Core E Laboratory Biomarkers Quantitative Pharmacology Neuroimaging and Neurobehavioral Characterization Core January 29 th 2015 Core Director Steven D Douglas MD CoDirector Ruben C Gur PhD ID: 1042221
Download Presentation The PPT/PDF document "Penn Mental Health AIDS Research Center" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
1. Penn Mental Health AIDS Research CenterCore E: Laboratory Biomarkers, Quantitative Pharmacology, Neuroimaging, and Neurobehavioral Characterization CoreJanuary 29th, 2015Core Director: Steven D. Douglas, M.D.Co-Director: Ruben C. Gur, Ph.D.Co-Director: Athena Zuppa, M.D., M.S.C.E.
2. Core E: Specific AimsLaboratory BiomarkersAim 1 The Core Immunology-Virology Laboratory performs assays of body fluids including plasma and CSF markers related to AIDS and leukocyte markers related to HIV/AIDS, psychiatric disease, and co-morbidities.Aim 2The Core maintains a specimen repository, which includes plasma, serum, cells, and DNA.
3. Core E: Specific Aims (continued)Quantitative PharmacologyAim 3The Quantitative Pharmacology Laboratory utilizes and develops bioanalytical methodology to detect drug and biomarker exposure in various biologic media. Biological assays are performed on cells, plasma, serum, urine, and CSFThe Core provides metabolomic and metabonomic support to identify potential novel biomarkers of drug activity, subject characteristics which correlate with responsiveness to therapy and time-dependent markers of disease progressionThe Core performs In Silico and ADME screens in order to examine the interaction between psychotropic drugs and anti-viral drugsThe Core provides pharmacokinetics, pharmacodynamics, and pharmacometric (PK/PD and PM) support to facilitate drug selection, target dosing, and dosing recommendations in all relevant Pilot Projects and interacts with the other Cores.
4. Core E: Specific Aims (continued)Neuroimaging and Neurobehavioral CharacterizationAim 4The Center for Neuroimaging in Psychiatry and the Brain Behavior Laboratory performs state of the art assessment of regional brain function in normative and clinical populations.
5. Core E: ServicesLabs by SystemImmune/InflammImmuneInflammStressCoagNeurodamageCardioGeneralFractalkineMIP-1αIFNγhsCRPCortisolD-dimerNFHFractalkineCBCIFN-γsCD14CD4/CD8LPS NFLsICAMGlucoseIL-1βsCD163 Neopterin sVCAMβ-hCGIL-6sICAM sCD163 Viral loadIL-8sVCAM Genomic DNAIL-10TNFα MCP-1TNFα-R1 Monocyte and NK flow cytometry panels are available, as appropriate, for Pilot Projects.
6. Core E: ServicesLabs by ComorbiditiesCardiacRenal/LiverPsychiatric Creatine KinaseCreatinineCortisolNaNaIL-6KKTNFαASTClhsCRPHDLCa IFN-γLDLBUNCD4/CD8TriglyceridesTotal bilirubin CholesterolDirect bilirubin GlucoseTotal protein ALP AST ALT CO2 Albumin
7.
8. Core E: ServicesPeripheral Blood Leukocyte Cell PhenotypesT cell phenotypes:Naïve T cells:CD3+CD4+ or CD8+CCR7+CD45RO-CD62L+CD95-Central memory T cells:CD3+CD4+ or CD8+CCR7+CD45RO+Effector memory T cells:CD3+ CD4+ or CD8+CCR7-CD45RO+Effector T cells:CD3+CD4+ or CD8+CCR7-CD45RO- Stem cell-like T cells:CD3+CD4+ or CD8+CCR7+CD45RO-CD62L+CD95+
9. Core E: ServicesPeripheral Blood Leukocyte Cell PhenotypesMonocyte and NK Cell phenotypesClassical monocytes:CD14 highCD16 lowIntermediate monocytes:CD14 highCD16 positive Non-conventional monocytes:CD14 lowCD16 highTypical NK cells, ~90% (mainly cytotoxic):CD56dimCD16 high NK cells precursor-like ~10% (more specialized on cytokine production):CD56 brightCD16 low