by Jacob B Geri James V Oakley Tamara ReyesRobles Tao Wang Stefan J McCarver Cory H White Frances P RodriguezRivera Dann L Parker Erik C Hett Olugbeminiyi O Fadeyi ID: 1042217
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1. Microenvironment mapping via Dexter energy transfer on immune cellsby Jacob B. Geri, James V. Oakley, Tamara Reyes-Robles, Tao Wang, Stefan J. McCarver, Cory H. White, Frances P. Rodriguez-Rivera, Dann L. Parker, Erik C. Hett, Olugbeminiyi O. Fadeyi*, Rob C. Oslund*, and David W. C. MacMillan*ScienceVolume 367(6482):1091-1097March 6, 2020主讲人：白松霖 2020311624
2. Microenvironment mappingProteomics GenomicsNeuroscienceAntibody target Protein-proteininteractionsSynapse dynamicstargetmicroenvironmentProfiling biomoleculesnear genetic loci
3. Unable to control acitivation siteacitivation site controlled by photocat.
4. The Excitation energy of donor should match the accepter’s ox/red potential, but not its excitation energy level.Electron transfer require a collision between donor and accepter0.5~1.0 nmFRET(Foster resonance energy transfer) NERT(None radiative energy transfer)Foster versus DexterGround State of PhotocatalystexcitationS1T1ISCGround State of SubstrateInduced Dipole interactionInduced Dipole mechanism5~10nm requiredT1Excitation energy level should be matched Dexter energy transferElectron TransferOX state+eliminationelimination
5. Catalyst Development
6. Labeling Test
7. Diffusion TestAgarose BeadIgGIgGCat.VEGFR2EGFRCat.Diffusion radius
8. BBμMapping on Live CellsCD45Cat.B
9. CD29CD45CD47μMapping on Live CellsCD45, CD29, CD47Different MicroenviromentCD47SELLCR21PTK7CD71GLUT1ITM1HLA-CCD98CD49DCD29TRBC1CD45TRAT1PDIA6PTPRKCD45APF52CD5HLA-ACD2CD87SPNCD31Jurkat T cell
10. CD30PD-L1CD300AμMapping on Live CellsPD-L1, CD30, CD300AShared MicroenviromentJY-PD-L1 B cellLSAMPROBO1CD49DHLA-ECD97PD-L1CD300ACD30CD40CD48BAFF-RCD36CD23CD21SRB1HLA-CHLA-ACD45JAG-1PTPRSAPNH
11. μMapping on Live CellsImmunocoprecipitationGene CoexpressionPD-L1CD48HLA-ASRB1BAFF-RCD36CD23CD300ACD21CD40HLA-CCD30μMap
12. BBBBBBTrans of Cis labeling with μMapPD-L1IrCD45IrImmune Synapse between APC (JY-PD-L1 B cell) and T-cell (Jurkat T-cell).PD-1Trans labelingSynapseCis labeling
13. Trans of Cis labeling with μMapTrans labelingCis labeling
14. μMap vs. PeroxidaseμMapPeroxidase
15. Thanks