Vaccines and Cancer: The - PowerPoint Presentation

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Vaccines and Cancer: The Health Risks From Vaccine Cell Lines and Adventitious Contaminants

Dr. Sherri Tenpenny, DO, AOBNMM, ABIHM

Cleveland, Ohio USA

November 9,

2019

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DISCLAIMERThis

presentation is intended for educational purposes for course participants only.

The

information presented reflects the author’s opinions at the time

the presentation was created.

Some

information will change over time, as new research, data and information become available.

The

author assumes no responsibility for updating information that may modify any information presented

herein. All

rights

reserved. Copyright 2019.

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What We Are Going To Discuss Today Cell lines and serum used to manufacture vaccines

The potential risks of cell lines and their viral contaminants

Why multiple benign viral contaminants can lead to virulent recombinants

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Partial list of vaccine ingredients in commonly given vaccinesAluminum (4 forms)FormaldehydeGlutaraldehyde

2-phenoxyethanol

Polysorbate 80

Neomycin,

polymyxin

B

Lactose, casein

Sodium borateYeast proteins and Yeast DNABeta-propiolactone Triton X-100  Ethylene oxideThimerosal

Animal cellsBovine serum (several forms)Avian serum - chickentEgg protein – ovalbumin VERO cells – monkey Dog kidney cells (MDCK)Insect cells Human cellsWI-38MRC-5PER.C6

>NO TESTING FOR ANTIBODIES

>NO TESTING FOR SYNERGISTIC TOXICITY

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Problematic Ingredients #1: COW PROTEINS

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Bovine Ingredients in Vaccines Bovine casein (milk protein)Bovine extractBovine cassation acidsBovine serum albumen

Bovine calf serum

Bovine formula fed calf serum

Bovine calf serum protein

Fetal bovine serum (FBS)

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What Cow Parts are in Vaccines? Casein and lactose – Cow’s milkGlycerol - Tallow (fat)

Gelatin

–

Connective tissue, bones, tendons

Galactose

–

Circulating red blood cells Serum – Blood minus cellsAccording to the FDA: “Cow components are often used

simply because cows are very large animals… and thus much material is available.” REFERENCE: FDA - https://www.fda.gov/vaccines-blood-biologics/questions-about-vaccines/bovine-derived-materials-used-vaccine-manufacturing-questions-and-answers

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Cow SerumIndustry standards classify the serum according to the following guidelines:

Description of Age

Age Serum is obtained

Fetal bovine serum

Intra-uterine fetus

Newborn calf serum

Less than 21 days old

Formula-fed calf serum

Less than 22 weeks

old Calf bovine serum

21 days to 12 months old

Adult bovine serum

12 months or older

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Fetal Bovine SerumSerum is a complex mixture and even after 50 years of use, the majority of subunits have not been fully identified. Fetal bovine serum

(FBS) is the most widely

used,

the most difficult to obtain and the most

expensive of all

cell culture

promoter

substnances used in drug manufacturing 2014: FBS was found to contain

approximately 1,800 different proteins and more than 4,000 metabolites. The proportions of each vary between different batches and vary by the health of the animal from which it is harvested REFERENCE: Tanga, Fen, et al. “Fetal bovine serum influences the stability and bioactivity of resveratrol analogues: A polyphenol-protein interaction approach.” Food Chemistry, Volume 219, 15 March 2017, Pages 321-328

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WARNING: Disturbing ContentCows are occasionally sent to slaughter to thin the herd or because they are lame. If a cow is found to be pregnant during the removal of its internal organs, the uterus is quickly moved to a specialized extraction area where the fetal blood can be harvested within 30 minutes of the mother’s demise.

This

is done by inserting a sterile vacuum collection apparatus directly into the heart

of the fetal cow without

anesthetic. The fetus must be at least 3 months of age or the heart is too small for puncture

.

A 3-month fetus yields about 150ccs of serum while a near-term fetus (9 months) can yield up to 550ccs. The heart must be beating to ensure the blood remains uncoagulated and all blood can be extracted from the fetus; therefore, it is presumed that the fetus is alive at the time of the extraction.

After the blood is removed, the remains of the fetus are processed for animal feed.

REFERENCE: Carlo E. A. et al., “The use of fetal bovine serum: ethical or scientific problem?” ATLA 30, 219-227. 2013

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Bovine Sera Contaminants

Nearly 100% of commercially available

of bovine sera

is contaminated with species from the family of

Pestiviruses

Known contaminants in Fetal Bovine Serum:

Bovine diarrhea

virus (BVDV-1 and BVDV-2)

Classic swine fever virus (CSFV) Border disease virus (BDV)Bovine herpesvirus-4 and herpesvirus-8Hobi-like viruses 2015

: Italian study found that all 26 batches of FBS tested were contaminated with at least one species of bovine Pestivirus

REFERENCE:

Giammarioli

, M,

Ridpath

JF, et al. “Genetic detection and characterization of emerging

HoBi

-like viruses in archival

foetal

bovine serum batches.”

Biologicals

43, 220-224.

2015

.

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Bovine Sera ContaminantsAre

cow viruses

crossing

species

line

to cause

illness in humans?

 BVDV-antigens were found in the stool of 30 out of 128 children with infantile gastroenteritis.

REFERENCE: Yolken, Robert, et al. “Infantile gastroenteritis associated with excretion of Pestivirus antigens.” Lancet (8637):517-20 · April 1989Microcephaly has been reported in infants born to mothers who were seropositive for BVDV.REFERENCE: Erickson GA, et al. “Viral contamination of fetal bovine serum used for tissue culture: risks and concerns.” Dev Biol Stand. 75:173-5. 1991

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Vaccines with Bovine Products

Bovine Albumin

Calf Serum

Bovine Casein

Bovine Extract

Caseamino

Acids

Fetal

Bovine Serum (FBS)Vaqta PediarixPediarix

PediarixDaptacelAll

DTaP -

Tdap

Jap

Encep

Kinrix

Kinrix

Kinrix

Adacel

(teen)

Polio

Quadracel

Polio

Infanrix

Infanrix

Quadracel

MMR

Pentacel

Zostavax

Boostrix

Boostrix

Pentacel

ProQuad

–

MMR+

Varivax

RabAvert

DT

Td

Td

Rotateq

Typhoid

Menomune

Rabies

Menactra

Hepatitis

A

Prevnar

Varivax

-

Zostavax

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Problematic Ingredients #2: EGGS and CHICKEN

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EGGS as INCUBATORSEggs have been used to manufacture vaccines for more than 70 years. 11-day old chick embryos are injected with viral solutions.3-5 days later, the gooey viral suspension is removed and centrifuged several times.

Despite

all efforts, chicken blood,

chicken protein

and chicken proteins remain in

the final

product

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Avian (chicken) sourcesUsed in the production of

Influenza

,Rabies

and Yellow

fever

vaccines; some MMR vaccines

Known avian contaminants

include:

Avian leukemia virus…AVL Avian sarcoma virus Avian myeloblastosis virus Avian erthroblastosis virus Avian

myelocytomatosis virus REFERENCE: S Johnson, Eric, et al. “Cancer mortality in poultry slaughtering/processing plant workers belonging to a union pension fund.

” Environmental

Research.

Vol

588. Issue 94. August 01,

2010

Both viruses have been assoc. with

breast cancer

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Avian Viral contaminants

Both viruses have been assoc. with

breast cancer

“Considering that ALV

can

easily

capture the human oncogenes [called] “

erb-B” and “myc” and these two oncogenes are

strongly associated with common forms of human breast cancer, it seems that the issue of ALV vaccine contamination should deserve a high level of

attention.”

REFERENCE:

McRearden

, Benjamin. “What Is Coming Through That Needle? The Problem of Pathogenic Vaccine Contamination,” Townsend Letter, October (2003).

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18

Why

Are Viral Contaminants

A

C

oncern

?

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Recall: Bovine and Avian Contaminants Known Fetal

Bovine

Serum contaminants

Bovine diarrhea virus (BVDV-1 and BVDV-2)

Classic swine fever virus (CSFV)

Border disease virus (BDV)

Bovine herpesvirus-4 and herpesvirus-8

Hobi

-like viruses Known avian contaminants include: Avian leukemia virus… “parent virus” Avian sarcoma virus

Avian myeloblastosis virus Avian

erthroblastosis

virus

Avian

myelocytomatosis

virus

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20

100

particles

Benign Herpes Virus

A

100

particles

Benign Herpes Virus

B

Mouse #1

Mouse #2

Mouse ok

Mouse ok

ONE

particle

Benign Herpes Virus

A

+

ONE

particle

Benign Herpes Virus

B

Mouse #3

62% of MICE DIE

11 new recombinant

Viruses isolated

3 LETHAL in 4

th

gen.

Javier, RT,

et.al

. Two

avirulent

herpes simplex viruses generate lethal

combinants

in vivo.

Science.

1986 Nov 7; 234(4777):746-8.

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21

Conclusion:

Two

avirulent

viruses interact

in vivo to produce

virulent recombinants

that

can be

lethal to the next generation.

REFERENCE:

Javier

, R-T;

Sedarati

, F; Stevens, JG. Two

avirulent

herpes simplex viruses generate lethal recombinants in vivo.

Science.

1986 Nov 7; 234(4777): 746-8.

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Problematic Ingredients #3:Monkey Kidneys

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SV40 and CancerIn 1955

, Jonas Salk discovered how to mass produce polio vaccine by growing it in kidney tissues of African green monkeys.

By 1960:

R

esearchers had isolated simian viruses as a contaminant in vaccines. SV-40 is benign in monkeys, but was found to cause brain tumors in human tissue cultures.

1962:

The VERO cell line was derived by Japanese researchers from the kidney of adult African green monkey

VERO cell line is a continuous cell line which will grow indefinitely in cultureIt was estimated that between 1955 and 1963, 98 million Americans and millions of persons around the world received doses of live polio virus vaccines potentially tainted with SV-40.

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SV40 and Cancer1990s: Michael Carbone, Asst. Professor of Pathology at Loyola University in Chicago, isolated fragments of the SV-40 virus in the following cancers

:

Human bone cancers

33% of osteosarcomas

40% of other bone cancers

Lung cancer

60% of mesotheliomas tested had SV-4050% of mesotheliomas are no longer associated with asbestosREFERENCE: The Virus and The Vaccine: The True Story of a Cancer-Causing Monkey Virus, Contaminated Polio Vaccine, and the Millions of Americans Exposed by Debbie Bookchin

and Jim Schumacher. St. Marrtin’s Press. 2005.

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SV40 and Cancer1990s: An Italian research team, lead by Dr. Fernanda Martini, discovered SV-40 by reverse transcription PCR in:

other tumors, including a variety of brain tumors:

83% of

choriod

plexus

papillomas

73% of

ependymomas

50% of

glioblastomas

47

% of

astrocytomas

14

% of

meningiomas

None of the 13 normal brain tissue samples were positive for SV40 DNA

REFERENCE:

Martini

, Fernanda, et al. “SV4O Early Region and Large T Antigen in Human Brain Tumors, Peripheral Blood Cells, and Sperm Fluids from Healthy Individuals.”

iCancer

Research 56. 4820-4825. Oct 5, 1996.

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SV40 and Cancer Martini’

s research team also found SV-40 in

23% of peripheral blood samples

and

45% of sperm samples

taken from disease-free individuals

SV-40 has appeared

in 61% of all cancer

in patients too young to have received the contaminated vaccine Conclusion: SV40 virus may be transmitted sexually, through cord blood and through blood transfusions, from parent to childREFERENCE: Martini, Fernanda, et al. “SV4O Early Region and Large T Antigen in Human Brain Tumors, Peripheral Blood Cells, and Sperm Fluids from Healthy Individuals.” iCancer Research 56. 4820-4825. Oct 5, 1996

.

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Between 1997 and early 2002, more than 25 published studies found SV40 in human mesotheliomas; 16 others found the virus in brain and bone cancers, lymphomas, and other cancers and in kidneys and peripheral blood.

By

2003

, SV40 had been found in human

tumors

in 18 developed countries. T

he

rates of SV40-positive tumors seem highest in countries that used the greatest amount of contaminated Salk polio vaccine, including the UK, USA, and Italy.

REFERENCE: The Virus and The Vaccine: The True Story of a Cancer-Causing Monkey Virus, Contaminated Polio Vaccine, and the Millions of Americans Exposed by Debbie Bookchin and Jim Schumacher. St Maartin Press. 2005.

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For Example:2002: Non-

Hodgkins

lymphoma

tumors

42%

of 154 non-Hodgkin lymphomas. No SV40 was detected in 186 non-malignant

samples

or in the 54 control cancers.

REFERNCE: Malkin, David. “Simian virus 40 and non-Hodgkin lymphoma.” Lancet. Vol. 359. Issue 9309. Pg. 812-13. March 9, 2002. 43% of 68 non-Hodgkin lymphomas, 9% Hodgkin’s lymphomas. Viral sequences were absent in 40 control tissues. REFERENCE: Vilchez, Regis et al. “Association between simian virus 40 and non-Hodgkin lymphoma.” Lancet. Vol. 359. Issue 9309. Pg 817-823. March 9, 2002

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Then in 2004: “The SV40 virus is a known oncogenic DNA virus…

. Persuasive evidence now indicates that

SV40 is causing infections in humans today

and represents an emerging pathogen. A meta-analysis of data from 1,793 cancer patients indicates there is a significant excess risk of

SV40 association

with human primary

brain

cancers, primary

bone cancers, malignant mesothelioma, and non-Hodgkin's lymphoma.”REFERENCE: Vilchez

and Butel. “Emergent Human Pathogen Simian Virus 40 and Its Role in Cancer.” Clinical Microbiology Reviews. Jul; 17(3): 495–508

.

2004.

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Most Recently, 2019: The association between SV40 and human tumors is based on results obtained by many investigators.

Most

of these studies detected SV40 DNA sequences, using qualitative PCR techniques, in tumor specimens.

REFERENCE

:

Rotondo

JC, et al. “Association Between Simian Virus 40 and Human Tumors.” 

Front Oncology. 2019;9:670. July 25, 2019.

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Problematic Ingredients #4: Dog Cells

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MDCK Cells1958: Madin-Darby Canine Kidney

cell line

MDCK are kidney cells from

originally

derived from a

healthy female

cocker spaniel

Original line of MDCK cells was non-tumorigenic, but over time, some cells have been found to be highly

tumorigenicFDA: “Highly tumorigenic cell substrates pose significant regulatory challenges.”REFERENCE: FDA: Use of Madin-Darby Canine Kidney (MDCK) Cells for Manufacture of Inactivated Influenza Vaccines, Philip Krause, MD. (ppt presentation) 2017

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Tumorigenic Cell Lines1998: Initial discussions regarding the use of cancer-causing cells to create vaccines 1999-2000 FDA Conclusions:

Unrecognized

adventitious agents

may be a

major concern

in

neoplastic cell substratesRisks from residual DNA were ‘perceived’

to be lowThe amount of foreign DNA is limited to 10ng per injection Residual DNA may be infectious and/or oncogenic KEY: “

Previously used assays testing for tumorigenicity

may

not

have adequately defined

the tumorigenic

risk

associated with

residual tumorigenic cell.”

REFERENCE:

FDA

Briefing Document: “Vaccines and Related Biological Products Advisory Committee Meeting. Cell Lines Derived from Human Tumors for Vaccine Manufacture.” September 19, 2012

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Problematic Ingredients #4:Human Cells

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Cell Lines

MDCK

1958

Dog

kidney

FluCelVax

(approved 2016)

VERO

1962African Green Monkey

Kidney

Polio

RA

27/3

1964

Human

Kidney

Rubella fraction of MMR

WI-38

1965

Human lung

Varivax

,

Rubella

MRC-5

1970

Human lung

Varivax

,

Havrix

,

Vaqta

,

Twinrix

, Rabies, Shingles, Smallpox, [

Pentacel

]

PER.C.6

1985

Human

–

retina cells

Experimental

–

Ebola and HIV

Baculo

-virus

2013

Spodoptera

frugiperda

,

Armyworm

FluBlok

(approved 2014)

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MRC-5 Cells1966: The immortalized cell line, MRC-5,

was developed by British researchers at the Medical Research Council in England.

The

cells were derived from lung tissue of an aborted male at about 14-week gestation removed from a 27 year old woman for “psychiatric reasons.”

VACCINES:

Varivax, Havrix

, Vaqta, Twinrix, Rabies, Shingles, Smallpox, [Pentacel]

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WI-38 Cells 1962: Cells were extracted

from the lungs of an aborted female baby at approximately the end of the third month of pregnancy.

Many aborted fetus cell lines and various organs were tested

The 38

th

cell line was

found to be the most

adequate for immortalizationThe line has always been free of adventitious agents

Developed by Dr. Leonard Hayflick at the Wistar Institute in Philadelphia, PA – hence the name, WI-381965: WI-38 became commercialized REFERENCE: Medical research: Cell division. Nature. Vol 498, Issue 7455. June 26, 2013VACCINES; Rubella, Hepatitis A, Chickenpox, Rabieshttps://www.nature.com/news/medical-research-cell-division-1.13273

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WI-38 Cells 1961: “The Hayflick limit”

It was discovered

that

normal

fetal cells stop replicating after about 50 population doublings.

This became known as ‘The

Hayflick

limit.’These discoveries launched a new field: The study of cellular aging and the study of teleomeres. This 1961 paper became one of the most-ever cited publications in biology.

The full story of the development of the WI-38 cell line is part of medical history. It is quite interesting and even laced with intrigue. The full interview with Dr. Hayflick on YouTube: An Afternoon with Dr. Leonard Hayflick Discussing Aging Research, Telomeres, and Telomerase (Pt 1 and Pt 2)

REFERENCE: Medical research: Cell division. Nature.

Vol

498, Issue 7455. June 26, 2013

https

://www.nature.com/news/medical-research-cell-division-

1.13273

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PER.C.6 Cells 1985: PER.C.6 cell lines

were obtained

from human embryonic

retinoblasts

and immortalized

While found to be only mildly

tumorgenic, the cells were found to form tumors if transferred to a recipient No current human vaccines are licensed with these cells, which are still considered to be experimental

Slide40

Significant problems with the residual immortalized human cells found in vaccines

Slide41

Open Letter from Dr. Theresa Deisher, PhD on Human DNA in Vaccines, April 2019

A little background on Dr.

Deisher

She is

President of the non-profit Sound Choice Pharmaceutical Institute.

Dr.

Deisher was the first person to discover adult cardiac derived stem cells, and has been a champion of

adult stem cell research, both professionally and privately for over two decades. Her research also focuses on the health risks of residual human fetal DNA contaminants and retroviruses found in pharmaceutical products, including some vaccines. She studies how these contaminants may be implicated in autism, autoimmune disease and cancer.

As the result of this work, her name is on 23 patents as the inventor.

Slide42

Problem with Human Cell Lines“Vaccines manufactured

using human fetal cell

lines

are heavily contaminated with human fetal

DNA.

Levels in our children can reach up to

5

ng/ml after vaccination, depending on the age, weight and blood volume of the child. That level is known to activate Toll-like receptor 9 (TLR9), which can cause autoimmune attacks

.”“The rubella portion of the MMR vaccine contains human derived fetal DNA contaminants of about 175 ngs, more than 10x over the recommended WHO and FDA threshold of 10ng per vaccine doseREFERENCE: Theresa

Deisher, PhD. “Open Letter to Legislators Regarding Fetal Cell DNA in Vaccines.” April 8, 2019.

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Problem with Human Cell Lines

Administration

of fragments of human, non-self DNA to a child

can

generate an immune response that

can

cross-react with the

child’s own DNA.Injecting children with human fetal DNA contaminants risks of causing

two well-established pathologies:Autoimmune disease: Foreign fetal human DNA triggers an immune response, leading the immune system to attack his/her own body.Insertional mutagenesis: fetal human DNA incorporates into the child’s DNA causing mutations. Gene therapy using as little as 1.9ng/ml of DNA fragments results 100% insertion into genomes of injected mice. The

levels of human fetal DNA fragments in the MMR, Varivax (chickenpox) and Hepatitis A

vaccines

contain much more than vaccines 1.9

ng

/ml.

Theresa

Deisher

, PhD

–

continued

…

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Problem with Human Cell Lines

“The

presence of both the high level contaminating fetal DNA as well as the

the presence of Human

endogenous retrovirus K (HERVK) 

in

the MMR vaccine is an unstudied risk with huge implications and dangers for individual and public

health.”To date there is evidence of HERV-K retrovirus activation in ovarian cancer, melanoma, breast, prostate, lymphomas, leukemia and sarcomas

.Threresa Deisher PhD - continued… Dr.

Deisher exposed HERV-K in MMRCorvelva

Laboratories exposed HERV-K in

Gardasil

Slide45

Oct. 1967:

Science

Magazine

"

In point of fact, we are practicing biological engineering on a rather large scale by

using

live viruses in mass immunization campaigns…

Crude virus preparations are vulnerable to frightful mishaps of

contamination...

"

Joshua Lederberg

Dept. Human Genetics

Standford

School of Med.

Slide46

1976:

Address at

the annual American Cancer Society

Science

Writers

Seminar

“

Immunization Programs against flu, measles, mumps, polio and so forth may actually be

seeding humans with RNA to form latent proviruses in cells throughout the body. “These latent proviruses could be molecules in search of diseases, including rheumatoid arthritis, multiple sclerosis, systemic lupus erythematosus, Parkinson's disease, and perhaps cancer.”

Robert

W. Simpson, M.D.

,

Rutgers University

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