PDF-Proc.Natl.Acad.Sci.USAVol.82,pp.6414-6418,October1985BiochemistryDNAse

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ProcNatlAcadSciUSA8219856415fragmentwasisolatedfroma08agarosegelbyusingtheapparatusforpreparativegelelectrophoresisDNASequencingDNAsequencingusingdideoxynucleotideswasperformedasdescribedby

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Proc.Natl.Acad.Sci.USAVol.82,pp.6414-6418,October1985BiochemistryDNAse: Transcript

ProcNatlAcadSciUSA8219856415fragmentwasisolatedfroma08agarosegelbyusingtheapparatusforpreparativegelelectrophoresisDNASequencingDNAsequencingusingdideoxynucleotideswasperformedasdescribedby. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA83(1986)3195U3R[U311PUvir5DNALTR-RUSU+ViralRNABamHIXhoILTRydLTPrototypePIli3400(3200)NheINheILEZ3EIILIZ3~MTZIJL*3200NT0,2200NT-SVNEO*3000NT*2200NTMT-NBLTRdLTRSV-N"1C7272U3NheIPvu Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)inasignificantlyfasterreaction.Theformationrateofnativemoleculesonthisfastrefoldingchannelcorrespondstotherateexpectedforfoldingtooccurintheabsenceofpopulatedintermediate Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest

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