Translated By Emaan Chaudry Adaptation to low oxygen conditions hypoxia Transcriptional Responses Regulation of the gene F45D34 How do we figure out what is causing the f45d34 gene to be expressed ID: 933559
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Slide1
The discovery of a hif-independent mediator of transcriptional responses to hypoxia in round worms
Translated By Emaan Chaudry
Slide2Adaptation to low oxygen conditions (hypoxia):
Transcriptional Responses
Slide3Regulation of the gene: F45D3.4
Slide4How do we figure out what is causing the f45d3.4 gene to be expressed?
Slide5How does rna interference help us?
Slide6Step 1 – isolate transcription factor
Slide7Step 2 – transcription factor placed in feeding vector
Slide8Step 3 – expressing the transcription factor in the bacteria
Slide9Step 4 - activation of t7 rna polymerase:
Slide10Step 5 - Synthesis of the double stranded rna:
Slide11Step 6 - Feeding the round worms the bacteria:
Slide12Step 7 – Inactivation of the transcription factor
Slide13Identification of the 3 transcription factors:
tbx-38
l
in-40
b
lmp-1
Slide14Questions?
Slide15ReferencesAll figures and data were taken from the website I created and references can be found there:
http://www.people.vcu.edu/~chaudrye/Padmanabha-2015/
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