Ranno Nahku PhD Microbial Bioprocess Group Leader rannotftakeu 372 53 436109 wwwtftakeu About us TFTAK is a CRO that is designed to solve most complex problems in microbial cultivation and food product development We have smartest people clear vision and excellent equipment ID: 935400
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Slide1
Introduction to TFTAK’s Microbial bioprocess group
Ranno
Nahku
, PhD
Microbial Bioprocess Group Leader
ranno@tftak.eu
, +372 53 436109
www.tftak.eu
Slide2About us
TFTAK is a CRO that is designed to solve most complex problems in microbial cultivation and food product development. We have smartest people, clear vision and excellent equipment.
Our mission is
to provide excellent support in applied research and product development.
Center of Food and Fermentation Technologies (TFTAK)
2
Slide3Established in 2004
100+
employees
Annual revenue
5 M€
> 25
PhD theses defended
50+ partners Positively evaluated R&D organization3
Slide4Focus areas
Functional foods
Fermented foods
Meat and dairy alternatives
Sensory and consumer scienceNutrition studies
Shelf-life and packaging
Food Science
High throughput cultivation techniquesMicrobial communities (NGP in collaboration with DuPont/IFF)In vitro
studies of human gut microbiota
Bioprocess Optimization
High throughput metagenomic sequencing of microbial communities
Chemical (GC-MS, GC-O, LC-MS, UPLC-QQQ) and physical (a
w
, rheology, TPA, SEM) analyses
Analytics
4
Slide5Notable partners
5
Slide6TFTAK’s microbial cultivation service6
Slide7Simple bioinformatics (example)7
“%” – completeness of the pathway
Slide88
Custom pathway maps for every species:
visualize omics data and metabolic flux patterns
Glycolysis
Butyrate
Kettle, H.
et al.
(2015) ‘Modelling the emergent dynamics and major metabolites of the human colonic microbiota’, Environmental Microbiology, 17(5), pp. 1615–1630. doi: 10.1111/1462-2920.12599
Slide99
-8
-1
1
8
Log
2
fold changeGlycolysisButyrateProtein expression analysis.
Early / mid-exponential phase
Slide10Lactococcus lactis
growth in milk
TAM IV-48
Microcalorimetry example: growth in milk
or Total heat, J
Alternatives:
microtiter plate reader
(Biotek) and iCinac10
Slide11Bioreactors and lyophilization
Seven
Applikon’s
1L and one 7L system
Down stream processing and analysis:Concentrating with centrifugation and cryoprotectant addition.Liquid N2 fixation.Lyophilization or spray drying.Viability and other analysis:Out plating
Flow cytometry16S metagenomics for contamination check (in house).
11
Slide12Human gut model consortium12
Slide13Simplified gut model consortium
Pooled
25
human
gut bacterial speciesAim:Estimate the effect of commercial fiber on gut microbiota before human consumption.Determine the effect of dozens of different fibers in a standardized manner.It is not a replacement for human trials but helps in guiding our choices.
13
Slide14Serial batch approach
Base medium – Complex medium based on yeast extract,
casitone
and mucin
14Isothermal microcalorimetry: the heat is proportional with biomass concentration.
Slide15Individual strain biomass estimation in consortium based on heat and 16S metagenomics
15
Slide16ApplicationsPrediction of the dietary supplement effect on human fecal consortium. The effect of dietary fibers and fiber mixture. Establish a fiber-species relationship and develop specialized products.
The effect of a natural fiber source (
e.g.
apple cider waste) and its technical treatment (enzymatic, thermal, etc.).
Test your prebiotic and probiotic species in the competitive environment. Find out how to promote your probiotic strain.Evaluate the effect of inhibitors/drugs on species of interest in competitive environment.Find out how to promote the consortium against pathogenic species (up to BSL 2).16
Slide17Ranno Nahku, PhDMicrobial Bioprocess Group Leaderranno@tftak.eu
17
TFTAK is
always
looking for talent. Please do not hesitate to contact us at any time!
Slide18Partners
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