparing conditions
It allows a production of hydrogel with excellent mechanical propertiesIt does not have any side effects on fermentationIt offers high capacity for the cell loadingThe process is stable and easy to controlIt is a non-toxic material
Biotechnologies
are
chang
ing
the world
…Slide7
How is Lentikats Biocatalyst produced
?Slide8
L
entikats
Biocatalysts
Biocatalyst Types for Use in Lentikats Encapsulation:
Free Cells
dead cells viable, non growing cells viable and growing cellsFree or Immobilised Enzymes.Lentikats Biotechnology allows to immobilise any type of enzyme The size of Lentikats Biocatalyst pores varies depending on the size and diameter of the Biocatalyst due to:PVA additive type (size of macromolecule)additive concentrationbiomass concentrationSlide9
L
enti
k
ats
Biocatalysts
Manufacturing PlantSlide10
Laboratory Units for Encapsulation
For low-capacity production of encapsulates
(especially for research purposes), a laboratory device is availableSlide11
What are the benefits?
Higher reaction rates
Reduction of reaction tanks’ volume
Operational costs savings (electricity, heat)
Lower production of waste and by-products
Environmentally friendly disposal
Matrix containing high concentration of biomass
Higher yields (in comparison to conventional technologies using free biomass)
Possibility of repeated
usage
Operational costs savings
Enhanced resistance of modified and immobilised biomass
Higher process stability
Easy separation of biomass from reaction media
Reduced costs for downstream processes (separation)Slide12
..and also…
Shortening of the reaction times
Much higher yields
in comparison to conventional technologies
(up to 5 – 15 % higher)Repetitive use of the Biocatalyst, easy separation from media (sieve separator)Higher productivity of the BiocatalystLower risk of contamination during the production process
High stability and easy control
of production process
Biotechnologies are changing the world…Slide13
Technological set-up
- inlet of
reaction media
-
filling of Lentikats Biocatalyst
-
medium with Lentikats Biocatalyst - stirrer - sieve separator - outlet of separated media - outlet of Lentikats Biocatalyst
Slide14
Lentikats
and food industry
We currently offer solutions for the following processes:
production of lactose free milk
Lentikats Biocatalyst with β-galaktosidase activity
production of invert sugar Lentikats Biocatalyst with invertase activitywhey processingLentikats Biocatalyst with β-galaktosidase activity for: production of D-galaktose from whey lactose (pharmaceutical quality)production of galacto-oligosaccharides from whey lactoseproduction of special syrupsLentikats Biocatalyst with invertase activity for production of glucose-fructose syrupsLentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar and starch-based materials or glucose syrups from maltotriose or maltose syrupSlide15
Lentikats
and food industry
Following solutions are currently under development and testing
:
production of organic acids
Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic acid from lactose, glucose and sucroseLentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production of gluconic acid from sucrose and glucoseLentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for production of itaconic acid from sucrose and glucose
Biotechnologies are changing the world…Slide16
Invert Sugar in Food Industry
Sucrose source
–
sugar beat
,
sugar cane
Mixture of glucose and fructose (sucrose hydrolysis)Invert sugar as a product:composition 33% sucrose, 35% glucose and 32% fructose » composition 4%
sucrose
, 51% glucose a
nd 45% fru
ctose
invert sugar is more expensive than syrup
»
invert sugar is sweeter and contains also other monosaccharides apart from sucrose
large variety of applications: kids food, pastry, soups and sauces, candy and sweets, fruit drinks, dairy products.Slide17
Invert sugar in Distilleries
Sucrose source
–
sugar beat
,
sugar cane
Mixture of glucose and fructosemolasses – benefits of sucrose hydrolysisa longer shelf-life » possibility of a year-round operation for molasses distilleriesa lower water binding capacity – possibility to a achieve higher thickness prior to storage (80%)
»
lower demands for storage space and transportationSlide18
Lentikats Biotechnology and production of invert sugar
Lentikats Biocatalyst with invertase activity
=
immobilised saccharase (β-D-fructofuranosidase)
saccharase source –
Baker’s yeast (Candida utilis)invertase activity 600 - 1000 g sucrose/kg LB/hroptimum temperature 30 - 35°Coptimum pH 4.
5 – 5
.0
operational stability
600 – 1000 hodin
18Slide19
Lentikats Biotechnology and production of invert sugar
The active compound is
immobilised invertase for sucrose hydrolysis
to glucose and fructose.
- Standard activity assay: batch teststandard sucrose solution with the concentration 100 g/ltemperature
30°C
pH 4
.5 (10 mM
acetate buffer
)
conversion time 50 min
conversion rate
100%Slide20
Case studiesSlide21
1)
Hydrolysis of sucrose solution
Input parameters
sucrose concentration
450 g/l pH = 4.5 temperature = 30°C batch operation mode solution volume: 2 222 m3
Lentikats Biocatalyst
activity
1000
g
SUC
/kg LB
/hr
stability
900 hr
amount
1
ton
Outlet
concentration
:
sucrose
10 g/l
glucose
220 g/l
fructose
220 g/l
volume
2 222
m
3
Invert sugar production
amount
1 00
0 000 kg
14 EUR
/m
3
solution
0
.03
EUR
/kg
invert sugar
Invert sugar productionSlide22
2) Hydrolysis of sucrose solution
Input parameters
sucrose concentration
450 g/l
pH = 4.5 temperature = 30°C batch operation mode Solution volume: 2222 m3
Lentikats Biocatalyst
activity
1000 g SUC/kg
LB
/hr
stability
1000 hr
amount 1 ton
Output
concentration
:
sucrose
10 g/l
glucose
220 g/l
fructose
220 g/l
volume
2 222
m
3
Ethanol production
volume
615 333
litres
a.a.
14 EUR
/m
3
solution
0.05
EUR
/
litre
a.a.
Ethanol productionSlide23
3) Molasses distillery
Input parameters
sucrose concentration
120 g/l pH = 4.5 temperature = 30°C continuous operation mode molasses volume: 8 333 m3
Lentikats Biocatalyst
a
c
tivit
y
1000 g SUC
/kg LB/hr
stability
1000 h
r
amount 1 ton
Output
concentration
:
sucrose
30 g/l
glucose
45 g/l
fructose
45 g/l
volume
8 333
m
3
Ethanol production
volume
629 167
litres
a.a.
4
EUR
/m
3
molasses
0.05
EUR
/litr
e
a.a.
Ethanol productionSlide24
Summary
–
application of 1 ton of LB
Sucrose solution
– 450 g/l
sucrose
Volume of solution processed
[m
3
]
Ethanol produced
[l a.a.]
Price
[EUR
/m
3
SUC solution]
Price
[
Kč/litr a.a.
]
1 ton LB
2222
615 333
14
0.05
Molasses
– 120 g/l
sucrose
Volume of molasses processed
[m
3
]
Ethanol produced
[l a.a.]
Price
[EUR
/m
3
molasses]
Price
[
Kč/litr a.a.
]
1 ton LB
8333
629 167
4
0.05Slide25
I
nvestment and
I
nvestment
R
eturn
GuaranteesYou can easily verify our great results by testing our Biocatalyst in your or other facilities with the material of your choice.Sample of Lentikats Biocatalyst free of chargeOur company provides assistance and full technical support. Our company offers a long-term cooperation to companies, whose main concerns are
reduced investment costs
and maximal process profitability.
Biotechnologies change the world
…Slide26
Investment and Investment Return Guarantees
We guarantee the following:
output parameters
operational stability of Lentikats Biocatalyst and its activity and productivitySlide27
Our partners in
the field of
industrial ap
p
lications
Fermenta
Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610,
Maharashtra
, India
www.fermentabiotech.com
Protean
, Pod Lesem 9, 373 16 Dobra Voda u Ceskych Budejovic, Czech Republic
www.protean.cz
Pharmix
s.r.o., Na
Sadkach
2798, 767 01
Kromeriz
,
Czech
Republic
www.
pharmix.czSlide28
Our partners in
the field of
research and development
Fermenta
Biotech
, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India
www.fermentabiotech.com
Institute of Microbiology
,
Academy of Science of the Czech Republic
Vídeňská 1083, 142 20 Prague 4, Krč, Czech Republic
www.biomed.cas.cz
STU Bratislava,
Fakulta chemické a potravinářské technologie, Ústav biotechnologie a potravinářství,
Radlinského
9, 81237 Bratislava,
Slovakia
www.stuba.sk
VŠCHT Praha,
Ústav kvasné chemie a bioinženýrství, Technická 5, 166 28 Praguea 6
www.vscht.cz
University of Alberta,
Edmonton Alberta, T6G 2J9 Canada
www.ualberta.ca
Masaryk University
,
National
Centre
for
Biomolecular
Research
, Kamenice 5, 625 00
Brno
-Bohunice
www.
ncbr.chemi.muni.czSlide29
Thank you for attention
Company
Headquarters
:
Office
Building ARGOLentiKat‘s a.s.Evropska 846/176a160 00 Prague 6Czech RepublicTel.: + 420 255 710 680 Fax: + 420 255 710 699E-mail: info@lentikats.euProduction plant
:
LentiKat‘s
a.s
.Pod
Vinicí
83471 27
Str
áž pod Ralskem
Czech
Republic
Tel.: + 420 487 851 393
Fax: + 420 487 851 399
E-mail: info@
lentikats.eu
Laboratory:
INBIT LentiKat‘s a.s.
Kamenice 34
625 00 Brno
Czech Republic
Tel.: + 420 511 205 288
Tel.: + 420 511 205 287