only Osmolar Solution So to reaching equilibrate stage the water will move from A to B from high water concentration to low water concentration This movement of water called Osmosis ID: 934354
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Slide1
Lab 4Osmotic pressure
The Osmolar concentration mainly used in biology to explain the osmotic effect, We suggest the existence of a container divided by water permeable membrane to two sides A and B . water presence in both sides and glucose (1 M) in side B
only
Slide2Osmolar Solution
So to reaching equilibrate stage the water will move from (A to B) { from high water concentration to low water concentration } . This movement of water called
Osmosis
, and the power that mated on the membrane called
Osmotic
pressure
Osmolar Solution
H2O
A
1M Glucose
1
Osm
B
Slide4Osmolar Solution
●
Osmol
(
Osm
)
:-A unite used to explain the capability of a solution to make Osmotic pressure.
◙ Every (1 m) of glucose sheds osmotic pressure on membrane about (1
Osm
) this applies to all
non ionic
molecules
.So
i
f we put (2M) of glucose in the side B this will cause ↑ in water movement from A side to B side causing ↑ in Osmotic pressure.
Slide5Osmolar Solution
◙About the
ionic molecules
such as
Nacl
, when we dissolve
Nacl
in water
Nacl
molecules will break down to 2 ions .
Nacl
------------> 1 Na + 1
cl
1mole
---------> 1mol + 1mole = 2mole
Each ion will cause osmotic pressure of it′s on , so the total osmotic pressure equal to the summation of the osmotic pressure of all the ions in the solution.
Slide6Osmolar Solution
H2O
A
(1 Na + 1
cl
)
2M
2
Osm
B
Slide7Osmolar Solution◙ Osmotic pressure of all mammals cell is 0.3
Osm
{300
mOsm
} milliosmolar , if we put normal cell in solution of
0.3
Osm
of glucose .
Or 0.15
Osm
of
Nacl
This cell will be intact , this solution called
isotonic
solution
.
Slide8Osmolar Solution
Isotonic Solution
:- A solution with osmotic pressure equal to osmotic pressure inside mammals’ cells. {the cell remain intact}
Hypertonic Solution
:- A solution with osmotic pressure higher than the osmotic pressure inside the cell. {the cell will be Shrinks }
Hypotonic
Solution
:- A solution with osmotic pressure lower than the osmotic pressure inside the cell .{the cell will be destroyed }
Slide9Osmolar Solution
Ex } prepare 1
Osm
solution of
Nacl
,not that mw of
Nacl
is 58.5 .
Nacl
------------> 1 Na + 1
cl
58.5
÷ 2 = 29.5 gm /1 L
Ex} prepare 1
Osm
of Cacl
2
solution , mw 110.
Cacl
2
--------- > 1 Ca + 2
cl
110
÷ 3 = 36.6 gm /1 L
Ex } prepare 1
Osm
of glucose ,mw 180.