PPT-Animal tissue culture Lec.4

Animal tissue culture is the growth of tissues separate from the animal in vitro in the laboratory culture media Cell line Every cell present in the human body is not capable of growing in laboratory only a few types of cells can grow . Dr Gina Barnett. Café . Cardiologique. . 29. th. January 2014. Tissue Culture. In vitro cultivation of:. Organs (organ culture). Tissues (explant culture). Cells (cell culture). Rat aortic explant culture. Models in Tissue Engineering. March 29, 2016. Why the need of animal models in tissue engineering?. Preclinical . Investigation. Considerations with preclinical investigation requirements:. Medical. Legal. WHAT IS CELL CULTURE ?. Cell culture . is the process by which cells are grown under controlled conditions, generally outside of their natural environment.. ADVANTAGES :. Development of cell line over many generations.. introduction and history of acc . CELL CULTURE…... . In vitro culturing of animal cells, tissue fragment, organ or part of organ under aseptic condition using appropriate media….. ANIMAL TISSUE . Fig. 1-15. KOCH’S POSTULATES. Tools:. Diseased. animal. Healthy. animal. Red. blood. cell. Observe. blood/tissue. under the. microscope. Red. blood. cell. The Postulates:. 1. The suspected pathogenic. PH.... optimum ph is 7.4.. Vary for different cell strain.. Plant tissue-5.5. Epidermal tissue-5.5. Transformed tissue-7-7.4. Fibroblast-7.4-7.7. Phenol red used as a indicator.. Temperature. ….. Depend on body temperature of animal from which cell is obtain.. Tissue culture is the general name for the removal of cells, tissues or organs from an animal or plant and their subsequent placement into artificial environment conductive to growth. This environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semi-solid support medium that supplies the nutrients essential for survival and growth. The term tissue culture may be defined as the process of in-vitro culture of . explants. in nutrient medium under aseptic conditions. It includes the culture of cell, organ culture and suspension culture etc.. Plant . Tissue Culture is the process of growing isolated plant cells or organs in an artificial nutrient media outside the parent organism. In other words, it is an in vitro culture of plant cells or tissues on an artificial nutrient media under aseptic conditions, in glass containers.. Safina. . Aftab. Amal. . Awais. Bakhtawar. Ali. Hina. . Saif. Group Members. What Is Tissue Culture?. Tissue culture is a process that involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile, in vitro conditions to produce many new plants, each a clone of the original mother plant, over a very short period of time. tissue culture. ?. Plant . tissue culture. . is a . technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium. WHY?. The production of . (Animal Biotechnology). Anil Gattani/Ajeet Kumar. Make . proteins:. commercial. scale. Stem. and . cancer. cells. CELL . CULTURE. Primary. Human. Antibody. production:. Why. . do. . it. . ?. and . Subject – Pharmacognosy and . Phytochemistry. . By : . DR.Darshan. . Dubey. . Sources of Crude Drugs – Plant, Animal, Marine and Tissue Culture . Sources of Crude Drugs . CRUDE DRUGS. C. rude drugs are the drugs, which are obtained from natural sources like plant, insects, animals and minerals. They should be used as such as they occur in nature without any processing except, collection, drying and size reduction.. Plant-tissue . culture is . in-vitro . cultivation . of . plant . cell . or tissue under . . aseptic. . and. . controlled. . environment. conditions,. . in. . liquid. or. . on . . semisolid .